| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
To establish a cultivation method with the limbal epithelial stem cells niche, we performed spheroidal cultivation using methylcellulose, and to investigate whether limbal organoids were engrafted to the limbus, we transplanted human limbal organoids in a rabbit limbal deficiency model.Limbal organoids with a limbal phenotype can be maintained for up to 1 month using methylcellulose. Limbal organoids were successfully engrafted in a rabbit limbal deficiency model. Immunohistochemistry against human nuclei confirmed the presence of organoid-derived cells migrating on to host corneas. Our data suggested that limbal organoids are engrafted to the limbus of a rabbit limbal deficiency model and supply corneal epithelial cells, and that organoids may be an efficient cell source for clinical use.
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