Elucidation of mechanism of pain-relief by transplanting endothelial progenitor cells to treat critical limb ischemia

• Project/Area Number: 18K09493
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Juntendo University
• Principal Investigator: Hagiwara Hiroko 順天堂大学, 大学院医学研究科, 非常勤講師 (30589429)
• Co-Investigator(Kenkyū-buntansha): 田中 里佳 順天堂大学, 医学部, 教授 (70509827) ; 櫻田 司 第一薬科大学, 薬学部, 名誉教授 (80124907)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 再生医療 / 慢性虚血性疼痛 / 疼痛緩和 / 細胞移植 / 重症下肢虚血 / 血管内皮前駆細胞 / 疼痛軽減 / 細胞移植治療

Outline of Final Research Achievements

Our study elucidated a part of mechanism of pain-relief by transplanting endothelial progenitor cells to critical limb ischemia patients.

Academic Significance and Societal Importance of the Research Achievements

世界的な糖尿病罹患率の増加に伴い、重症下肢虚血患者数も増加しており、動物モデルの確立及び、患者QOLを著しく低下させる間性疼痛の軽減メカニズムの解明は、学術的にも社会的にも意義があるものである。

Research Products

• [Journal Article] Quality and Quantity?Cultured Murine Endothelial Progenitor Cells Increase Vascularization and Decrease Fibrosis in the Fat Graft (2019)
  • Author(s): Geeroms Maxim、Hamdi Moustapha、Hirano Rie、Hagiwara Hiroko、Fujimura Satoshi、Mizuno Hiroshi、Tanaka Rica
  • Journal Title: Plastic and Reconstructive Surgery Volume: 143 Issue: 4 Pages: 744e-755e
  • DOI: 10.1097/prs.0000000000005439
  • Peer Reviewed / Int'l Joint Research
• [Presentation] A potential new cell therapy using peripheral blood mononuclear cells via quality and quantity controlled culture for chronic ischemic pain (2019)
  • Author(s): Hagiwara H, Tanaka R, Sakurada T, Mizuno H
  • Organizer: Novel Pain Therapeutics: From Basic Research to Clinical Translation and Rehabilitation
  • Int'l Joint Research
• [Presentation] 生体外増幅末梢血単核球が神経再生過程に及ぼす効果の検証 (2019)
  • Author(s): 萩原裕子、田中里佳、水野博司
  • Organizer: 第18回日本再生医療学会
• [Presentation] 生体外増幅末梢血単核球が神経傷害時における神経伸長に及ぼす効果の検証 (2019)
  • Author(s): 萩原裕子、田中里佳、水野博司
  • Organizer: 第34回日本形成外科学会基礎学術集会
• [Presentation] 生体外増幅末梢血単核球（MNC-QQ細胞）の線維芽細胞遊走促進効果及 びそのメカニズム (2019)
  • Author(s): 西貝燕、萩原裕子、藤村 聡、水野博司、田中里佳
  • Organizer: 第49回日本創傷治癒学会
• [Presentation] 生体外増幅末梢血単核球が神経再生過程に及ぼす効果の検証 (2019)
  • Author(s): 萩原裕子
  • Organizer: 日本再生医療学会