Exploring the influence of oral bacteria on influenza virus infection

• Project/Area Number: 18K09920
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57080:Social dentistry-related
• Research Institution: Nihon University
• Principal Investigator: KAMIO Noriaki 日本大学, 歯学部, 准教授 (60546472)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: インフルエンザウイルス / 口腔細菌 / 歯周病原菌

Outline of Final Research Achievements

Fusobacterium nucleatum and Porphyromonas gingivalis are known to be involved in periodontal disease. In this study, we explored the effect of F. nucleatum on influenza virus infection. Our findings revealed that F. nucleatum enhanced influenza virus replication possibly attributable to F. nucleatum-reduced HDAC1 and HDAC6 expression. We also found that P. gingivalis enhanced pneumococcal adhesion to respiratory cells by inducing PAFR expression. These results provides the scientific basis to support the importance of keeping good oral hygiene as an effective approach in preventing the occurrence and/or progression of respiratory infection.

Academic Significance and Societal Importance of the Research Achievements

本研究により、口腔細菌、とくに歯周病原菌がインフルエンザウイルス感染や肺炎球菌感染の促進に働いていることが示唆された。そのため、口腔を健康に保つことが、インフルエンザウイルス感染の予防につながる可能性がある。さらに、インフルエンザ罹患後にしばしば細菌性肺炎を合併し重症化することがあることから、重症化を予防する上でも健康な口腔は重要と考えられる。以上のことから、本研究は、口腔環境の管理による呼吸器感染症予防法を確立する上で、科学的根拠を構築するための一助となったと考える。

Research Products

• [Journal Article] Aspiration of periodontopathic bacteria due to poor oral hygiene potentially contributes to the aggravation of COVID-19 (2021)
  • Author(s): Takahashi Y, Watanabe N, Kamio N, Kobayashi R, Iinuma T, Imai K
  • Journal Title: Journal of Oral Science Volume: 63 Issue: 1 Pages: 1-3
  • DOI: 10.2334/josnusd.20-0388
  • NAID: 130007959227
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed / Open Access
• [Journal Article] Expression of the SARS-CoV-2 Receptor ACE2 and Proinflammatory Cytokines Induced by the Periodontopathic Bacterium Fusobacterium nucleatum in Human Respiratory Epithelial Cells (2021)
  • Author(s): Takahashi Y, Watanabe N, Kamio N, Yokoe S, Suzuki R, Sato S, Iinuma T, Imai K
  • Journal Title: Int J Mol Sci. Volume: 22(3) Issue: 3 Pages: 1352-1352
  • DOI: 10.3390/ijms22031352
  • Peer Reviewed / Open Access
• [Journal Article] Porphyromonas gingivalis enhances pneumococcal adhesion to human alveolar epithelial cells by increasing expression of host platelet-activating factor receptor. (2021)
  • Author(s): Kamio N, Hayata M, Tamura M, Tanaka H, Imai K
  • Journal Title: FEBS Lett Volume: in press Issue: 11 Pages: 1604-1612
  • DOI: 10.1002/1873-3468.14084
  • Peer Reviewed / Open Access
• [Journal Article] Heat-Killed Fusobacterium nucleatum Triggers Varying Heme-Related Inflammatory and Stress Responses Depending on Primary Human Respiratory Epithelial Cell Type. (2020)
  • Author(s): Ryo Koike, Marni E Cueno, Keiko Nodomi, Muneaki Tamura, Noriaki Kamio, Hajime Tanaka, Ai Kotani, Kenichi Imai.
  • Journal Title: Molecules Volume: 25(17) Issue: 17 Pages: 3839-3839
  • DOI: 10.3390/molecules25173839
  • Peer Reviewed / Open Access
• [Journal Article] The periodontopathic bacterium Fusobacterium nucleatum induced proinflammatory cytokine production by human respiratory epithelial cell lines and in the lower respiratory organs in mice. (2019)
  • Author(s): Hayata M, Watanabe N, Tamura M, Kamio N, Tanaka H, Nodomi K, Miya C, Nakayama E, Ueda K, Ogata Y, Imai K
  • Journal Title: Cellular Physiology and Biochemistry Volume: 53 Issue: 1 Pages: 49-61
  • DOI: 10.33594/000000120
  • Peer Reviewed / Open Access
• [Journal Article] Cynaropicrin from Cynara scolymus L. suppresses Porphyromonas gingivalis LPS-induced production of inflammatory cytokines in human gingival fibroblasts and RANKL-induced osteoclast differentiation in RAW264.7 cells (2018)
  • Author(s): Hayata Mayumi、Watanabe Norihisa、Kamio Noriaki、Tamura Muneaki、Nodomi Keiko、Tanaka Kiyotaka、Iddamalgoda Arunasiri、Tsuda Hiromasa、Ogata Yorimasa、Sato Shuichi、Ueda Kouichiro、Imai Kenichi
  • Journal Title: Journal of Natural Medicines Volume: 73 Issue: 1 Pages: 114-123
  • DOI: 10.1007/s11418-018-1250-6
  • NAID: 40021790096
  • Peer Reviewed / Open Access
• [Journal Article] Ions released from a S-PRG filler induces oxidative stress in Candida albicans inhibiting its growth and pathogenicity (2018)
  • Author(s): Tamura Muneaki、Cueno Marni E.、Abe Kazumasa、Kamio Noriaki、Ochiai Kuniyasu、Imai Kenichi
  • Journal Title: Cell Stress and Chaperones Volume: 23 Issue: 6 Pages: 1337-1343
  • DOI: 10.1007/s12192-018-0922-1
  • Peer Reviewed
• [Journal Article] MiR-200b attenuates IL-6 production through IKKβ and ZEB1 in human gingival fibroblasts. (2018)
  • Author(s): Matsui S, Zhou L, Nakayama Y, Mezawa M, Kato A, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Ogata Y
  • Journal Title: Inflamm Res Volume: 67 Issue: 11-12 Pages: 965-973
  • DOI: 10.1007/s00011-018-1192-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 歯周病原菌Porphyromonas gingivalisがインフルエンザウイルス感染に及ぼす影響 (2018)
  • Author(s): 神尾 宜昌，田村 宗明，今井 健一
  • Organizer: 第92回日本感染症学会学術講演会
• [Presentation] 口腔細菌による肺炎発症メカニズム（１）－P. gingivalisはPAFR発現と肺炎レンサ球菌の肺細胞への付着を促進する－ (2018)
  • Author(s): 神尾 宜昌，田村 宗明，早田 真由美，渡辺 典久，宮 千尋，今井 健一
  • Organizer: 第60回日本老年医学会学術集会
• [Presentation] ジンジパインは呼吸器上皮細胞において肺炎球菌受容体PAFRの発現を誘導する (2018)
  • Author(s): 神尾 宜昌，早田 真由美，田村 宗明，今井 健一
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] 歯周病原菌Porphyromonas gingivalisはインフルエンザウイルスのヘマグルチニンを開裂し感染を促進する (2018)
  • Author(s): 神尾 宜昌，田村 宗明，今井 健一
  • Organizer: 第67回日本感染症学会東日本地方会学術集会