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Use of a mid-infrared free-electron laser to treat infectious diseases: Its bactericidal effects against gram-negative bacteria

Research Project

Project/Area Number 18K09895
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57080:Social dentistry-related
Research InstitutionKanagawa Dental College

Principal Investigator

Toyama Toshizo  神奈川歯科大学, 歯学部, 特任講師 (60638906)

Co-Investigator(Kenkyū-buntansha) 吉田 彩佳  神奈川歯科大学, 歯学部, 准教授 (00609414)
築山 光一  東京理科大学, 理学部第一部化学科, 教授 (20188519)
浜田 信城  神奈川歯科大学, 歯学部, 教授 (20247315)
吉野 文彦  神奈川歯科大学, 歯学部, 准教授 (20308307)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords赤外自由電子レーザー / Porphyromonas gingivalis / Escherichia coli / aPDT / 歯周病 / 歯周病原因子 / LPS
Outline of Final Research Achievements

This study is based on the bactericidal effect of FEL on periodontopathic bacteria such as Porphyromonas gingivalis and Escherichia coli as endemic Gram-negative bacteria, and the effect of infrared free electron laser on periodontopathic bacteria was evaluated by analyzing the infrared absorption wavelengths of various bacteria and irradiating them with various wavelengths determined. The results showed that the laser's bactericidal effect was significantly enhanced by irradiation at wavelengths up to 6.62micrometre. As a result, the antibacterial effect was observed at the wavelength of 6.62micrometre, which corresponds to the C-H stretching vibration mode. The 6.62micrometre irradiation group was the most superior to the other wavelengths in inhibiting bacterial growth.

Academic Significance and Societal Importance of the Research Achievements

赤外自由電子レーザー (FEL) は中赤外領域を発振領域とする波長可変のレーザーであり,その波長域は分子の指紋領域をほぼ全域網羅しているため,殺菌作用のみならず,LPSなど細菌特有な分子の不活化が期待できるが,歯周病治療への応用はされていない。本研究課題では,FEL が照射範囲にある組織に対して温度変化をおこすことなく,人体に為害作用が少ない光線力学療法が可能であることが示唆された。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (17 results)

All 2021 2020 2019 2018

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (10 results)

  • [Journal Article] Inhibitory effect of omega-3 fatty acids on alveolar bone resorption and osteoclast differentiation2020

    • Author(s)
      Ozaki Yu、Morozumi Toshiya、Watanabe Kiyoko、Toyama Toshizo、Sasaki Haruka、Sato Takenori、Yamamoto Yuko、To Masahiro、Inaba Keitaro、Tsukinoki Keiichi、Hamada Nobushiro、Minabe Masato
    • Journal Title

      Journal of Oral Science

      Volume: 62 Issue: 3 Pages: 298-302

    • DOI

      10.2334/josnusd.19-0267

    • NAID

      130007863825

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Purification and characterization of a fimbrial protein from <i>Porphyromonas salivosa</i> ATCC 494072019

    • Author(s)
      KOYATA Yasunori、WATANABE Kiyoko、TOYAMA Toshizo、SASAKI Haruka、HAMADA Nobushiro
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 6 Pages: 916-923

    • DOI

      10.1292/jvms.19-0067

    • NAID

      130007668558

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antimicrobial photodynamic therapy using a plaque disclosing solution on Streptococcus mutans2019

    • Author(s)
      Shiotsu-Ogura Y, Yoshida A, Kan P, Sasaki H, Toyama T, Izukuri K, Hamada N, Yoshino F
    • Journal Title

      Photodiagnosis and Photodynamic Therapy

      Volume: 26 Pages: 252-257

    • DOI

      10.1016/j.pdpdt.2019.04.003

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Porphyromonas salivosa ATCC 49407 fimbriac induced osteoclast differentiation and cytokine production.2019

    • Author(s)
      Inaba K, Koyata Y, Sasaki H, Toyama T, Hiramine H, Ogawa T, Watanabe K, Hamada N
    • Journal Title

      神奈川歯学

      Volume: 54(2) Pages: 92-101

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ameliorating effects of Jixueteng in a mouse model of Porphyromonas gingivalis-induced periodontitis: Analysis based on gingival microcirculatory system.2019

    • Author(s)
      Suzuki M, Toyama T, Watanabe K, Sasaki H, Sugiyama S, Yoshino F, Yoshida A, Takahashi S-S, Wada-Takahashi S, Matsuo M, Todoki K, HamadaN
    • Journal Title

