Elucidation of a cell and the molecular mechanism in the tendon-ligament insertion department formation by the dynamics stimulation

• Project/Area Number: 18K10846
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59020:Sports sciences-related
• Research Institution: Ibaraki University
• Principal Investigator: Nakajima Hiromi 茨城大学, 農学部, 特命研究員 (30323215)
• Co-Investigator(Kenkyū-buntansha): 六崎 裕高 茨城県立医療大学, 保健医療学部, 教授 (50550927)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 前十字靱帯 / 膝蓋腱 / アキレス腱 / 付着部軟骨層 / 軟骨細胞 / 細胞外基質タンパク / ペリオスチン / 前十字靭帯 / Sox9 / Ⅰ型コラーゲン / Ⅱ型コラーゲン / 腱・靱帯付着部 / 軟骨層形成 / 力学刺激 / 軟骨細胞活性 / グリコサミノグリカン / タイドマーク / 腱・靭帯付着部 / 形成過程 / 細胞・分子メカニズム

Outline of Final Research Achievements

The developmental processes, Sox9 and type 2 collagen-expression of fibrocartilage layers during anterior cruciate ligament (ACL), quadriceps tendon (QT), patellar tendon (PT) and Achilles tendon (AT) insertions differed substantially in rabbits. These differences have a possibility that the difference among mechanical environments, such as muscular strength and load, has influenced. Periostin influenced the development of the fibrocartilage layer extracellular matrix of ACL insertion in mice. Our results contribute to the administration of appropriate treatments based on the age, and aid in the development of methods for regenerating enthesis fibrocartilage layers.

Academic Significance and Societal Importance of the Research Achievements

ウサギの成長過程における腱・靱帯付着部軟骨層の発達を軟骨細胞と細胞外基質タンパクに注目して検索した結果、付着部軟骨層の発達は筋肉や負荷などの機械的環境が影響していた。また、ペリオスチンノックアウトマウスを用いた成長過程の検索では、ペリオスチンはマウス前十字靱帯付着部線維軟骨層の細胞外基質の発達に影響を及ぼした。これらの結果はヒトの腱や靱帯の疾患に際して年齢に基づいて適切な治療、その後のリハビリやスポーツ復帰までの管理に貢献できる。加えて腱・靱帯付着部線維軟骨層を再生するための方法の開発に役立つ。

Research Products

• [Journal Article] Infuluence of periostin in development of fibrocartilage layers of anterior cruciate ligament insertion (2022)
  • Author(s): Hirotaka Mutsuzaki, kazuki Kuwabara, and Hiromi Nakajima,
  • Journal Title: Orthopaedics & Traumatology: Surgery & Research Volume: ー Issue: 6 Pages: 103215-103215
  • DOI: 10.1016/j.otsr.2022.103215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of fibrocartilage layers in Achilles tendon enthesis in rabbits (2021)
  • Author(s): Hirotaka Mutsuzaki, Hiromi Nakajima
  • Journal Title: Journal of Rural Medicine Volume: 16 Issue: 3 Pages: 160-164
  • DOI: 10.2185/jrm.2021-015
  • NAID: 130008060643
  • ISSN: 1880-487X, 1880-4888
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Differences in the Development of Fibrocartilage Layers in the Quadriceps Tendon and Patellar Tendon Insertions in Rabbits (2020)
  • Author(s): Hirotaka Mutsuzaki, and Hiromi Nakajima
  • Journal Title: Orthopaedic Journal of Sports Medicine Volume: 8 Issue: 12 Pages: 1-8
  • DOI: 10.1177/2325967120966418
  • Peer Reviewed / Open Access
• [Journal Article] Development of fibrocartilage layers in the anterior cruciate ligament insertion in rabbits (2019)
  • Author(s): Hirotaka Mutsuzaki, Hiromi Nakajima, Maika Someji and Masataka Sakane
  • Journal Title: BMC Musculoskeletal Disorders Volume: 20 Issue: 1 Pages: 1-10
  • DOI: 10.1186/s12891-019-2614-0
• [Presentation] 前十字靭帯(ACL)付着部軟骨層の形成過程におけるペリオスチンの関与 (2021)
  • Author(s): 桒原 一輝、中島 弘美、六崎 裕高
  • Organizer: 日本畜産学会 第128回大会 形態・生理
• [Presentation] ウサギ出生後の膝蓋靭帯及び大腿四頭筋腱付着部軟骨層の形成過程 (2018)
  • Author(s): 髙木璃子、六崎裕高、中島弘美
  • Organizer: 第161回日本獣医学会学術集会・獣医解剖分科会