Development of glaucoma regeneration treatment method by intraretinal remodeling phenomenon
| Project/Area Number |
18K09406
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56060:Ophthalmology-related
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
國吉 一樹 近畿大学, 医学部, 准教授 (30234470)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 緑内障 / シナプトジェネシス / OFF層 / 接着分子 / 二光子顕微鏡 / シナプス接着分 / アストロサイト / 高眼圧マウスモデル / ライブイメージング / 錐体OFF反応 / 紫外線および緑LED / 薬理学的解析 / リモデリング / ON/OFF |
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| Outline of Final Research Achievements |
Our research aimed at the realization of regenerative medicine using intraretinal remodeling and a new drug discovery platform. There are OFF layer and ON layer in the inner layer of the retina, but it is known that the OFF layer is damaged in the early stage of glaucoma. We found the possibility of it happening in glaucoma model mouse. Therefore, we searched for a marker that separates the OFF layer, and found that the cell adhesion molecule Necl-1 in the retina separates the pathway of the OFF layer. We analyzed where Necl-1 expressed in retina and how it work. We completed the basis of intraretinal remodeling observation by reporter mouse and multiphoton microscopy so we can now analyze the function of Necl-1 in synaptogenesis.
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| Academic Significance and Societal Importance of the Research Achievements |
我々は、Necl-1レポータマウスの高眼圧モデル上で、二光子顕微鏡を用いて生体観察することで、網膜内層の新らたなシナプス形成（シナプトジェネシス）とそれを促進する分子機序を解明できる準備を完成した。これにより、神経再生回復観察システムにおけるシナプトジェネシス現象が観察でき、その現象を促進する有効な薬物の探索システムを確立でき、有効性評価も容易になる。このことにより、これまで不可能であった緑内障の再生医療の一端を拓くことができる。
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Barium-induced toxic anterior segment syndrome2021
Author(s)
Matsushita Kenji、Kawashima Rumi、Hashida Noriyasu、Hamano Yuki、Harada Kazuo、Higashisaka Kazuma、Baba Koichi、Sato Shigeru、Huang Weiran、Matsumoto Hiroshi、Hamanaka Teruhiko、Quantock Andrew J.、Nishida Kohji
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Journal Title
European Journal of Ophthalmology
Volume: 1
Issue: 3
Pages: 1-5
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Cross-Sectional Imaging Analysis of Epiretinal Membrane Involvement in Unilateral Open-Angle Glaucoma Severity.2018
Author(s)
Sakimoto S, Okazaki T, Usui S, Ishibashi T, Oura Y, Nishida K, Miki A, Kawasaki R, Matsushita K, Sakaguchi H, Nishida K.
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Journal Title
Invest Ophthalmol Vis Sci.
Volume: 59
Issue: 15
Pages: 5745-5751
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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