| Project/Area Number |
18K09497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56070:Plastic and reconstructive surgery-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松本 太郎 日本大学, 医学部, 教授 (50366580)
長岡 悠紀 日本大学, 医学部, 研究員 (30789186)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 脱分化 / 脂肪細胞由来線維芽細胞 / 創傷治癒 / 成熟脂肪細胞 / adiponectin / ASC / 脱分化脂肪細胞 / DFAT / tdTomato / 間葉系幹細胞 / ペリサイト / 皮膚全層欠損 |
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| Outline of Final Research Achievements |
We observed the appearance of tdT-positive adipocyte-derived fibroblast-like cells (ADFs) at the site of skin injury in Adipoq-ERT2Cre/tdT mice, which are capable of tracking the fate of mature adipocytes. Some of the ADFs were positive for the cell proliferation marker Ki-67. Further, several ADFs expressed markers of reticular fibroblast, pro-adipogenic fibroblast, or pericyte.
Next, after collecting subcutaneous adipose tissue from tamoxifen-treated Adipoq-ERT2Cre/tdT mice, we tried to prepare ADFs in vitro by inducing dedifferentiation of mature adipocytes isolated by enzymatic treatment in a ceiling culture. The tdT-positive ADFs obtained by the ceiling culture method showed the similar adhesive and proliferative ability as adipose-derived stem cells (ASCs). Cell surface antigen analysis showed that Sca-1, CD29, and CD106 were highly positive as well as ASCs, while the positive rates of CD44 and CD105 were lower than those of ASCs.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において成熟脂肪細胞の運命追跡が可能な遺伝子改変マウスを用いて、皮膚傷害時に出現するADFの形質および機能解析を行なった。本研究成果は、難治性皮膚疾患などに対するin situ cell therapyの開発に結びつく見識を深めることに繋がる。また、生体内で自立的に組織を再生し、機能回復へ結びつく有効性・安全性の高い新たな細胞治療技術の確立を目指すものとして学術的・社会的意義のある研究であると考える。
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