| Project/Area Number |
18K10040
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 58020:Hygiene and public health-related: including laboratory approach
|
|---|
| Research Institution | Osaka Institute of Public Health |
|---|
Principal Investigator |
Kawahara Ryuji 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 主幹研究員 (10332454)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
中村 寛海 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 主幹研究員 (00332445)
|
|---|
| Project Period (FY) |
2018-04-01 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 薬剤耐性菌 / カルバペネム耐性腸内細菌 / CRE / プラスミド / ゲノム解析 / IMP-6 / 薬剤耐性プラスミド / カルバペネマーゼ / カルバペネム耐性腸内細菌科細菌 / 分子疫学 / NGS解析 |
|---|
| Outline of Final Research Achievements |
We determinated whether carbapenem-resistant Escherichia coli and Klebsiella pneumoniae carrying the IMP-6 gene, which are widely isolated in Osaka Prefecture, were associated with the huge outbreak at a hospital in Osaka, and whether plasmids containing the IMP-6 gene were involved as a factor in their spread. Comparison of the strains by PFGE and the full sequence of plasmids suggested that there was an association between the outbreak cases and the strains that spread in the region. In addition, one similar strain was detected in retail meat, and also related with the strains. Although the details of how these strains spread throughout the region are not known, it is suggested that the outbreak case contributed to the spread of drug-resistant bacteria in the region.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究の目的は、地域内の薬剤耐性菌がどのように広がっていったのか、その要因を明らかにすることである。薬剤耐性遺伝子を持つプラスミドは菌株から菌株への伝達経路として重要であると言われてきたが、実際に地域内での耐性菌拡大に寄与していたことを証明するのは困難であった。今回、全ゲノム解析という手法を用いてプラスミドを解析することで、薬剤耐性プラスミドの拡散が、地域内での薬剤耐性菌の蔓延の要因の一つであったことを示す結果を得ることができた。
|
