Development of a novel cellulose scaffold to potentiate the transplanted cells survival for bone regeneration
| Project/Area Number |
18K09680
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
加来 賢 新潟大学, 医歯学系, 准教授 (30547542)
魚島 勝美 新潟大学, 医歯学系, 教授 (50213400)
井田 貴子 新潟大学, 医歯学総合病院, 医員 (60790285)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Methylcellulose / Scaffolds / Mesenchymal Stem cells / Bone Regeneration / Mesenchymal stem cells / Bone regeneration / Tissue Regeneration / scaffolds / bone regeneration / cellulose / scaffold |
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| Outline of Final Research Achievements |
In this investigation, we used Methylcellulose (MC) to develop and optimize a novel scaffold material for bone cell transplantation. A series of MC scaffolds with different porosity, cross-link density, and size were fabricated. Results showed that Sodium Chloride (NaCl) has a great effect on the homogeneity and porosity of the scaffold in a dose-dependent manner. Crosslinking using carbonyldiimidazole (CDI) at different concentrations showed no significant changes in the scaffold’s characteristics. As a result of the lyophilization procedure, the thickness of the scaffold was significantly reduced; consequently, affecting the scaffold's structure and compromising the mechanical strength needed for tissue transplantation. Although the production of MC scaffolds was achieved, homogeneity between samples was rather difficult to obtain. Thus, further optimization is required for the production of viable cell transplantation scaffolds using methylcellulose.
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| Academic Significance and Societal Importance of the Research Achievements |
Increase in the life expectancy and diseases that causes bone loss, has shown an urgent need to improve and optimize bone graft treatment. Development of novel regenerative biomaterials such as Methylcellulose scaffolds, aims to improve our knowledge on the intrinsic mechanism of tissue repair.
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Report
(4 results)
Research Products
(4 results)
