• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of epigenetic change in the epithelial cells induced by periodontopathic bacteria

Research Project

Project/Area Number 18K09559
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57020:Oral pathobiological science-related
Research InstitutionTokyo Dental College

Principal Investigator

Ishihara Kazuyuki  東京歯科大学, 歯学部, 教授 (00212910)

Co-Investigator(Kenkyū-buntansha) 菊池 有一郎  東京歯科大学, 歯学部, 講師 (30410418)
柴山 和子  東京歯科大学, 歯学部, 講師 (60408317)
国分 栄仁  東京歯科大学, 歯学部, 講師 (70453785)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsTreponema denticola / gene expression / epigenetic regulation / periodontitis / periodontal microbiome / エピジェネティクス / 歯周炎 / Porphyromonas gingivalis / 歯周病原菌 / エピジェネティック制御 / 歯肉上皮細胞 / 感染 / 慢性歯周炎 / 歯周病原性菌 / dysbiosis / タンパク分解酵素 / 歯周疾患 / マトリクスメタロプロテアーゼ
Outline of Final Research Achievements

By the metagenomic analysis of functional genes of the subgingival plaque, genes involved in carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism and replication and repair were changed. Among them, the genes involved in phenylalanine bio-synthesis was consistent with previous reports. In the epithelial cells infected by T. denticola, proteins involved in the migration were hydrolyzed. By ATAC-seq analysis, the DNA with regions of open chromatin was very small in cells infected by T. denticola compared with those by P. gingivalis and uninfected cells. These results suggested the regulation of gene expression in the epithelial cells by T. denticola and further analysis using mixed infection model is underway.

Academic Significance and Societal Importance of the Research Achievements

歯周炎は、歯肉溝内の菌種に、病原性の強いものが増えることによって引きおこされると考えられている。本研究では、歯周炎病巣から高頻度で分離されるT. denticolaは、同じ歯周炎に関わるPorphyromonas gingivalisと感染時の遺伝子発現を比べた。その結果、T. denticolaの感染した細胞では遺伝子発現の認められる部分が少なく、細胞の機能を抑制する事によって病原性を発揮している可能性が認められた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (34 results)

All 2021 2020 2019 2018

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 14 results,  Open Access: 8 results) Presentation (17 results) (of which Int'l Joint Research: 4 results) Book (3 results)

  • [Journal Article] Inhibitory Effect of Resveratrol on Candida albicans Biofilm Formation.2021

    • Author(s)
      Okamoto-Shibayama K, Yoshida A, Ishihara K.
    • Journal Title

      Bull Tokyo Dent Coll.

      Volume: 62(1) Issue: 1 Pages: 1-6

    • DOI

      10.2209/tdcpublication.2020-0023

    • NAID

      130007997849

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of hyalin-like protein in gliding and biofilm formation by Capnocytophaga ochracea2021

    • Author(s)
      Warita, T. Okamoto-Shibayama, K. Kokubu, E. KIta, D. Kikuchi, Y. Ishihara, K.
    • Journal Title

      The Bulletin of Tokyo Dental College

      Volume: 63

    • NAID

      130008048872

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of the Treponema denticola Virulence Factor Dentilisin2020

    • Author(s)
      Kikuchi Yuichiro、Ishihara Kazuyuki
    • Journal Title

      Methods Mol Biol

      Volume: 2210 Pages: 173-184

    • DOI

      10.1007/978-1-0716-0939-2_17

    • ISBN
      9781071609385, 9781071609392
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola2020

    • Author(s)
      Arai Yuki、Kikuchi Yuichiro、Okamoto-Shibayama Kazuko、Kokubu Eitoyo、Shintani Seikou、Ishihara Kazuyuki
    • Journal Title

      Journal of Oral Microbiology

      Volume: 12 Issue: 1 Pages: 1829404-1829404

    • DOI

      10.1080/20002297.2020.1829404

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigation of the antimicrobial activity of Bilberry (Vaccinium myrtillus L.) extract against periodontopathic bacteria2020

    • Author(s)
      Satoh Yutaroh、Ishihara Kazuyuki
    • Journal Title

      Journal of Oral Biosciences

      Volume: 62 Issue: 2 Pages: 169-174

    • DOI

      10.1016/j.job.2020.01.009

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synergistic biofilm formation by Parvimonas micra and Fusobacterium nucleatum2019

    • Author(s)
      Horiuchi Akira、Kokubu Eitoyo、Warita Takenobu、Ishihara Kazuyuki
    • Journal Title

      Anaerobe

      Volume: 62 Pages: 102100-102100

    • DOI

      10.1016/j.anaerobe.2019.102100

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inhibitory Effects of Lingonberry Extract on Oral Streptococcal Biofilm Formation and Bioactivity2019

    • Author(s)
      Kokubu Eitoyo、Kinoshita Emiko、Ishihara Kazuyuki
    • Journal Title

      The Bulletin of Tokyo Dental College

      Volume: 60 Issue: 1 Pages: 1-9

    • DOI

      10.2209/tdcpublication.2018-0007

    • NAID

      130007605372

    • ISSN
      0040-8891
    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dysbiosis of oral microbiota in palmoplantar pustulosis patients.2019

    • Author(s)
      Michiyoshi Kouno, Yurie Akiyama, Masaki Minabe, Naohiko Iguchi, Takeshi Nomura, Kazuyuki Ishihara, Shinichi Takahashi
    • Journal Title

      Journal of Dermatological Science

      Volume: 93(1) Issue: 1 Pages: 67-69

    • DOI

      10.1016/j.jdermsci.2018.12.003

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a specific domain of Porphyromonas gingivalis Hgp44 responsible for adhesion to Treponema denticola2018

    • Author(s)
      Yoshikawa Kouki、Kikuchi Yuichiro、Kokubu Eitoyo、Imamura Kentaro、Saito Atsushi、Ishihara Kazuyuki
    • Journal Title

      Pathogens and Disease

      Volume: 76 Issue: 5 Pages: 1-8

    • DOI

      10.1093/femspd/fty047

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of Porphyromonas gingivalis infection in the placenta and umbilical cord in pregnant mice with low birth weight2018

    • Author(s)
      Udagawa Sayuri、Katagiri Sayaka、Maekawa Shogo、Takeuchi Yasuo、Komazaki Rina、Ohtsu Anri、Sasaki Naoki、Shiba Takahiko、Watanabe Kazuki、Ishihara Kazuyuki、Sato Noriko、Miyasaka Naoyuki、Izumi Yuichi
    • Journal Title

      Acta Odontologica Scandinavica

      Volume: 15 Issue: 6 Pages: 1-9

    • DOI

      10.1080/00016357.2018.1426876

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Treponema denticola transcriptional profiles in serum-restricted conditions2018

    • Author(s)
      Tanno-Nakanishi Mariko、Kikuchi Yuichiro、Kokubu Eitoyo、Yamada Satoru、Ishihara Kazuyuki
    • Journal Title

      FEMS Microbiology Letters

      Volume: 365 Issue: 16

    • DOI

      10.1093/femsle/fny171

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] <i>Treponema denticola</i> Induces Epithelial Barrier Dysfunction in Polarized Epithelial Cells2018

    • Author(s)
      Kikuchi Yuichiro、Kimizuka Ryuta、Kato Tetsuo、Okuda Katsuji、Kokubu Eitoyo、Ishihara Kazuyuki
    • Journal Title

      The Bulletin of Tokyo Dental College

      Volume: 59 Issue: 4 Pages: 265-275

    • DOI

      10.2209/tdcpublication.2017-0052

    • NAID

      130007538122

    • ISSN
      0040-8891
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Corrosion behavior of titanium in response to sulfides produced by Porphyromonas gingivalis2018

    • Author(s)
      Harada Rino、Kokubu Eitoyo、Kinoshita Hideaki、Yoshinari Masao、Ishihara Kazuyuki、Kawada Eiji、Takemoto Shinji
    • Journal Title

      Dent Mater

      Volume: 34 Issue: 2 Pages: 183-191

    • DOI

      10.1016/j.dental.2017.10.004

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of a novel potential peptide import system in Treponema denticola2018

    • Author(s)
      Asai Tomohiro、Okamoto-Shibayama Kazuko、Kikuchi Yuichiro、Ishihara Kazuyuki
    • Journal Title

      Microbial Pathogenesis

      Volume: 123 Pages: 467-472

    • DOI

      10.1016/j.micpath.2018.07.045

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Comparison of microbiome organized on denture base materials and hydroxyapatite2020

    • Author(s)
      Nezu, Y. Ryu, M. Ishihara, K. Ueda, T.
    • Organizer
      The 68th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of hyalin-like protein in biofilm formation by Capnocytophaga ochracea2020

    • Author(s)
      Warita, T. Okamoto-Shibayama, K. Kokubu, E. KIta, D. Kikuchi, Y. Ishihara, K.
    • Organizer
      The 68th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CTLA-4 reduces bone resorption through the inhibition of osteoclast differentiation2020

    • Author(s)
      Nakane, S. Imamura, K. ishihara, K. Saito, A.
    • Organizer
      The 68th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 周炎モデルマウスの歯槽骨吸収におけるCTLA-4の役割の解明2020

    • Author(s)
      中根咲, 今村健太郎, 長野恭輔, 渡辺一夫, 石原和幸, 齋藤淳
    • Organizer
      第63回春季日本歯周病学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 血清制限下におけるTreponema denticolaの応答機構の解明2020

    • Author(s)
      深澤俊也 松上大亮 山下慶子 喜田大智 今村健太郎 勢島典 齋藤淳 石原和幸
    • Organizer
      日本歯科保存学会2020年度秋季学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Treponema denticolaにおける表層病原性成分の発現調節機構の解明2019

    • Author(s)
      北村友里恵, 菊池有一郎, 国分栄仁, 齋藤淳, 石原和幸
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Treponema denticolaの環境ストレス応答機構の解明2019

    • Author(s)
      深澤俊也, 北村友里恵, 山下慶子, 菊池有一郎, 国分栄仁, 奥山慶子, 藤本芳弘, 齋藤淳, 石原和幸
    • Organizer
      第62回秋季日本歯周病学会学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] An investigation of the role of a gene encoding a transcriptional regulator in Treponema denticola2019

    • Author(s)
      山下慶子, 国分栄仁, 菊池有一郎, 齋藤淳, 石原和幸
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] Porphyromonas gingivalis Hgp44のTreponema denticolaとの共凝集における付着ドメインの解明2019

    • Author(s)
      吉川幸輝, 菊池有一郎, 国分栄仁, 北村友里恵, 齋藤淳, 石原和幸
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Investigation of a novel DNA binding protein of Trepoenma denticola2019

    • Author(s)
      Kitamura Y, Yamashita K, Kikuchi Y, Fukasawa T, Saito A, Ishihara, K
    • Organizer
      97th General Session of the IADR
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] An investigation of the role of a gene encoding a transcriptional regulator in Treponema denticola2019

    • Author(s)
      山下慶子, 国分栄仁 菊池有一郎, 齋藤淳 石原和幸
    • Organizer
      日本細菌学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Oral dysbiosis in patients with palmoplantar pustulosis2018

    • Author(s)
      Akiyama Y, Minabe M, Ishihara K, Takahashi S, Kouno M, Nomura T
    • Organizer
      International Association for Dental Resarch
    • Related Report
      2018 Research-status Report
  • [Presentation] Characterization of a novel DNA binding protein of Treponema denticola2018

    • Author(s)
      Kitamura Y, Yamashita K, Kikuchi Y, Fukasawa T, Saito A, Ishihara K
    • Organizer
      International Association for Dental Resarch
    • Related Report
      2018 Research-status Report
  • [Presentation] Investigation of adhesion of Porphyromonas gingivalis Hgp44 to Treponema denticola2018

    • Author(s)
      Yoshikawa K, Kita D, Kokubu E, Kikuchi Y, Imamura K, Yamashita K, Kitamura Y, Fukazawa T, Saito A, Ishihara K
    • Organizer
      International Association for Dental Resarch
    • Related Report
      2018 Research-status Report
  • [Presentation] 歯周炎のマイクロバイオーム解析2018

    • Author(s)
      石原和幸
    • Organizer
      歯科基礎医学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 歯周基本治療における歯肉縁下マイクロバイオーム解析2018

    • Author(s)
      門田枝里子, 菊池有一郎, 富田幸代, 齋藤淳, 石原和幸
    • Organizer
      歯科学報
    • Related Report
      2018 Research-status Report
  • [Presentation] Treponema denticolaの感染による細胞応答2018

    • Author(s)
      国分栄仁, 菊池有一郎, 柴山和子, 石原和幸
    • Organizer
      日本細菌学会
    • Related Report
      2018 Research-status Report
  • [Book] ヒトマイクロバイオーム Vol.22020

    • Author(s)
      石原和幸
    • Total Pages
      624
    • Publisher
      (株)エヌ・ティー・エス
    • Related Report
      2020 Annual Research Report
  • [Book] 歯科医師・研究者チームによる 歯周治療のコンセンサス・1 歯周炎の新分類(2017)2020

    • Author(s)
      石原和幸 (分担)
    • Total Pages
      64
    • Publisher
      インターアクション
    • ISBN
      9784909066251
    • Related Report
      2019 Research-status Report
  • [Book] 根面う蝕の臨床戦略2018

    • Author(s)
      杉原直樹、高柳篤史、石原和幸、遠藤眞美、大鶴 洋、久保至誠、佐藤秀一、鈴木誠太郎、福島正義、見明康雄、宮崎真至、桃井保子
    • Total Pages
      116
    • Publisher
      クインテッセンス出版
    • ISBN
      9784781206189
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi