| Project/Area Number |
18K10144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 58050:Fundamental of nursing-related
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2021: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 皮膚 / 洗浄剤 / 線維芽細胞 / 創傷治癒 / 表皮3次元培養モデル / 創傷 / 皮膚3次元培養 / BMP |
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| Outline of Final Research Achievements |
The aim of this study was to determine whether there are differences between skin cleansers on wound healing and, if so, which ones do not interfere with wound healing. We investigated the effects of cleansing agents on a three-dimensional culture model of full-thickness skin and a single-layer dermal fibroblast culture.
When the 3D culture model was scratched and treated with detergent for 5 minutes per day for 5 days, the entire layer was dissolved and it was impossible to make sections. A monolayer of fibroblasts was exposed to a single wash of detergent. In the group treated with a skin clenser for general, the number of cells and the amount of collagen increased in a dose-dependent manner. In the group treated with the cleanser for sensitive skin, the number of cells increased compared to before treatment with the cleanser, but the amount of intracellular collagen was lower than that of the general cleanser.
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| Academic Significance and Societal Importance of the Research Achievements |
創傷治癒に寄与する皮膚線維芽細胞の増殖やコラーゲン量について検討した。今回検討した洗浄剤では、細胞数と細胞内コラーゲン量に対する影響は異なる傾向があった。創傷治癒に対する洗浄剤の種類による違いについては、明確に示すことができなかった。今後、細胞数は多いがコラーゲン量が少ないことに対するより詳細な検討や、動物実験などでの生体における検討によって明確にしていく必要があると考えられる。
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