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Role of VEGF-alphaB-crystallin in regulation of choroidal thickness in diabetes

Research Project

Project/Area Number 18K09394
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56060:Ophthalmology-related
Research InstitutionHokkaido University

Principal Investigator

Kase Satoru  北海道大学, 大学病院, 講師 (60374394)

Co-Investigator(Kenkyū-buntansha) 神田 敦宏  北海道大学, 医学研究院, 特任講師 (80342707)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsαBークリスタリン / 網膜色素上皮 / 糖尿病網膜症 / 網膜色素上皮細胞 / 終末糖化産物 / VEGF / 脈絡膜 / diabetic retinopathy / choroid / alpha-crystallin / AGEs
Outline of Final Research Achievements

We performed in vitro assays in human RPE cells treated with high glucose. αB-crystallin mRNA levels were significantly decreased during culture with 50mM glucose to 24 hours in comparison to mannitol control. While the αB-crystallin mRNA levels unaltered during culture with H2O2 with/without high-glucose condition and hypoxic conditions. On the other hand, αB-crystallin protein concentrations remained undetectable throughout the time-course, which did not increase in the supernatants of RPE cells in high glucose stimulation.

Academic Significance and Societal Importance of the Research Achievements

αBークリスタリンは様々な機能を有し、VEGFの分子シャペロンであるも筆者らは報告してきた。しかしながら、糖尿病網膜症病態における網膜色素上皮(RPE)のαBークリスタリンの機能は全くわかっていない。本研究により、当初我々はαBークリスタリンは高血糖負荷に対するRPEに対する細胞保護的な作用を有するのではないかと想像し研究を行った。しかし、予想に反し、高血糖下で容易に分解される可能性を示した。今後はαBークリスタリンの発現を増加させる研究を行い、糖尿病網膜症におけるRPEの細胞障害を可能な限り軽減するよう努めたいと考えている。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (27 results)

All 2021 2020 2019 2018 Other

All Journal Article (15 results) (of which Peer Reviewed: 11 results,  Open Access: 6 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Factors based on optical coherence tomography correlated with vision impairment in diabetic patients2021

    • Author(s)
      Endo Hiroaki、Kase Satoru、Tanaka Hikari、Takahashi Mitsuo、Katsuta Satoshi、Suzuki Yasuo、Fujii Minako、Ishida Susumu、Kase Manabu
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 3004-3004

    • DOI

      10.1038/s41598-021-82334-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Choroidal Thickness in Diabetic Patients Without Diabetic Retinopathy: A Meta-analysis2020

    • Author(s)
      Endo Hiroaki、Kase Satoru、Saito Michiyuki、Yokoi Masahiko、Takahashi Mitsuo、Ishida Susumu、Kase Manabu
    • Journal Title

      American Journal of Ophthalmology

      Volume: 218 Pages: 68-77

    • DOI

      10.1016/j.ajo.2020.05.036

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between diabetic macular edema and choroidal layer thickness.2020

    • Author(s)
      Endo H, Kase S, Takahashi M, Saito M, Yokoi M, Sugawara C, Katsuta S, Ishida S, Kase M
    • Journal Title

      PLoS One

      Volume: 7 Issue: 1 Pages: e0226630-e0226630

    • DOI

      10.1371/journal.pone.0226630

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diabetic Cataract in Spontaneously Diabetic Torii Fatty Rats2020

    • Author(s)
      Kikuchi Kasumi、Murata Miyuki、Noda Kousuke、Kase Satoru、Tagawa Yoshiaki、Kageyama Yasushi、Shinohara Masami、Sasase Tomohiko、Ishida Susumu
    • Journal Title

      Journal of Diabetes Research

      Volume: 2020 Pages: 1-9

    • DOI

      10.1155/2020/3058547

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alteration of choroidal vascular structure in diabetic retinopathy2019

    • Author(s)
      Kase S, Endo H, Takahashi M, Saito M, Yokoi M, Ito Y, Katsuta S, Sonoda S, Sakamoto T, Ishida S, Kase M
    • Journal Title

      British Journal of Ophthalmology

      Volume: 104 Issue: 3 Pages: 417-421

    • DOI

      10.1136/bjophthalmol-2019-314273

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between choroidal structure and duration of diabetes.2019

    • Author(s)
      Endo H, Kase S, Ito Y, Takahashi M, Yokoi M, Katsuta S, Sonoda S, Sakamoto T, Ishida S, Kase M
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol

      Volume: 257 Issue: 6 Pages: 1133-1140

    • DOI

      10.1007/s00417-019-04295-1

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suppression of choroidal neovascularization and fibrosis by a novel RNAi therapeutic agent against (pro)renin receptor.2019

    • Author(s)
      Liu Y, Kanda A, Wu D, Ishizuka ET, Kase S, Noda K, Ichihara A, Ishida S
    • Journal Title

      Mol Ther Nucleic Acids

      Volume: 17 Pages: 113-125

    • DOI

      10.1016/j.omtn.2019.05.012

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Galectin-1 promotes choroidal neovascularization and subretinal fibrosis mediated via epithelial-mesenchymal transition2019

    • Author(s)
      Wu Di、Kanda Atsuhiro、Liu Ye、Kase Satoru、Noda Kousuke、Ishida Susumu
    • Journal Title

      The FASEB Journal

      Volume: 33 Issue: 2 Pages: 2498-2513

    • DOI

      10.1096/fj.201801227r

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 糖尿病網膜症における脈絡膜厚と糖尿病治療の関与2019

    • Author(s)
      加瀬 諭
    • Journal Title

      あたらしい眼科

      Volume: 36 Pages: 647-652

    • Related Report
      2019 Research-status Report
  • [Journal Article] Clinical Features of Japanese Patients With Anti-α-enolase Antibody?Positive Autoimmune Retinopathy: Novel Subtype of Multiple Drusen2018

