| Project/Area Number |
18K09410
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 56060:Ophthalmology-related
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
Morizane Yuki 岡山大学, 医歯薬学総合研究科, 教授 (50432646)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
大内 淑代 岡山大学, 医歯薬学総合研究科, 教授 (00253229)
米澤 朋子 岡山大学, 医歯薬学総合研究科, 助教 (30304299)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 網膜色素上皮細胞 / 加齢黄斑変性 |
|---|
| Outline of Final Research Achievements |
Age-related macular degeneration (AMD) is an intractable ocular disease that leads to blindness, and the number of patients with AMD is expected to increase rapidly. In this study, we targeted sirtuins (SIRTs) and KCNJ13, one of the potassium channels in RPE, among the genes thought to be related to the function of RPE, and aimed to clarify the effects of these genes on the function of RPE using genome editing technology. As for KCNJ13 gene, we found that the KCNJ13 gene in iPS-RPE is regulated by genome editing, but not by genome editing. Since the RPE is essentially a monolayer epithelial cell that forms tight junctions and functions as a blood-eye barrier, the deletion of the KCNJ13 gene may affect other RPE and retinal functions. Therefore, the deletion of the KCNJ13 gene may affect other RPE and retinal functions, and may be involved in the pathogenesis of AMD.
|
| Academic Significance and Societal Importance of the Research Achievements |
網膜色素上皮細胞におけるKCNJ13遺伝子の役割をゲノム編集によって得たiPS-RPEを用いて解明した。 網膜色素上皮細胞はタイトジャンクションを形成し血液眼関門の機能を有することから、KCNJ13遺伝子の異常が様々な網膜疾患の病態に関与している可能性が示唆された。今後、本研究の成果を基盤としてKCNJ13遺伝子の機能がさらに解明されることが期待される。
|
