Molecular epidemiology of Staphylococcus aureus, MRSA having novel enterotoxins and virulence factors

• Project/Area Number: 18K10054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58020:Hygiene and public health-related: including laboratory approach
• Research Institution: Sapporo Medical University
• Principal Investigator: Aung Meiji Soe 札幌医科大学, 医学部, 講師 (10749584)
• Co-Investigator(Kenkyū-buntansha): 川口谷 充代 札幌医科大学, 医学部, 講師 (70733062) ; 小林 宣道 札幌医科大学, 医学部, 教授 (80186759) ; 漆原 範子 札幌医科大学, 医学部, 准教授 (80396308)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 黄色ブドウ球菌 / MRSA / S. argenteus / 新型エンテロトキシン / 病原因子 / 分子疫学 / ST / 新規病原因子 / 新規変異型付着因子 / 付着因子

Outline of Final Research Achievements

Prevalence of novel staphylococcal enterotoxin (SE)(-like) toxin genes in Staphylococcus aureus and its relative species S. argenteus, and their molecular epidemiological characteristics were analyzed. selw and selx were detected in more than 80% of S. aureus isolates examined, and showed diversity in their nucleotide sequences. S. aureus harbored sey and selz at low rate, without having sel26 and sel27. The sey gene was detected in all the ST2250 S. argenteus isolates, and more than half of this lineage harbored selz, sel26, and sel27. Prevalence of novel SE(-like) genes was revealed to be different depending on lineages of S. aureus and S. argenteus.

Academic Significance and Societal Importance of the Research Achievements

黄色ブドウ球菌は、市中での食中毒や病院感染の主要な原因菌である。本菌種の病原性は、本菌が持つ毒素、病原因子に起因すると考えられており、すでに腸管毒（エンテロトキシン、SE）、白血球障害毒素、組織付着因子などが同定されている。SE遺伝子にはsea-see、seg-seuなど多数の種類が知られるが、2011年以降、selw、selx、sey、selz、sel26、sel27などの新型SE（様）遺伝子が同定された。本研究ではそれらSE（様）遺伝子の黄色ブドウ球菌およびその近縁種における分布状況を明らかにするとともに、菌の遺伝学的系統によって分布するSE（様）遺伝子の種類に違いがあることを示した。