    • Author(s)
      Ando Ryo、Saito Wataru、Kanda Atsuhiro、Kase Satoru、Fujinami Kaoru、Sugahara Michitaka、Nakamura Yosuke、Eguchi Shuichiro、Mori Shohei、Noda Kousuke、Shinoda Kei、Ishida Susumu
    • Journal Title

      American Journal of Ophthalmology

      Volume: 196 Pages: 181-196

    • DOI

      10.1016/j.ajo.2018.08.044

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alteration of layer thickness in the choroid of diabetic patients2018

    • Author(s)
      Endo Hiroaki、Kase Satoru、Takahashi Mitsuo、Yokoi Masahiko、Isozaki Chisato、Katsuta Satoshi、Kase Manabu
    • Journal Title

      Clinical & Experimental Ophthalmology

      Volume: 46 Issue: 8 Pages: 926-933

    • DOI

      10.1111/ceo.13299

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of the Epipapillary Membrane in Maculopathy Associated with Cavitary Optic Disc Anomalies: Morphology, Surgical Outcomes, and Histopathology2018

    • Author(s)
      Tanaka Atsushi、Saito Wataru、Kase Satoru、Ishijima Kan、Noda Kousuke、Ishida Susumu
    • Journal Title

      Journal of Ophthalmology

      Volume: 2018 Pages: 1-12

    • DOI

      10.1155/2018/5680503

    • NAID

      120006491523

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 加齢性疾患への対応Q&A.高齢者の前眼部に生じる腫瘍について教えてください2018

    • Author(s)
      加瀬 諭
    • Journal Title

      あたらしい眼科

      Volume: 33 Pages: 321-326

    • Related Report
      2018 Research-status Report
  • [Journal Article] 今日の治療指針 私はこう治療しているー2018

    • Author(s)
      加瀬 諭
    • Journal Title

      眼内腫瘍

      Volume: 60 Pages: 1501-1502

    • Related Report
      2018 Research-status Report
  • [Journal Article] 特集 黄斑円孔の最新レビュー 黄斑円孔手術におけるinverted ILM flap techniqueの評価2018

    • Author(s)
      加瀬 諭
    • Journal Title

      臨床眼科

      Volume: 72 Pages: 38-43

    • Related Report
      2018 Research-status Report
  • [Presentation] Choroidal circulation in radiation retinopathy2019

    • Author(s)
      Kase S, Noda K, Hirooka K, Hashimoto Y, Ishida S
    • Organizer
      The 52nd Annual Retina Society Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Flow Cytometry of IgG4-Related Ophthalmic Disease: Ocular Immunity and Inflammatory Diseases.2019

    • Author(s)
      Kase S
    • Organizer
      34th Asia-Pacific Academy of Ophthalmology Congress (APAO)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Flow Cytometry in the Diagnosis of Lymphoma and Lymphoproliferative Disorders.2019

    • Author(s)
      Kase S
    • Organizer
      34th Asia-Pacific Academy of Ophthalmology Congress (APAO)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 糖尿病網膜症眼における脈絡膜構造解析2019

    • Author(s)
      加瀬 諭、遠藤弘毅、高橋光生、齋藤理幸、横井匡彦、伊藤有希、勝田 聡、園田祥三、坂本泰二、石田 晋、加瀬 学
    • Organizer
      第58回日本網膜硝子体学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Clinical features of primary vitreoretinal lymphoma in Hokkaido, Japan2018

    • Author(s)
      Kase S, Namba K, Iwata D, Mizuuchi K, Kitaichi N, Ishida S.
    • Organizer
      American Academy of Ophthalmology: Chicago, USA
    • Related Report
      2018 Research-status Report
  • [Presentation] Choroidal melanoma arising in pachychoroid2018

    • Author(s)
      Kase S, Noda K, Ishida S.
    • Organizer
      The Retina Society 51st Annual Scientific Meeting: San Francisco, USA
    • Related Report
      2018 Research-status Report
  • [Presentation] Immunohistochemical and immunocytochemical analyses in patients with vitreoretinal lymphoma2018

    • Author(s)
      Kase S, Namba K, Kannno-Okada H, Iwata D, Mizuuchi K, Fukuhara T, Fukuhara J, Kitaichi N, Matsuno Y, Ishida S.
    • Organizer
      36th World Ophthalmology Congress (WOC) 2018: Barcelona, Spain
    • Related Report
      2018 Research-status Report
  • [Presentation] 糖尿病黄斑浮腫と脈絡膜層別厚の関連2018

    • Author(s)
      遠藤弘毅、加瀬 諭、高橋光生、齋藤理幸、横井匡彦、菅原ちさと、勝田 聡、加瀬 学
    • Organizer
      第24回日本糖尿病眼学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 眼付属器悪性リンパ腫に対する放射線照射後の脈絡膜循環2018

    • Author(s)
      加瀬 諭、橋本勇希、木下留美子、石田 晋.
    • Organizer
      第35回日本眼循環学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 視神経乳頭黒色細胞腫のOCT angiography所見2018

    • Author(s)
      菊地 郁、加瀬 諭、石田 晋
    • Organizer
      第122回日本眼科学会総会
    • Related Report
      2018 Research-status Report
  • [Book] Neurofibroma and ganglioneuroma of the choroid. WHO Classification of Eye Tumours, 4th edition.2018

    • Author(s)
      Rodriguez F, Eagle RC, Moulin A, Croxatto JO, Kase S, Mudhar HS.
    • Publisher
      International Agency for Research on Cancer (IARC)
    • Related Report
      2018 Research-status Report
  • [Remarks] 北海道大学眼科ホームページ

    • URL

      https://eye.med.hokudai.ac.jp

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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