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Development of a new preventive method for root caries using lipid oxide and its application to fluoride combination therapy

Research Project

Project/Area Number 18K09926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57080:Social dentistry-related
Research InstitutionTsurumi University

Principal Investigator

Hanada Nobuhiro  鶴見大学, 歯学部, 教授 (70180916)

Co-Investigator(Kenkyū-buntansha) 村田 貴俊  鶴見大学, 歯学部, 講師 (10313529)
下田 信治  鶴見大学, 歯学部, 教授 (30139620)
岡田 彩子  鶴見大学, 歯学部, 助教 (60515584)
広田 一男  鶴見大学, 歯学部, 非常勤講師 (60563848)
野村 義明  鶴見大学, 歯学部, 学内教授 (90350587)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords酸化脂質 / LDL / HDL / 齲蝕 / 根面齲蝕 / 酸化LDL / 石灰化 / 歯石 / カルシウム沈着 / 象牙細管 / 根面う蝕 / コレステロール / カルシウム / リン酸カルシウム / 再石灰化 / LDLコレステロール / フッ化カルシウム / oxLDL / フッ化物 / 生活習慣病 / 歯周病
Outline of Final Research Achievements

Focusing on the oxidation of LDL in the gingival crevicular fluid due to gingivitis, we hypothesized that oxidized LDL (oxLDL) deposits supersaturated calcium on the tooth surface.
In this study, change in calcium deposition level were monitored for carbohydrates, lipids, and proteins. As a result, no promotion of calcium deposition was observed even when the concentration and molecular weight of various carbohydrates and proteins were changed. However, in the experimental system to which oxLDL was added, the promotion of calcium deposition was observed depending on the concentration of the added oxLDL. Electron microscopic findings also demonstrated the calcification ability of oxLDL. These data suggest that oxLDL may function as an anti-cariogenic preparation by a different mechanism from that of fluoride.

Academic Significance and Societal Importance of the Research Achievements

本研究により酸化 LDL (oxLDL) のカルシウム沈着能力が示された。歯肉溝の LDLが慢性炎症によりoxLDLに変化することで、脱灰歯面の再石灰化が促進される。このことは、oxLDLがフッ化物とは異なる機序の抗齲蝕製剤として機能する可能性を意味している。しかし、同時に歯肉溝においてoxLDLの働きで歯石が形成される可能性がある。従来から、歯周病と糖尿病や内臓脂肪症候群の関連性が示唆されている。本研究により歯科診療所で糖尿病や内臓脂肪症候群を積極的に予防し、血液や歯肉溝の LDLやoxLDLの濃度を常時抑えることが歯周病の予防戦略として必要であることが明らかになった。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (19 results)

All 2021 2020 2019 2018

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 14 results,  Open Access: 13 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] Dental Drug Delivery System を導入した 保健指導プロトコルの有効性を検討する:予備的臨床試験2021

    • Author(s)
      岡田彩子、村田貴俊、大塚良子、曽我部薫、 有吉芽生、マティン カイルール、花田信弘
    • Journal Title

      口腔衛生学会雑誌

      Volume: 71

    • NAID

      130008043232

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oxidized Low-Density Lipoprotein Promotes In Vitro Calcification2020

    • Author(s)
      Yamashita Mamiko、Nomura Yoshiaki、Ishikawa Misao、Shimoda Shinji、Hanada Nobuhiro
    • Journal Title

      Materials

      Volume: 13 Issue: 22 Pages: 5120-5120

    • DOI

      10.3390/ma13225120

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Risk factors for postoperative pneumonia according to examination findings before surgery under general anesthesia2020

    • Author(s)
      Yuko Inai , Yoshiaki Nomura , Tohru Takarada , Nobuhiro Hanada , Naohisa Wada
    • Journal Title

      Clinical oral investigations

      Volume: Epub ahead of print Issue: 10 Pages: 3577-3585

    • DOI

      10.1007/s00784-020-03230-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of cariogenic bacteria and sealant evaluated by International Caries Detection Assessment System2019

    • Author(s)
      Nomura Y, Fujita Y, Ishihara Y, Kakuta E, Okada A, Maki K, Hanada N
    • Journal Title

      Open Dent J

      Volume: 13 Issue: 1 Pages: 512-519

    • DOI

      10.2174/1874210601913010512

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] PRECEDE-PROCEED model based questionnaire and saliva tests for oral health checkup in adult2019

    • Author(s)
      Nomura Y , Matsuyama T , Fukai K , Okada A , Ida M , Yamauchi N , Hanamura H , Yabuki Y , Watanabe K , Sugawara M , Imanishi Y , Koizumi N , Murano Y , Nishiyama A , Fukukawa Y , Otsuka R , Hanada N
    • Journal Title

      Journal of Oral Science

      Volume: 61 Issue: 4 Pages: 544-548

    • DOI

      10.2334/josnusd.18-0288

    • NAID

      130007751518

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Smoking and periodontal microorganisms2019

    • Author(s)
      Hanioka T, Morita M, Yamamoto T, Inagaki K, Wang P-L, Ito H, Morozumi T, Takeshita T, Suzuki N, Shigeishi H, Sugiyama M, Ohta K, Nagao T, Hanada N, Ojima M, Ogawa H
    • Journal Title

