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Establishment of an experimental tinnitus animal model for the new treatment of tinnitus

Research Project

Project/Area Number 18K09384
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56050:Otorhinolaryngology-related
Research InstitutionNara Medical University

Principal Investigator

YAMASHITA AKINORI  奈良県立医科大学, 医学部, 学内講師 (50588522)

Co-Investigator(Kenkyū-buntansha) 北原 糺  奈良県立医科大学, 医学部, 教授 (30343255)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords耳鳴 / 漢方薬 / 動物モデル / c-fos / 動物実験モデル / サリチル酸 / 牛車腎気丸 / 動物実験
Outline of Final Research Achievements

We replicated a previously reported salicylic acid -induced tinnitus animal model and determined the conditions under which the most tinnitus behavior was observed. In this animal model, tinnitus behavior was suppressed in the tinnitus rats model induced by salicylic acid which were given the Japanese traditional herbal medicine(Goshajinkigan), as compared to those which were given distilled water.
Next, the effects of Goshajinnkigan on the brain were examined using c-Fos, a measure of neural activity. In the primary auditory cortex, dorsal secondary auditory cortex, dorsal cochlear nerve nucleus, and central nucleus of the inferior colliculus, Goshajinkigan suppressed the salicylic acid-induced increase in the number of c-Fos-expressing cells. It was found that Goshajinkigan may suppress tinnitus by inhibiting neural activity in these regions.

Academic Significance and Societal Importance of the Research Achievements

本研究により牛車腎気丸がサリチル酸誘発耳鳴に対して動物行動学的、免疫組織化学的な検討の両方で抑制効果を及ぼすことを示した。これまで漢方薬の耳鳴への効果を動物実験により検証されたことはなく、世界で初めての試みであった。本研究は牛車腎気丸の耳鳴への有用性の根拠となるものであり、耳鳴治療法の確立の一助となりえるものである。また、耳鳴への内服薬の効果を検証する動物実験のモデルケースとなりうるもので、今後、別の内服薬や別の耳鳴を検討する研究につなげることも可能となる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2022 2021 2020 2019

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Behavioral and Immunohistochemical Evidence for Suppressive Effects of Goshajinkigan on Salicylate-Induced Tinnitus in Rats2022

    • Author(s)
      Kitano Koichi、Yamashita Akinori、Sugimura Taketoshi、Okayasu Tadao、Sakagami Masaharu、Osaki Daisuke、Kitahara Tadashi、Saito Yasuhiko
    • Journal Title

      Brain Sciences

      Volume: 12 Issue: 5 Pages: 587-587

    • DOI

      10.3390/brainsci12050587

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Retrospective evaluation of secondary effects of hearing aids for tinnitus therapy in patients with hearing loss2020

    • Author(s)
      Yokota Yoshihiro, Yamashita Akinori, Koyama Shinji, Kitano Koichi, Otsuka Shintaro, Kitahara Tadashi
    • Journal Title

      Auris Nasus Larynx.

      Volume: 47(5) Pages: 763-768

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ménière's disease with unremitting floating sensation is associated with canal paresis, gravity-sensitive dysfunction, mental illness, and bilaterality2019

    • Author(s)
      Kitahara Tadashi, Sakagami Masaharu, Ito Taeko, Shiozaki Tomoyuki, Kitano Koichi, Yamashita Akinori, Ota Ichiro, Wada Yoshiro, Yamanaka Toshiaki
    • Journal Title

      Auris Nasus Larynx

      Volume: 46(2) Pages: 186-192

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temporal window of integration estimated by omission in bone-conducted ultrasound2019

    • Author(s)
      Okayasu Tadao, Nishimura Tadashi, Uratani Yuka, Yamashita Akinori, Nakagawa Seiji, Yamanaka Toshiaki, Hosoi Hiroshi, Kitahara Tadashi
    • Journal Title

      Neurosci Lett.

      Volume: 696 Pages: 1-6

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] Trials to establish our rat behavioral model of tinnitus using salicylate2022

    • Author(s)
      Kitano Koich1,2, Yamashita Akinori1, Okayasu Tadao1, Nagatani Yoshiki3, Kitahara Tadashi1, Saito Yasuhiko2
    • Organizer
      18th Japan-Korea Joint Meeting of Otorhinolaryngology - Head and Neck Surger
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サリチル酸誘発耳鳴ラットの c―Fos による評価2022

    • Author(s)
      北野公一1,岡安 唯1,山下哲範1,長谷芳樹2,杉村岳俊3,齋藤康彦3,北原 糺1
    • Organizer
      第7回耳鳴・難聴研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 当科耳鳴外来における拍動性耳鳴の検討とその取り扱い2022

    • Author(s)
      山下哲範,北野公一,岡安 唯,西村忠己,北原 糺
    • Organizer
      第7回耳鳴・難聴研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] サリチル酸誘発耳鳴モデルラットの牛車腎気丸投与による行動評価と c-Fos による評価2022

    • Author(s)
      北野公一,山下哲範,岡安 唯,北原 糺
    • Organizer
      第32回日本耳科学会総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] サリチル酸投与ラットに対する牛車腎気丸投与によるc-Fos発現の検討2022

    • Author(s)
      北野公一1,山下哲範2,岡安 唯2,長谷芳樹3,杉村岳俊4,齋藤康彦4,北原 糺2
    • Organizer
      第37回日本耳鼻咽喉科漢方研究会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 拍動性耳鳴を繰り返した脳硬膜動静脈瘻の一例2021

    • Author(s)
      山下 哲範、北野 公一、岡安 唯、西村 忠己、北原 糺
    • Organizer
      第6回耳鳴・難聴研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] ラット耳鳴モデルに対する漢方薬投与実験2021

    • Author(s)
      北野 公一、岡安 唯、山下 哲範、長谷 芳樹、杉村  岳俊、齋藤  康彦、北原 糺
    • Organizer
      第6回耳鳴・難聴研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] 耳鳴患者における連続周波数自記オージオメトリー2020

    • Author(s)
      山下哲範,北野公一,齋藤 修,岡安 唯,西村忠己,山中敏彰,北原 糺
    • Organizer
      第65回日本聴覚医学会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] サリチル酸ラット耳鳴モデルの再現と漢方薬投与によるラットへの影響2020

    • Author(s)
      北野公一,山下哲範,岡安 唯,北原 糺
    • Organizer
      第30回日本耳科学会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] 補聴器を用いた耳鳴に対する音響療法について2019

    • Author(s)
      横田尚弘,小山真司,山下哲範,北原 糺
    • Organizer
      第120回日本耳鼻咽喉科学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] ナビゲーション支援による内リンパ嚢開放術2019

    • Author(s)
      山中敏彰,大山寛毅,松村八千代,伊藤妙子,山下哲範,太田一郎,上村裕和,藤田信哉,北原 糺
    • Organizer
      第81回耳鼻咽喉科臨床学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 当科耳鳴外来の現状について2019

    • Author(s)
      山下哲範,西村忠己, 北野公一,北原 糺
    • Organizer
      第5回耳鳴・難聴研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 完全な形でキヌタ骨が外耳道皮下に脱出した外傷性耳小骨離断の1例2019

    • Author(s)
      山下哲範,西村忠己,藤田裕人,山中敏彰,北原 糺
    • Organizer
      第29回日本耳科学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] ラット耳鳴モデルを使用した漢方研究2019

    • Author(s)
      北野公一,山下哲範,長谷芳樹,岡安 唯,齋藤康彦,北原 糺
    • Organizer
      第35回日本耳鼻咽喉科漢方研究会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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