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the analysis of relationship between fatty acids and bone metabolism in free fatty acid receptor GPR120 knockout mice.

Research Project

Project/Area Number 18K09850
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57070:Developmental dentistry-related
Research InstitutionTohoku University

Principal Investigator

Kimura Keisuke  東北大学, 歯学研究科, 大学院非常勤講師 (70712909)

Co-Investigator(Kenkyū-buntansha) 杉澤 晴紀  東北大学, 歯学研究科, 大学院非常勤講師 (20792162)
北浦 英樹  東北大学, 歯学研究科, 准教授 (60295087)
石田 匡彦  東北大学, 歯学研究科, 大学院非常勤講師 (80770891)
岸川 明子  東北大学, 大学病院, 医員 (10827273)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsGPR120 / DHA / 破骨細胞 / 骨芽細胞 / 肥満 / 歯の移動 / TNF-α / LPS / osteoclast / Docosahexaenoic acid / 遊離脂肪酸受容体GPR120 / 骨代謝
Outline of Final Research Achievements

In vitro, RANKL and TNF-α-induced osteoclast formation of wild-type mice was suppressed by DHA in vitro, but not inhibited that of GPR120 knockout mice. LPS was administered to supracalvariae for 5 days and osteoclast formation was examined. Osteoclast formation was suppressed in wild-type mice when DHA was co-administered, but not observed in GPR120 knockout mice. Therefore, it was clarified that LPS-induced osteoclast formation in vivo was suppressed by GPR120 stimulation. In addition, DHA was administered to a model of orthodontic tooth movement mouse model in wild-type mice. DHA had suppressed tooth movement. Furthermore, this inhibitory effect could not be confirmed in GPR120 knockout mice. These results suggested that orthodontic tooth movement is suppressed by DHA via GPR120 signaling.

Academic Significance and Societal Importance of the Research Achievements

近年、生活習慣病である肥満および肥満に随伴する脂肪肝、糖尿病など代謝異常が問題視されている。最近の研究でGPR120遺伝子が欠損したマウスで肥満や脂肪肝などが発生し、このGPR120が肥満に関与していることが発見された。また高年齢化に伴い、骨粗鬆症など骨代謝が問題となってきている。肥満と骨折の関係を調べた報告では、肥満の人は骨量が減少し、骨折の割合が増加することが報告されている。しかしながら、肥満と骨代謝の関係を調べた報告は少ない。これらのことから骨芽細胞形成および破骨細胞形成に対するGPR120の影響を調べることで骨代謝に対する肥満の影響を解明できると考え研究を行なった。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2019 2018

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Docosahexaenoic Acid Inhibits Inflammation-Induced Osteoclast Formation and Bone Resorption in vivoThrough GPR120 by Inhibiting TNF-α Production in Macrophages and Directly Inhibiting Osteoclast Formation2019

    • Author(s)
      Akiko Kishikawa, Hideki Kitaura, Keisuke Kimura, Saika Ogawa, Jiawei Qi, Wei-Ren Shen, Fumitoshi Ohori, Takahiro Noguchi, Aseel Marahleh, Yasuhiko Nara, Atsuhiko Ichimura, Itaru Mizoguchi
    • Journal Title

      frontier in endocrinology

      Volume: Volume 10 Pages: 157-157

    • DOI

      10.3389/fendo.2019.00157

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] G Protein-Coupled Receptor 120 signaling inhibited osteoclast formation and bone resorption.2018

    • Author(s)
      Akiko Kishikawa, Hideki Kitaura, Keisuke Kimura, Masahiko Ishida, Kazuhiro Shima, Saika Ogawa, Jiawei Qi, Wei-Ren Shen, Fumitoshi Ohori, Takahiro Noguchi, Aseel Marahleh, Itaru Mizoguchi
    • Organizer
      American Society for Bone and Mineral Research 2018 annual meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 破骨細胞形成、骨吸収および破骨細胞関連サイトカインの発現におけるDHAの影響についての検討2018

    • Author(s)
      岸川明子、木村桂介、石田匡彦、島和弘、小川紗衣香、セイカイ;、沈威任、大堀文俊、野口隆弘、Aseel Marahleh、北浦英樹
    • Organizer
      第77回日本矯正歯科学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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