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Role of acetylation in alcoholic liver disease

Research Project

Project/Area Number 18K10130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 58040:Forensics medicine-related
Research InstitutionKumamoto University

Principal Investigator

Nishitani Yoko  熊本大学, 大学院生命科学研究部(医), 教授 (30359997)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアルコール / 初代培養肝細胞 / 細胞同調 / 概日リズム / 日内変動 / コラーゲン培地 / コラーゲンゲル培地 / アセチル化制御 / 脂質代謝 / 肝障害
Outline of Final Research Achievements

Ethanol affects the various intracellular signaling. Since ethanol metabolism via ADH (alcohol dehydrogenases) decreases NAD in hepatocyte, depletion of NAD may lead to insufficence of sirtuins that work as NAD-related deacetylation. We tried to establish the collagen-sandwich methods of primary rat hepatocyte culture, that is expected reflected more biological condition. However, collagen-sandwich methods was not suitable to treat the short-time changing. We decided to try the synchronization of cultured heptocyte via normal rat heptocyte culture. The synchronization was performed using 50% serum exposure, or high-dose insulin or dexametazone exposure and the samples were collect. We continue to analyze the mRNA expression.

Academic Significance and Societal Importance of the Research Achievements

本研究ではコラーゲンサンドイッチ法による初代培養肝細胞の可能性を提示した。しかしながら、実際にmRNAを抽出したりタンパク質を抽出しての短期での変化を検討するには困難な手法であると判断した。培養細胞の概日リズム同期については検討中であるが、末梢臓器でしかも細胞単位での概日リズムは生体内では重要であるが注目はされずらく、今後検討を重ねる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] High blood mirtazapine concentration in a newborn - A case of suspected portpartum infanticide2021

    • Author(s)
      Sasao A, Yonemitsu K, Ohtsu Y, Tsutsumi H, Furukawa S, Kimura-Mishima S, Nishitani Y
    • Journal Title

      Legal Medicine

      Volume: 48 Pages: 101830-101830

    • DOI

      10.1016/j.legalmed.2020.101830

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 発見日と場所が著しく異なった川への自動車転落事故同乗者の2剖検例2021

    • Author(s)
      中田拓冶, 大津由紀, 堤博志, 古川翔太, 笹尾亜子, 西谷陽子
    • Journal Title

      法医学の実際と研究

      Volume: 64 Pages: 153-157

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of arterial hemorrhage speed on the blood coagulation/fibrinolysis system and hemodynamics in rats.2020

    • Author(s)
      Furukawa S, Sasao A, Yonemitsu K, Ohtsu Y, Tsutsumi H, Taguchi K, Otagiri M, Nishitani Y.
    • Journal Title

      Blood Coagul Fibrinolysis.

      Volume: 31 Issue: 3 Pages: 198-206

    • DOI

      10.1097/mbc.0000000000000899

    • Related Report
      2019 Research-status Report
  • [Journal Article] Cerebrospinal fluid neurotransmitter levels and central nervous system depression in a rat drug overdose model2019

    • Author(s)
      Tsutsumi Hiroshi、Yonemitsu Kosei、Sasao Ako、Ohtsu Yuki、Furukawa Shota、Nishitani Yoko
    • Journal Title

      Toxicology Mechanisms and Methods

      Volume: 30 Issue: 2 Pages: 139-145

    • DOI

      10.1080/15376516.2019.1672122

    • Related Report
      2019 Research-status Report
  • [Presentation] 薬物検出に対する組換え抗体技術の応用2021

    • Author(s)
      笹尾亜子, 堤 博志, 古川翔太, 大津由紀, 西谷陽子
    • Organizer
      2021年度アルコール・薬物依存関連学会合同学術総会(第56回日本アルコール・アディクション医学会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] アルコール・薬物の検出の実態と検出方法:オーバービュー2019

    • Author(s)
      西谷陽子
    • Organizer
      2019年度日本アルコール・アディクション医学会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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