Study on the physiological and pathological function of 8-nitro-cGMP in bone remodeling.
Project/Area Number |
18K09513
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 57010:Oral biological science-related
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Research Institution | Showa University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 骨リモデリング / 破骨細胞 / 骨芽細胞 / 一酸化窒素 / 活性酸素種 / 8-nitro-cGMP / 活性硫黄分子種 / 酸化ストレス / 活性イオウ分子種 / 骨細胞 / 骨形成 / 骨吸収 / 8-ニトロ-cGMP |
Outline of Final Research Achievements |
Bones' shape and mechanical strength are maintained through bone remodeling composed of bone resorption by osteoclasts and bone formation by osteoblasts. The roles of nitric oxide (NO) and reactive oxygen species (ROS) in bone remodeling have not been fully clarified. In this study, we examined the production and function of 8-nitro-cGMP, a novel signaling molecule of NO and ROS, in osteoclasts, osteoblasts, and osteocytes. All the three types of cells examined produced 8-nitro-cGMP. 8-nitro-cGMP promoted osteoclast differentiation and suppressed mineralization by osteoblasts. These results indicate that 8-nitro-cGMP is a regulator that supports bone resorption and suppresses bone formation.
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Academic Significance and Societal Importance of the Research Achievements |
8-ニトロ-cGMPが骨吸収を促進し、骨形成を抑制することが示唆された。生体内での8-nitro-cGMPの生成経路、シグナル伝達様式および不活性化経路は、すでに明らかになっている。骨粗鬆症では、骨吸収が骨形成に対して優位になることで骨量が減少する。8-ニトロ-cGMPの生成を抑制あるいは不活性化を促進することで、骨吸収を抑制し、骨形成を促進できれば、骨粗鬆症の病態を改善できる可能性がある。
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Zoledronate promotes inflammatory cytokine expression in human CD14-positive monocytes among peripheral mononuclear cells in the presence of γδ T cells.2021
Author(s)
Takimoto R, Suzawa T, Yamada A, Sasa K, Miyamoto Y, Yoshimura K, Sasama Y, Tanaka M, Kinoshita M, Ikezaki K, Ichikawa M, Yamamoto M, Shirota T, Kamijo R
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Journal Title
Immunology
Volume: 162(3)
Issue: 3
Pages: 306-313
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Functional analysis of PTH1R variants found in primary failure of eruption.2020
Author(s)
Izumida E, Suzawa T, Miyamoto Y, Yamada A, Takimoto R, Otsu M, Saito T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Yoshimura K, Sasa K, Takimoto R, Uyama R, Shirota T, Maki K, Kamijo R.
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Journal Title
Journal of Dental Research
Volume: 99
Issue: 4
Pages: 429-436
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Possible involvement of elastase in enhanced osteoclast differentiation by neutrophils through degradation of osteoprotegerin.2020
Author(s)
Sugisaki R, Miyamoto Y, Yoshimura K, Sasa K, Kaneko K, Tanaka M, Itose M, Inoue S, Baba K, Shirota T, Chikazu D, Kamijo R
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Journal Title
Bone
Volume: 132
Pages: 115216-115216
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Production of 8-nitro-cGMP in osteocytic cells and its upregulation by parathyroid hormone and prostaglandin E2.2019
Author(s)
Nagayama K, Miyamoto Y, Kaneko K, Yoshimura K, Sasa K, Akaike T, Fujii S, Izumida E, Uyama R, Chikazu D, Maki K, Kamijo R.
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Journal Title
In Vitro Cell Dev Biol Anim
Volume: 55
Issue: 1
Pages: 45-51
DOI
Related Report
Peer Reviewed
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