| Project/Area Number |
18K10782
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 59010:Rehabilitation science-related
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| Research Institution | Kagoshima University |
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Principal Investigator |
Yoshida Akira 鹿児島大学, 医歯学域鹿児島大学病院, 講師 (40347109)
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| Co-Investigator(Kenkyū-buntansha) |
大渡 昭彦 鹿児島大学, 医歯学域医学系, 准教授 (30295282)
下堂薗 恵 鹿児島大学, 医歯学域医学系, 教授 (30325782)
原田 雄大 鹿児島大学, 医歯学総合研究科, 客員研究員 (30755228)
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| Project Period (FY) |
2018-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | マイクロダイアリシス / 神経因性膀胱 / 脳梗塞 / ラット |
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| Outline of Final Research Achievements |
Using the photosensitizing dye rose bengal, we compared urination activities in stroke-induced rats and normal rats with a small animal urinary function testing device under free-moving conditions. Although our aim was to establish an overactive bladder model post-stroke, replicating this pathology under free-moving conditions was challenging. However, we developed a new experimental system that simultaneously evaluated urination activity and serotonin and dopamine dynamics in the periaqueductal gray matter using the microdialysis method. This system revealed rhythmic serotonin changes synchronized with urination activity.
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| Academic Significance and Societal Importance of the Research Achievements |
小動物用排尿機能検査装置を用いた自由行動下での排尿活動と傍中脳水道灰白質でのセロトニンとドーパミンの動態をマイクロダイリシス法で同時に評価できる実験系を確立することができた。この実験系を用いることで、これまで行われてこなかった生理的条件下での長時間の排尿活動と脳内の神経伝達物質の動態の関連の検討が可能となり、排尿に関わる脳内の神経機構の解明や神経因性膀胱の病態の解明、新たな治療薬の開発につながることが期待される。
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