Research Products

• [Journal Article] Distribution of virulence factors and resistance determinants in three genotypes of Staphylococcus argenteus clinical isolates in Japan. (2021)
  • Author(s): Aung MS, Urushibara N, Kawaguchiya M, Hirose M, Ike M, Ito M, Kobayashi N.
  • Journal Title: Pathogens Volume: 10 Issue: 2 Pages: 163-163
  • DOI: 10.3390/pathogens10020163
  • Peer Reviewed / Open Access
• [Journal Article] Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan : identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec (2021)
  • Author(s): Aung MS, Urushibara N, Kawaguchiya M, Hirose M, Ito M, Habadera S, Kobayashi N
  • Journal Title: J Glob Antimicrob Resist Volume: 21 Pages: 207-214
  • DOI: 10.1016/j.jgar.2020.12.008
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence and Genetic Diversity of Staphylococcal Enterotoxin (-Like) Genes sey, selw, selx, selz, sel26 and sel27 in Community-Acquired Methicillin-Resistant Staphylococcus aureus. (2020)
  • Author(s): Aung MS, Urushibara N, Kawaguchiya M, Ito M, Habadera S, Kobayashi N.
  • Journal Title: Toxins (Basel) Volume: 12 Issue: 5 Pages: 347-347
  • DOI: 10.3390/toxins12050347
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Characterization of Methicillin-Susceptible and -Resistant Staphylococcus aureus Harboring Panton-Valentine Leukocidin-Encoding Bacteriophages in a Tertiary Care Hospital in Myanmar (2020)
  • Author(s): Aung MS, San T, Urushibara N, San N, Oo WM, Soe PE, Kyaw Y, Ko PM, Thu PP, Hlaing MS, Kawaguchiya M, Kobayashi N
  • Journal Title: Microbial Drug Resistance Volume: 25 Issue: 4 Pages: 360-367
  • DOI: 10.1089/mdr.2019.0208
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clonal Diversity and Genetic Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates From a Tertiary Care Hospital in Japan (2019)
  • Author(s): Aung MS, Urushibara N, Kawaguchiya M, Sumi A, Shinagawa M, Takahashi S, Kobayashi N
  • Journal Title: Microbial Drug Resistance Volume: 25 Issue: 8 Pages: 1164-1175
  • DOI: 10.1089/mdr.2018.0468
  • Peer Reviewed
• [Journal Article] Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV (2019)
  • Author(s): Aung MS, Urushibara N, Kawaguchiya M, Sumi A, Takahashi S, Ike M, Ito M, Habadera S, Kobayashi N
  • Journal Title: Microorganisms Volume: 7 Issue: 10 Pages: 389-389
  • DOI: 10.3390/microorganisms7100389
  • Peer Reviewed / Open Access
• [Journal Article] Molecular characterization of Staphylococcus argenteus in Myanmar: identification of novel genotypes/clusters in staphylocoagulase, protein A, alpha-haemolysin and other virulence factors. (2019)
  • Author(s): Aung MS, San T, San N, Oo WM, Ko PM, Thet KT, Urushibara N, Kawaguchiya M, Sumi A, Kobayashi N.
  • Journal Title: Journal of Medical Microbiology Volume: 68 Issue: 1 Pages: 95-104
  • DOI: 10.1099/jmm.0.000869
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular epidemiological characterization of methicillin-susceptible and -resistant Staphylococcus aureus isolated from skin and soft tissue infections in Bangladesh. (2019)
  • Author(s): Haque N, Aung MS, Paul SK, Bari MS, Ahmed S, Sarkar SR, Roy S, Nasreen SA, Mahmud MC, Hossain MA, Urushibara N, Kawaguchiya M, Sumi A, Kobayash N
  • Journal Title: Microbial Drug Resistance Volume: 25 Issue: 2 Pages: 241-250
  • DOI: 10.1089/mdr.2018.0123
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prevalence and genetic characteristics of Methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci isolated from oral cavity of healthy children in Japan. (2019)
  • Author(s): Hirose M, Aung MS, Fukuda A, Murata Y, Saitoh M, Kobayashi N.
  • Journal Title: Microbial Drug Resistance Volume: 25 Issue: 3 Pages: 400-407
  • DOI: 10.1089/mdr.2018.0333
  • Peer Reviewed
• [Presentation] 北日本の北海道におけるスタフィロコッカス・アルジェンテウスの分子疫学． (2021)
  • Author(s): アウン メイジソウ、漆原範子、川口谷充代、小林宣道．
  • Organizer: 第94回日本細菌学会総会
• [Presentation] 北海道における新規ブドウ球菌種Staphylococcus argenteusの分子疫学的解析． (2021)
  • Author(s): 小林宣道、アウン メイジソウ、川口谷充代、漆原範子、伊藤政彦、幅寺敏
  • Organizer: 第91回日本衛生学会総会
• [Presentation] 日本（北海道）におけるStaphylococcus argenteusの分子疫学と分子系統. (2020)
  • Author(s): Meijisoe Aung、漆原範子、川口谷充代、小林宣道
  • Organizer: 第93回日本細菌学会総会
• [Presentation] Genetic characterization of virulence factor genes in Syaphylococcus argenteus. (2019)
  • Author(s): Meiji Soe Aung、Thida San、漆原範子、川口谷充代、小林宣道
  • Organizer: 第92回日本細菌学会総会
• [Presentation] Molecular characterization of Staphylococcus aureus and Staphylococcus argenteus from food handlers in Myanmar. (2018)
  • Author(s): Meiji Soe Aung、漆原範子、Thida San、川口谷充代、小林宣道
  • Organizer: 第63回日本ブドウ球菌研究会