| Project/Area Number |
18K09384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
北原 糺 奈良県立医科大学, 医学部, 教授 (30343255)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 耳鳴 / 漢方薬 / 動物モデル / c-fos / 動物実験モデル / サリチル酸 / 牛車腎気丸 / 動物実験 |
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| Outline of Final Research Achievements |
We replicated a previously reported salicylic acid -induced tinnitus animal model and determined the conditions under which the most tinnitus behavior was observed. In this animal model, tinnitus behavior was suppressed in the tinnitus rats model induced by salicylic acid which were given the Japanese traditional herbal medicine(Goshajinkigan), as compared to those which were given distilled water.
Next, the effects of Goshajinnkigan on the brain were examined using c-Fos, a measure of neural activity. In the primary auditory cortex, dorsal secondary auditory cortex, dorsal cochlear nerve nucleus, and central nucleus of the inferior colliculus, Goshajinkigan suppressed the salicylic acid-induced increase in the number of c-Fos-expressing cells. It was found that Goshajinkigan may suppress tinnitus by inhibiting neural activity in these regions.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により牛車腎気丸がサリチル酸誘発耳鳴に対して動物行動学的、免疫組織化学的な検討の両方で抑制効果を及ぼすことを示した。これまで漢方薬の耳鳴への効果を動物実験により検証されたことはなく、世界で初めての試みであった。本研究は牛車腎気丸の耳鳴への有用性の根拠となるものであり、耳鳴治療法の確立の一助となりえるものである。また、耳鳴への内服薬の効果を検証する動物実験のモデルケースとなりうるもので、今後、別の内服薬や別の耳鳴を検討する研究につなげることも可能となる。
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