| Project/Area Number |
18K09409
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56060:Ophthalmology-related
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
淺原 俊一郎 神戸大学, 医学部附属病院, 助教 (00570342)
橘 吉寿 神戸大学, 医学研究科, 准教授 (50373197)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 糖尿病網膜症 / アストロサイト / neurovascular unit / in vivoイメージング / 糖尿病 / 網膜変性 / イメージング |
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| Outline of Final Research Achievements |
We evaluated the damage to neurovascular unit and performed in vivo two-photon imaging of retinal glial cells in diabetic (InsCreTg; Pdk1flox/flox) mice. Immunohistochemistry disclosed that microglial activation as well as decrease in laminin thickness and ganglion cell axons proceed other cellular and extracellular matrix events in diabetic mouse retinas. Improvement of optical system including contact lens enabled high-resolution in vivo retinal imaging. Using this imaging system, we observed microglia migrating around retinal vessels and astrocytes with its processes extending towards them and detected leukocytes slowly moving in the retinal vessel lumen.
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| Academic Significance and Societal Importance of the Research Achievements |
糖尿病網膜神経変性は検眼鏡的に明らかな糖尿病網膜症が出現する以前から網膜内層に生じている神経変性であり、細胞レベルではこの段階でneurovascular unitの障害が生じていると考えられている。本研究によって糖尿病早期における細胞レベルでの網膜機能評価を可能にするする技術が確立されれば、早期からのneurovascular unit障害の評価が可能となり、結果的に糖尿病網膜症による失明予防に大いに貢献できると考える。
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