      The Japanese Dental Science Review

      Volume: 55 Issue: 1 Pages: 88-94

    • DOI

      10.1016/j.jdsr.2019.03.002

    • NAID

      50014414225

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Purification of a High Molecular Mass Protein in Streptococcus mutans.2019

    • Author(s)
      Murata T , Yamashita M , Ishikawa M , Shibuya K , Hanada N
    • Journal Title

      Journal of visualized experiments : JoVE

      Volume: 151 Issue: 151 Pages: 151-151

    • DOI

      10.3791/59804

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Consistency of supplied food and dentition status of the elderly in residential care homes.2019

    • Author(s)
      Nomura Y , Okada A , Kakuta E , Otsuka R , Sogabe K , Yamane K , Yamamoto T , Shigeta Y , Shigemoto S , Ogawa T , Hanada N
    • Journal Title

      BMC Oral Health

      Volume: 19 Issue: 1 Pages: 74-74

    • DOI

      10.1186/s12903-019-0770-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influences of Masticatory Function Recovery Combined with Health Guidance on Body Composition and Metabolic Parameters2019

    • Author(s)
      Takeuchi Hiroaki、Terada Mika、Kobayashi Kazuko、Uraguchi Masahide、Nomura Yoshiaki、Hanada Nobuhiro
    • Journal Title

      The Open Dentistry Journal

      Volume: 13 Issue: 1 Pages: 124-136

    • DOI

      10.2174/1874210601913010124

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevalence of Dental Caries in 5- and 6-Year-Old Myanmar Children.2019

    • Author(s)
      Nomura Y , Maung K , Kay Khine EM , Sint KM , Lin MP , Win Myint MK , Aung T , Sogabe K , Otsuka R , Okada A , Kakuta E , Wint WY , Uraguchi M , Hasegawa R , Hanada N
    • Journal Title

      International journal of dentistry

      Volume: 2019 Pages: 5948379-5948379

    • DOI

      10.1155/2019/5948379

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Corrigendum to "Prevalence of Dental Caries in 5- and 6-Year-Old Myanmar Children".2019

    • Author(s)
      Nomura Y , Maung K , Kay Khine EM , Myo Sint K , Phyo Lin M , Win Myint MK , Aung T , Sogabe K , Otsuka R , Okada A , Kakuta E , Yee Wint W , Uraguchi M , Hasegawa R , Hanada N
    • Journal Title

      International journal of dentistry

      Volume: 2019 Pages: 6986412-6986412

    • DOI

      10.1155/2019/6986412

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Feasibility Controlling Hemoglobin A1c by Oral Hygiene Improvement: A Pilot Study.2019

    • Author(s)
      Okada A , Nomura Y , Miyanohara M , Uraguchi M , Tadokoro H , Nagai T , Fujii Y , Miura M , Kawachi R , Matsui T , Takayanagi K , Yamamoto M , Yamamoto T , Hanada N
    • Journal Title

      Online Journal of Dentistry & Oral Health (OJDOH)

      Volume: April-2019 Pages: 8-15

    • DOI

      10.36811/ojdoh.2019.110003

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Method for functional analysis of a gene of interest in Streptococcus mutans: gene disruption followed by purification of a polyhistidine-tagged gene product.2018

    • Author(s)
      Murata T, Ishikawa M, Shibuya K, Hanada N.
    • Journal Title

      J Microbiol Methods.

      Volume: 155 Pages: 49-54

    • DOI

      10.1016/j.mimet.2018.11.010

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tooth loss and pneumonia mortality: A cohort study of Japanese dentists2018

    • Author(s)
      Suma S, Naito M, Wakai K, Naito T, Kojima M, Umemura O, Yokota M, Hanada N,Kawamura T
    • Journal Title

      PLoS One

      Volume: 13 Issue: 4 Pages: e0195813-e0195813

    • DOI

      10.1371/journal.pone.0195813

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 酸化LDLによる根面う蝕予防2020

    • Author(s)
      山下万美子, 野村義明 , 村田貴俊 , 岡田彩子 , 広田一男, 石川美佐緒, 下田信治, 花田信弘
    • Organizer
      第69回日本口腔衛生学会・総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 【腸内フローラとディスバイオーシス(バランス失調)】口腔微生物叢と歯のケアが腸内微生物叢に及ぼす影響2019

    • Author(s)
      花田 信弘 , 野村 義明 , 村田 貴俊 , 岡本 公彰
    • Organizer
      (公財)ヤクルト・バイオサイエンス研究財団: 腸内フローラシンポジウム
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Essential Role of S. mutans gtfC on Adherence to Glass Surface2018

    • Author(s)
      Murata T, Hanada N
    • Organizer
      96th General Session of the International Association for Dental Research (IADR)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] [臨床編] 3DSセラピーができる本2021

    • Author(s)
      花田信弘、宮之原真由、武内博朗、浦口昌秀、 高橋史典、平識善大、河内 亮、椎木健次、 西平守昭、三浦正洋、田所久永、長井哲弥、藤井佳人、波多江正樹、仲若三男、長谷川了
    • Total Pages
      144
    • Publisher
      第一歯科出版
    • ISBN
      9784924858770
    • Related Report
      2020 Annual Research Report
  • [Book] 臨床歯科栄養学~歯科に求められる栄養の基礎知識~2018

    • Author(s)
      花田信弘、萩原芳幸、北川昇
    • Total Pages
      148
    • Publisher
      一般財団法人 口腔保健協会
    • ISBN
      9784896053494
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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