• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of cochlear hair cell damage due to homeostatic disruption

Research Project

Project/Area Number 18K09377
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56050:Otorhinolaryngology-related
Research InstitutionKyoto University

Principal Investigator

Katsuno Tatsuya  京都大学, 医学研究科, 研究員 (90527665)

Co-Investigator(Kenkyū-buntansha) 北尻 真一郎  信州大学, 学術研究院医学系(医学部附属病院), 特任准教授 (00532970)
山本 典生  京都大学, 医学研究科, 准教授 (70378644)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords内耳 / 有毛細胞 / 難聴 / 細胞内微細構造 / 変性 / 形態異常 / 細胞間接着 / タイトジャンクション / 内耳有毛細胞 / 恒常性 / 細胞変性
Outline of Final Research Achievements

To elucidate the mechanism of degeneration of inner ear hair cells, one of the major causes of hearing loss, we focused on inner ear homeostasis. We analyzed genetically engineered mice for structural proteins involved in the cytoskeleton and cell adhesion, which are necessary for maintaining the compartments in the inner ear. One of these proteins, TRIOBP, is involved in the cytoskeletal system. Its deletion causes abnormalities in the shape of the "rootlet" of stereocilia in the hair cells, resulting in deafness. The analysis of these transgenic mice revealed that the rootlet, which had been thought to have a uniform structure, has different domains, providing further insight into the relationship between intracellular microstructure and auditory function.

Academic Significance and Societal Importance of the Research Achievements

本研究において、難聴の表現型を示す、内部環境の維持に必要な細胞骨格や細胞接着関連分子の変異マウスを解析し、細胞内微細構造と聴覚機能の関係について更なる知見を得ることができた。
生体では内耳は骨の中に囲まれている上に、内部環境が厳密にコントロールされているため、その内部環境を壊さず、何か起きているかを調べる事は難しいが、本研究の結果は、ヒト難聴のメカニズムの一端を明らかにしており、難聴治療につながる内耳研究の知識基盤の形成に貢献するものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing2019

    • Author(s)
      Katsuno Tatsuya、Belyantseva Inna A.、Cartagena-Rivera Alexander X.、Ohta Keisuke、Crump Shawn M.、Omori Koichi、Ito Juichi、Frolenkov Gregory I.、Friedman Thomas B.、Kitajiri Shin-ichiro
    • Journal Title

      JCI Insight

      Volume: 4 Issue: 12 Pages: 1-21

    • DOI

      10.1172/jci.insight.128561

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アクチン束化タンパク質TRIOBP-5は聴覚に関わる有毛細胞の根の形成と維持に必須である2020

    • Author(s)
      勝野 達也
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 内耳有毛細胞の感覚毛の根の形成および聴覚における アクチン束化タンパク質TRIOBPの役割2019

    • Author(s)
      Tatsuya Katsuno, Inna A. Belyantseva, Koichi Omori, Juichi Ito, Gregory I. Frolenkov, Thomas B. Friedman, Shin-ichiro Kitajiri
    • Organizer
      日本分子生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Different compositions of TRIOBP isoforms on the stereocilia rootlets: one continuously uniform actin cytoskeleton structure2018

    • Author(s)
      Tatsuya Katsuno
    • Organizer
      第70回日本細胞生物学会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi