• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A novel signaling eregulates patterning and growth in the face.

Research Project

Project/Area Number 18K09841
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57070:Developmental dentistry-related
Research InstitutionNara Medical University

Principal Investigator

Kawakami Masayoshi  奈良県立医科大学, 医学部, 学内講師 (20244717)

Co-Investigator(Kenkyū-buntansha) 和中 明生  奈良県立医科大学, 医学部, 教授 (90210989)
下村 忠弘  奈良県立医科大学, 医学部附属病院, 研究員 (90645607)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords発生・分化 / 細胞・遺伝子 / シグナル伝達 / 歯学 / 成長・発育 / 細胞・組織 / 上顎 / 遺伝子 / 歯科矯正学 / 細胞分化・組織形成
Outline of Final Research Achievements

Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal
prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results from the failure of the Wnt signaling pathway. We applied beads soaked with Dickkopf-1 (Dkk-1), Alsterpaullone (AL) or Wnt3a to the right side of the maxillary prominence of the chick embryo. The embryo showed a defect of the maxilla on the treated side, and skeletal staining revealed hypoplasia of the premaxilla and palatine bone as a result of Dkk-1-soaked bead implantation. Maxillary prominence treated with Dkk-1, which indicated that the deformity of the maxillary bone was controlled by gene targets of the Wnt signaling pathway. Wnt signaling might regulate N-cadherin expression via Msx1, resuliting in cell aggregation for osteochondrogenesis.

Academic Significance and Societal Importance of the Research Achievements

われわれは、これまで顎顔面の形成過程を司る分子シグナル機構を調べてきた。顔面の形成は胎生期、顔面突起が前下方に成長し、上顎突起と前頭鼻突起が癒合し(EMT)上口唇が形成される。唇顎裂発症においてはEMTが作用していないのではとの仮説を立て、今年度はEMTに作用する遺伝子シグナル作用について調べた。Dkk-1を用いてWNTシグナルを阻害するとEMTに作用する遺伝子の発現が減少し、唇顎裂を生じることが明らかとなった。したがって、すなわち口唇裂発症の原因はWNTシグナルをはじめとする顎顔面の形成過程を司る分子シグナル伝達がうまく行われていなかったことが考えられる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (25 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (7 results) Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 6 results) Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Int'l Joint Research] Life Science Institute/Life Science Institute/The University of British Columbia(カナダ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Oral Science Research Centre/College of Dentistry, Yonsei University(韓国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Life Sciences institute/The University of British Columbia(カナダ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] College of Dentistry, Yonsei University/Oral Science Research Centre(韓国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] The University of British Columbia/Life Science Institute(カナダ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of British Columbia/Life Sciences Institute(カナダ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] College of Dentistry, Yonsei University/Oral Science Research Centre(韓国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain2021

    • Author(s)
      Kouko Tatsumi, Kaoru Kinugawa, Ayami Isonishi, Masahiro Kitabatake, Hiroaki Okuda, Shoko Takemura, Tatsuhide Tanaka, Eiichiro Mori & Akio Wanaka
    • Journal Title

      Molecular Brain

      Volume: 14 Issue: 1 Pages: 1-12

    • DOI

      10.1186/s13041-021-00874-8

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Large-scale election microscopic volume imaging of interscicular oligodendrocytes in the mouse corpus callosum2021

    • Author(s)
      Tanak T, Ohno N, Osanai Y, Saitoh S, Thai TQ, Nishimura K, Shinjo T, Takemura S, Tatsumi K, Wanaka A.
    • Journal Title

      Glia

      Volume: 69 Issue: 10 Pages: 2488-2502

    • DOI

      10.1002/glia.24055

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.2021

    • Author(s)
      Nishimura K, Tanaka T, Takemura S, Tatsumi K, Wanaka A.
    • Journal Title

      Plos One

      Volume: 16 Issue: 3 Pages: e0247840-e0247840

    • DOI

      10.1371/journal.pone.0247840

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circadian rhythms of sorting nexin 25 in the mouse supra chiasmatic nucleus2020

    • Author(s)
      Takemura S, Nagano M, Isonishi A, Tanaka T, Tatsumi K, Yamano M, Minami Y, Shigeyoshi Y, Wanaka A.
    • Journal Title

      Neurosci Letters

      Volume: 727 Pages: 134897-134897

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Circadian rhythms of soting nexin 25 in the mouse suprachiasmatic nucleus2020

    • Author(s)
      Takemura S, Nagano M, Isonishi A, Tanaka T, Tatsumi K, Yamano M, Minami Y, Shigeyoshi Y, Wanaka A
    • Journal Title

      Neuroscience Letter

      Volume: 727 Pages: 134897-134897

    • DOI

      10.1016/j.neulet.2020.134897

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo2019

    • Author(s)
      Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Kirita T, Wanaka A
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 52 Issue: 1 Pages: 19-26

    • DOI

      10.1267/ahc.18038

    • NAID

      130007605401

    • ISSN
      0044-5991, 1347-5800
    • Year and Date
      2019-02-28
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice2019

    • Author(s)
      Ito Taeko、Tatsumi Kouko、Takimoto Yasumitsu、Nishimura Tadashi、Imai Takao、Yamanaka Toshiaki、Takeda Noriaki、Wanaka Akio、Kitahara Tadashi
    • Journal Title

      Brain Sciences

      Volume: 9 Issue: 11 Pages: 329-329

    • DOI

      10.3390/brainsci9110329

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Blood-to-brain communication in the hypothalamus for energy intake regulation.2019

    • Author(s)
      Morita-Takemura S, Wanaka A.
    • Journal Title

      Neurochem Int.

      Volume: 128 Pages: 135-142

    • DOI

      10.1016/j.neuint.2019.04.007

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Orthodontic management of patient with Marfan syndrome - case report and review of the literature.2019

    • Author(s)
      Kawakami M, Shimomura T, Kirita T
    • Journal Title

      International Journal of Dentistry and Oral Science

      Volume: 6 Pages: 676-680

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Olig2-Lineage Astrocytes: A Distinct Subtype of Astrocytes That Differs from GFAP Astrocytes2018

    • Author(s)
      Tatsumi Kouko、Isonishi Ayami、Yamasaki Miwako、Kawabe Yoshie、Morita-Takemura Shoko、Nakahara Kazuki、Terada Yuki、Shinjo Takeaki、Okuda Hiroaki、Tanaka Tatsuhide、Wanaka Akio
    • Journal Title

      Front. Neuroanat.

      Volume: 12 Pages: 1-17

    • DOI

      10.3389/fnana.2018.00008

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The inner mitochondrial membrane protein ANT1 modulates IL-6 expression via the JNK pathway in macrophages.2018

    • Author(s)
      Nakahara K, Tanaka T, Okuda H, Isonishi A, Morita-Takemura S, Tatsumi K, Wanaka A.
    • Journal Title

      FEBS Letters

      Volume: 592 Pages: 3750-3758

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] NGF and BDNF expression in mouse DRG after spared nerve injury.2018

    • Author(s)
      Terada Y, Morita-Takemura S, Isonishi A, Tanaka T, Okuda H, Tatsumi K, Shinjo T, Kawakguchi M, Wanaka A.
    • Journal Title

      Neuroscience Letters

      Volume: 686 Pages: 67-73

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 顔面の形態形成はJNK/WNTシグナルによって制御されている2022

    • Author(s)
      川上正良、下村忠弘、田中達英、和中明生、桐田忠昭
    • Organizer
      第81回日本矯正歯科学会学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Wnt Signaling Pathway Controls Epithelial-Mechythmal Transition in Upper Jaw development ofChick Embryo2020

    • Author(s)
      Kawakami M, Shimomura T,Tatsumi K,Tanaka T,Morita-Takemura S, Wanaka A,Kirita T
    • Organizer
      The 9th International Orthodontic Congress
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Wnt signaling Pathway Targete Developmental Control Genes During craniofacial Morphogenesis.2019

    • Author(s)
      Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Wanaka A, Kirita T.
    • Organizer
      97th IADR General session & Exhibition, Vancouver BC, Canada
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 顎顔面の形態発生時の上皮間葉移行に与えるWntシグナルの関与2019

    • Author(s)
      下村忠弘,川上正良,辰巳晃子、和中明生、桐田忠昭
    • Organizer
      第78回 日本矯正歯科学会学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Wnt Signaling Pathway Targets Developmental Control Genes During Craniofacial Morphogenesis.2019

    • Author(s)
      Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Wanaka A, Kirita T.
    • Organizer
      97th IADR General Session & Exhibition, Vancouver BC, CANADA
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Wnt/b-cateninシグナルがコントロールする顎顔面の形態形成パターン2018

    • Author(s)
      下村忠弘、川上正良、辰巳晃子、和中明生、桐田忠昭
    • Organizer
      第77回日本矯正歯科学会大会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi