| Project/Area Number |
18K09630
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57040:Regenerative dentistry and dental engineering-related
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| Research Institution | Tsurumi Junior College |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | TGF-β1 / Er:YAGレーザー / 再生療法 / MMP / HSP / COX-2 / アポトーシス / Caspase-3 / caspase-3 / COX2 |
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| Outline of Final Research Achievements |
It was suggested that in periodontal ligament cells, MMP-2 is involved in hard tissue induction by controlling TGF-β1 with low-power irradiation of Er: YAG laser. Moreover, MMP-2 and -3 are involved in wound healing by controlling cell proliferation. It was also suggested that HSP90, COX-2, and Caspase-3 are closely related to apoptosis and cell proliferation caused by laser irradiation.
In dental pulp cells (PPU-7 cells), low-power irradiation of Er: YAG laser affected apoptosis in the same manner as periodontal ligament cells. In addition, gene expression by laser irradiation showed an increase in odontoblast differentiation markers, but a decrease in osteoblast and chondrocyte differentiation markers.
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| Academic Significance and Societal Importance of the Research Achievements |
近年歯科臨床において,Er:YAGレーザー治療が保険導入され,保存治療領域に多く利用されるようになってきた。Er:YAGレーザー治療は創傷治癒を促進することが知られているが,その作用機序は不明な点が多く,基本的メカニズムが解明されれば治癒および再生促進に,より有効な治療手段として認識される。そこで今回,細胞増殖能や分化を制御する重要な生理活性物質であるTGF-β1に着目し,歯根膜細胞,歯髄細胞を用いてEr:YAGレーザーの低出力照射におけるTGF-β1の動態を経時的に比較検討することで,その作用機序解明の手掛かりを得ることは学術的かつ社会的意義があると考えられる。
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