A study of the possibility of an aggravating factor for periodontal disease that excessive fructose intake in diabetes mellitus.

• Project/Area Number: 18K09609
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Nihon University
• Principal Investigator: TANABE Natsuko 日本大学, 歯学部, 准教授 (10409097)
• Co-Investigator(Kenkyū-buntansha): 鈴木 直人 日本大学, 歯学部, 教授 (10226532)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周病 / 糖尿病 / 骨芽細胞 / グルコーストランスポーター / 終末糖化産物 / GLUT4 / AGEs / COX2 / 炎症

Outline of Final Research Achievements

The purpose of this study was to elucidate the causal relationship and molecular mechanism of the exacerbation of periodontal disease by excessive fructose intake in diabetes mellitus using osteoblasts by cell and molecular biology. As a result, fructose did not affect the progression of inflammation by fructose LPS in a hyperglycemic state. However, AGEs produced by hyperglycemia or glucose in the blood may exacerbate LPS-induced inflammation, which is one of the causes of inflammation in periodontal disease. In addition, related experiments showed that atmospheric pressure and cold nitrogen plasma could suppress the inflammation exacerbated by these AGEs.

Academic Significance and Societal Importance of the Research Achievements

糖尿病は歯周病増悪のリスクファクターとして疫学的及び動物実験においてもその相関が示されている。しかしながら，細胞・分子生物学的には明確な相関を示した報告は少ない。本研究の成果は，骨芽細胞に歯周病の炎症が進行する原因の一つあるLPSとグルコースまたは終末糖化産物で共刺激すると炎症性サイトカインや炎症性生理活性物質が増加するというものである。これは、糖尿病による高血糖状態における歯周病による炎症の進行の1つのメカニズムを細胞生物学的に明らかにしたものであると考えている。

Research Products

• [Journal Article] Glucose transporter 4 mediates LPS-induced IL-6 production in osteoblasts under high glucose conditions (2020)
  • Author(s): Kato S, Tanabe N, Nagao M, Sekino J, Tomita K, Sakai M, Abe K, Suzuki N, Ueda K.
  • Journal Title: Journal of Oral Science Volume: 62 Issue: 4 Pages: 423-426
  • DOI: 10.2334/josnusd.20-0010
  • NAID: 130007908555
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed
• [Presentation] Advanced glycation end products promote the COX2 expression of osteoblasts (2020)
  • Author(s): Keiko Tomita, Natsuko Tanabe, Takashi Kato, Noriyuki Arai, Naoto Suzuki, Shuichi Sato
  • Organizer: American Academy of Periodontology
  • Int'l Joint Research
• [Presentation] Hyperglycemia affects the expression of inflammatory cytokine in osteoblasts derived by LPS. (2019)
  • Author(s): Shunichiro Kato, Natsuko Tanabe, Jumpei Sekino, Naoto Suzuki, Abe Kimiko, Kouichiro Ueda
  • Organizer: The 97th General Session of the IADR
  • Int'l Joint Research
• [Presentation] 糖尿病が骨芽細胞のLPS誘導性炎症性サイトカイン発現に及ぼす影響 (2018)
  • Author(s): 加藤駿一郎，田邉奈津子，昔農淳平，酒井真悠，阿部仁子，鈴木直人，植田耕一郎
  • Organizer: 第７０回 日本大学歯学会総会・学術大会