Development of treatment strategies for implant induced BRONJ in rats
| Project/Area Number |
18K09664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAJIMA Kazunori 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (40707246)
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| Co-Investigator(Kenkyū-buntansha) |
黒嶋 伸一郎 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (40443915)
住田 吉慶 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
澤瀬 隆 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80253681)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | インプラント治療 / 薬剤関連顎骨壊死 / マクロファージ / BRONJ / デンタルインプラント / インプラント / ビスフォスフォネート製剤 / QQ-MNC / 細胞治療 / ステロイド / 幹細胞 / 細胞移植 |
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| Outline of Final Research Achievements |
The aims of this study were; 1) to create a high-prevalence model of bisphosphonate-related osteonecrosis of the jaw (BRONJ)-like lesion in rats, and; 2) to establish treatment strategy for BRONJ around implants by transplantation of quality and quantity controlled-peripheral blood mononuclear cells (QQ-PBMNCs). A high-prevalence rat model of BRONJ-like lesion around implants were successfully created by bisphosphonate and steroid combination therapy with implant placement. Macrophage polarization was significantly changed in BRONJ-like lesions around implants. Moreover, it was found that systemic transplantation of QQ-PBMNCs significantly healed or ameliorated impaired wound healing around implants. Our data may contribute to the clarification of pathophysiology of and establishment of treatment strategy for BRONJ around dental implants.
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| Academic Significance and Societal Importance of the Research Achievements |
インプラントの治療は日本でも普及しつつあるが，特定の薬を使用する患者では，口の中の傷がいつまでも治らない薬剤関連顎骨壊死という病気が問題となっている．この病気は2003年に発見されたが，現在でもその原因が分かっていない．私たちはその原因を追究すべく動物でこの病気モデルを作成することに成功し，血液から採取した細胞を特殊条件で育てて体内へ戻すことで，この病気が良くなることが分かった．
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Phase 1 clinical study of cell therapy with effective-mononuclear cells (E-MNC) for radiogenic xerostomia (first-in-human study) (FIH study on E-MNC therapy for radiogenic xerostomia)2020
Author(s)
Sumita Y, Iwamoto N, Seki M, Yoshida T, Honma R, Iwatake M, Ohba S, Takashi I, Hotokezaka Y, Harada H, Kuroshima S, Nagai K, Asahara T, Atsushi Kawakam I, Asahina I
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Journal Title
Medicine (Baltimore)
Volume: 99
Issue: 26
Pages: e20788-e20788
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] A stable protocol for the fabrication of transplantable human oral mucosal epithelial cell sheets for clinical application.2020
Author(s)
Kasai Y, Takagi R, Kobayashi S, Owaki T, Yamaguchi N, Fukuda H, Sakai Y, Sumita Y, Kanai N, Isomoto H, Kanetaka K, Ohki T, Asahina I, Nagai K, Nakao K, Takeda N, Okano T, Eguchi S, Yamato M.
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Journal Title
Regen Ther.
Volume: 14
Pages: 87-94
DOI
Related Report
Peer Reviewed
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[Journal Article] Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands.2019
Author(s)
I T, Sumita Y, Yoshida T, Honma R, Iwatake M, Raudales JLM, Shizuno T, Kuroshima S, Masuda H, Seki M, Tran SD, Asahara T, Asahina I.
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Journal Title
Stem Cell Res Ther.
Volume: 10
Issue: 1
Pages: 304-304
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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