Prediction factors of life-style related disease strongly associated with change of adiposity - analysis of exsosome -

• Project/Area Number: 18K11020
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Osaka University
• Principal Investigator: Nishida Makoto 大阪大学, キャンパスライフ健康支援センター, 特任准教授 (00379273)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生活習慣病 / adiposity / エクソソーム / 血清マーカー / リスク因子 / 肥満 / 脂肪 / 健康診断

Outline of Final Research Achievements

Aim of this study is identification of new predictive markers for life style-related disease, by using the classification according to long-term changes of the adiposity and the risks of life style-related disease. We have been studying an aging-related factor, αklotho, and newly identified the association of αklotho with future hyperglycemia and with response to treatment of cardiac failure. However, αklotho did not show the long-term association with the changes of adiposity and risks of life style-related disease. Next, comprehensive analysis of exosome derived miRNA was performed in 10 years-cryopreserved serum, but it failed to demonstrate significant markers for long-term changes of risks. Further studies need to identify the new long-term markers for lifestyle related disease.

Academic Significance and Societal Importance of the Research Achievements

生活習慣病リスクは中高年に増加する。リスクの増加した人には保健指導を行うが、生活習慣が固定してしまった中高年では長続きする人は少なく結局薬物治療へと移行する。生活習慣が固定していない若年時に将来の生活習慣病リスクが予測できるマーカーがあれば、介入ターゲットを絞って指導し、その介入効果をモニターすることも可能となる可能性がある。そこで本研究では、新たな方法でそのマーカーを探索し、今後の方向性を示した。

Research Products

• [Journal Article] Serum Klotho Levels Contribute to the Prevention of Disease Progression. (2021)
  • Author(s): Nakanishi K, Nishida M, Taneike M, Yamamoto R, Moriyama T, Yamauchi-Takihara K.
  • Journal Title: Int J Gen Med. Volume: 14 Pages: 229-236
  • DOI: 10.2147/ijgm.s291437
  • Peer Reviewed / Open Access
• [Journal Article] Alpha-Klotho is a novel predictor of treatment responsiveness in patients with heart failure. (2021)
  • Author(s): Taneike M, Nishida M, Nakanishi K, Sera F, Kioka H, Yamamoto R, Ohtani T, Hikoso S, Moriyama T, Sakata Y, Yamauchi-Takihara K.
  • Journal Title: Sci Rep. Volume: 11 Issue: 1 Pages: 2058-2058
  • DOI: 10.1038/s41598-021-81517-9
  • Peer Reviewed / Open Access
• [Journal Article] Body mass index modifies the association between frequency of alcohol consumption and incidence of hypertension in men but not in women: a retrospective cohort study. (2020)
  • Author(s): Nishigaki D, Yamamoto R, Shinzawa M, Kimura Y, Fujii Y, Aoki K, Tomi R, Ozaki S, Yoshimura R, Taneike M, Nakanishi K, Nishida M, Yamauchi-Takihara K, Isaka Y, Moriyama T.
  • Journal Title: Hypertens Res. Volume: 43 Issue: 4 Pages: 322-330
  • DOI: 10.1038/s41440-019-0382-8
  • Peer Reviewed
• [Journal Article] Rivaroxaban Suppresses the Progression of Ischemic Cardiomyopathy in a Murine Model of Diet-Induced Myocardial Infarction (2019)
  • Author(s): Liu J, Nishida M, Inui H, Chang J, Zhu Y, Kanno K, Matsuda H, Sairyo M, Okada T, Nakaoka H, Ohama T, Masuda D, Koseki M, Yamashita S, Sakata Y.
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 26 Issue: 10 Pages: 915-930
  • DOI: 10.5551/jat.48405
  • NAID: 130007722198
  • ISSN: 1340-3478, 1880-3873
  • Year and Date: 2019-10-01
  • Peer Reviewed / Open Access
• [Journal Article] An Implication of alpha-Klotho as the Predictive Factor of Stress: A Cross-sectional Study (2019)
  • Author(s): Kaori Nakanishi, Makoto Nishida, Manabu Taneike, Ryohei Yamamoto, Hiroyoshi Adachi, Toshiki Moriyama, Keiko Yamauchi-Takihara
  • Journal Title: Journal of Investigative Medicine Volume: 印刷中 Issue: 7 Pages: 1082-1086
  • DOI: 10.1136/jim-2018-000977
  • Peer Reviewed
• [Journal Article] Shotgun proteomic analysis reveals proteome alterations in HDL of patients with cholesteryl ester transfer protein deficiency. (2019)
  • Author(s): Okada T, Ohama T, Takafuji K, Kanno K, Matsuda H, Sairyo M, Zhu Y, Saga A, Kobayashi T, Masuda D, Koseki M, Nishida M, Sakata Y, Yamashita S.
  • Journal Title: J Clin Lipidol Volume: 印刷中 Issue: 2 Pages: 317-325
  • DOI: 10.1016/j.jacl.2019.01.002
  • Peer Reviewed / Open Access
• [Journal Article] An implication of Klotho-related molecules in different smoking-related health outcomes between men and women (2018)
  • Author(s): Nakanishi Kaori、Nishida Makoto、Yamamoto Ryohei、Koseki Masahiro、Moriyama Toshiki、Yamauchi-Takihara Keiko
  • Journal Title: Clin Chim Acta Volume: 476 Pages: 44-48
  • DOI: 10.1016/j.cca.2017.11.007
  • Peer Reviewed / Open Access
• [Presentation] The Role of Th1 and Th17 during Myocardial Infarction and Inhibitory Effect of Probucol on Th1 in SR-BI KO/ApoeR61h/h Mice (2020)
  • Author(s): Inui Hiroyasu, Nishida Makoto, Koseki Masahiro, Kanno Kotaro, Okada Takeshi, Ohhama Toru, Yamashita Shizuya, Sakata Yasushi
  • Organizer: 第84回日本循環器学会学術集会
• [Presentation] 高コレステロール食誘発心筋梗塞モデルマウスにおけるT細胞の関与の検討 (2019)
  • Author(s): 乾 洋勉, 劉 静一, 西田 誠, 常 久洋, 朱 頴広, 冠野 昂太郎, 松田 響, 岡田 健志, 大濱 透, 小関 正博, 山下 静也
  • Organizer: 第51回日本動脈硬化学会総会
• [Presentation] 10年間の腹囲・BMIの変動と危険因子、生活習慣の関連 (2018)
  • Author(s): 西田 誠, 中西 香織, 小関 正博, 種池 学, 山本 陵平, 山下 静也, 守山 敏樹, 瀧原 圭子
  • Organizer: 第50回日本動脈硬化学会総会
• [Presentation] 性別とBMIによって、飲酒による高血圧発症リスクが異なる (2018)
  • Author(s): 鶴飼 朱美, 山本 陵平, 明石 美智子, 梅野 有希, 川村 淳子, 熊谷 一代, 村井 教子, 姫ヶ迫 由美, 中西 香織, 種池 学, 西田 誠, 瀧原 圭子, 守山 敏樹
  • Organizer: 第56回全国大学保健管理研究集会