Elucidation and therapeutic application of tumor microenvironmental control mechanism by exosomes secreted by high-grade oral cancer
| Project/Area Number |
18K09771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57060:Surgical dentistry-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
Yoshida Ryoji 熊本大学, 大学院生命科学研究部(医), 准教授 (10632458)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 口腔扁平上皮癌 / エクソソーム / 治療抵抗性 / 放射線耐性 / マイクロRNA / 口腔がん / 腫瘍微小環境 / アポトーシス / DNAダメージ / 口腔癌 / 癌幹細胞 |
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| Outline of Final Research Achievements |
The present study focused on radiation resistance of high-grade oral cancer, especially oral squamous cell carcinoma (OSCC) cells. The exosomes secreted by the radiation-resistant OSCC cell line (SAS-R) established from the OSCC cell line SAS confer radioresistant phenotype on SAS and HSC2 through BAK regulation by the contained miR-503-3p and apoptosis suppression . In addition, as a result of analysis using patient's serum, it was clarified that the expression of miR-503-3p in blood correlates with the pathological response to chemoradiotherapy and the patient prognosis. The mechanism of radiation resistance acquisition was clarified
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| Academic Significance and Societal Importance of the Research Achievements |
放射線抵抗性は口腔がんの治療上、問題となるがん細胞の特性の一つである。本研究では放射線耐性口腔がん細胞がどのように周囲細胞に放射線抵抗性を与えるかを実験的に解析した。本研究から、放射線耐性口腔がん細胞はエクソソーム内に含まれるマイクロRNA（miR-503-3p）を介して放射線によって惹起される細胞死を抑制していることが解明された。また、血中miR-503-3pが放射線治療効果や口腔がんの予後と相関していることが明らかとなった。本研究成果は、新たな口腔がんの放射線耐性獲得機構の存在を示唆するものであり、将来の新規診断・治療法につながると考えられる。
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study2019
Author(s)
Nakashima H, Yoshida R, Hirosue A, Kawahara K, Sakata J, Arita H, Yamamoto T, Toya R, Murakami R, Hiraki A, Shinohara M, Ito T, Kuwahara Y, Nakayama H
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Journal Title
Tumour Biol
Volume: 41(3)
Issue: 3
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Exosomes from radioresistant cells enhances radioresistance phenotype in oral squamous cell carcinoma2019
Author(s)
Keisuke Yamana, Ryoji Yoshida, Hikaru Nakashima, Yuichiro Matsuoka, Hidetaka Arita, Junki Sakata, Sho Kawaguchi, Shunsuke Gohara, Yuka Nagao, Kenta Kawahara, Akiyuki Hirosue, Hideki Nakayama
Organizer
第78回 日本癌学会学術総会
Related Report
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