      Natural Product Communications

      Volume: 13(12) Issue: 12 Pages: 1699-1703

    • DOI

      10.1177/1934578x1801301230

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kampo Therapies and the Use of Herbal Medicines in the Dentistry in Japan2019

    • Author(s)
      Watanabe Shuji、Toyama Toshizo、Sato Takenori、Suzuki Mitsuo、Morozumi Akira、Sakagami Hiroshi、Hamada Nobushiro
    • Journal Title

      Medicines

      Volume: 6 Issue: 1 Pages: 34-34

    • DOI

      10.3390/medicines6010034

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of French Pine Bark Extract Chewing Gum on Oral Malodor and Salivary Bacteria2018

    • Author(s)
      WATANABE Kiyoko、HIRAMINE Hiroko、TOYAMA Toshizo、HAMADA Nobushiro
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 64 Issue: 3 Pages: 185-191

    • DOI

      10.3177/jnsv.64.185

    • NAID

      130007413266

    • ISSN
      0301-4800, 1881-7742
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The changes of gingival vascular network using Jixueteng in a mouse periodontitis model2021

    • Author(s)
      遠山歳三, 鈴木光雄, 松尾雅斗, 渡辺清子, 吉野文彦, 吉田彩佳, 高橋聡子, 高橋俊介, 浜田信城
    • Organizer
      第46回日本微小循環学会総会
    • Related Report
      2021 Annual Research Report
  • [Presentation] The changes of gingival vascular network using Jixueteng in a mouse periodontitis model2021

    • Author(s)
      Toshizo Toyama, Mitsuo Suzuki, Masato Matsuo, Kiyoko Watanabe, Fumihiko Yoshino, Ayaka Yoshida, Satoko Wada-Takahashi, Shun-suke Takahashi, Nobushiro Hamada
    • Organizer
      第46回日本微小循環学会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] Porphyromonas gingivalis感染による脳卒中易発症性高血圧自然発症ラット(SHRSP)における歯肉循環変化の解析2019

    • Author(s)
      高橋聡子,吉野文彦,吉田彩佳,前畑洋次郎,遠山歳三,佐藤武則,浜田信城,李昌一,高橋俊介
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Porphyromonas gingivalis含有色素を応用した新規抗菌光線力学療法の検討2019

    • Author(s)
      吉田彩佳,佐々木悠,居作和人,遠山歳三,浜田信城,吉野文彦
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ラット口腔組織に対する青色 LED 光照射の酸化ストレス惹起とその防御2018

    • Author(s)
      吉田 彩佳,遠山 歳三,吉野 文彦
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 赤外自由電子レーザーの齲蝕原性細菌に対する殺菌効果の検討2018

    • Author(s)
      遠山 歳三,渡辺 清子,吉野 文彦,吉田 彩佳,佐々木 悠,浜田 信城
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 歯周病原細菌内色素を利用した新規抗菌光線力学療法の検討2018

    • Author(s)
      吉田彩佳,小椋有香子,高橋俊介,高橋聡子,日髙弘一,杉山秀太,前畑洋次郎,宮本千央,堀 紀雄,遠山歳三,秦 光潤,前谷崇志,李 昌一,岡田永三,岡田康江,吉野文彦
    • Organizer
      第 18 回日本抗加齢医学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Porphyromonas gingivalis 感染による脳卒中易発症性高血圧ラット (SHRSP) の口腔内微小循環と大動脈血管機能の修飾2018

    • Author(s)
      日髙弘一,舩木誠子,徳冨文彬,高橋聡子,吉野文彦,吉田彩佳,前畑洋次郎,宮本千央,遠山歳三,佐藤武則,杉山秀太,浜田信城,李 昌一,高橋俊介
    • Organizer
      第 18 回日本抗加齢医学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 歯垢染色液への青色光照射を応用したう蝕病原細菌に対する影響2018

    • Author(s)
      小椋有香子,吉田彩佳,高橋俊介,高橋聡子,日髙弘一,杉山秀太,前畑洋次郎,宮本千央,堀 紀雄,遠山歳三,秦 光潤,前谷崇志,李 昌一,岡田永三,岡田康江,吉野文彦
    • Organizer
      第 18 回日本抗加齢医学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Porphyromonas salivosa線毛は破骨細胞分化とサイトカイン産生を誘導する2018

    • Author(s)
      18.稲葉啓太郎,佐々木悠,遠山歳三,佐藤武則,渡辺清子,合田征司,浜田信城
    • Organizer
      神奈川歯科大学学会第157回例会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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