| Project/Area Number |
18K09560
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57020:Oral pathobiological science-related
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 口腔レンサ球菌 / アドヘジン / 多様性 / バイオフィルム / 病原因子 / デンタルプラーク / 感染性心内膜炎 |
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| Outline of Final Research Achievements |
Mitis group streptococci are the early colonizers of oral biofilms. Hsa adhesin of Streptococcus gordonii DL1 has the strong binding ability to the terminal sialic acid groups of host glycoproteins via the binding region, NR2, which is important for the pathogenicity of this organism. In this study, we isolated 186 wild-type strains of oral streptococci from healthy volunteers, and indicated certain relationship between the expression of NR2 region and the sialic-acid binding activity, detected by anti-NR2 antibody and by hemagglutinating activity, respectively. We also showed that S. gordonii strains were more likely to contain Hsa adhesins, and that the deduced amino acid sequence of the NR2 region was more diverse than the other regions. These results provide new insight into the adhesion process of oral streptococci.
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| Academic Significance and Societal Importance of the Research Achievements |
口腔レンサ球菌は、う蝕と歯周病、さらには感染性心内膜炎など歯性感染症の原因となる口腔バイオフィルム（デンタルプラーク）の初期形成に関与しているため、この研究のような付着因子の多様性の解明は、初期口腔バイオフィルム形成に関わる分子間結合様式の解明に寄与し、新たなプラークコントロール法の開発およびデンタルプラークをサンプルとした口腔バイオフィルム形成能および感染性心内膜炎病原性を評価する手段として臨床応用できる可能性を示すことができる。
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