slc39a-dependent regulation for EMT of OSCC
| Project/Area Number |
18K09813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57060:Surgical dentistry-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
Hiroshi Shimasue 広島大学, 医系科学研究科(歯), 専門研究員 (40335683)
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| Co-Investigator(Kenkyū-buntansha) |
飛梅 圭 広島大学, 医系科学研究科(歯), 准教授 (40350037)
東川 晃一郎 広島大学, 病院(歯), 講師 (80363084)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | EMT / OSCC / SLC39 family / Zn transporter / 口腔癌 / Snail / ZIP / LIV1 / ZIP2 / 浸潤・転移 |
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| Outline of Final Research Achievements |
EMT by forced Snail expression, which was initially assumed to fix the mesenchymal trait in one direction, maintained reversibility while Snail was constitutively expressed.Furthermore, we succeeded in selecting and maintaining MET-resistant clones from Snail-expressing OM1. This enables gene expression profiling of three groups of epithelial trait retention, EMT-MET trait retention, and EMT trait retention in the OM1 genome background.
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| Academic Significance and Societal Importance of the Research Achievements |
本計画で確立できた、OM1のゲノムバックグラウンドで上皮形質のみ保持、EMT-MET形質保持、EMT形質保持の3群の遺伝子発現プロファイルはRNAseq法で得た膨大なデータであり、研究データベースとして世界的に活用が可能である。
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties2021
Author(s)
R Uetsuki, K Higashikawa, S Okuda, N Yamakado, F Ishida, A Rizqiawan, S Ono, M Takechi, K Mizuta, H Shigeishi, N Kamata, K Tobiume
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Journal Title
Biochemistry and Biophysics Reports
Volume: in press
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Immune response to cytosolic DNA via intercellular receptor modulation in oral keratinocytes and fibroblasts.2020
Author(s)
Naruse T, Ohta K, Kato H, Ishida Y, Shigeishi H, Sakuma M, Fukui A, Nakagawa T, Tobiume K, Nishi H, Takechi M.
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Journal Title
Oral Dis.
Volume: in press
Related Report
Peer Reviewed
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