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Elucidation of the biological defense mechanism of chondroitin sulfate on the intestinal tract

Research Project

Project/Area Number 18K11068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 59040:Nutrition science and health science-related
Research InstitutionHirosaki University

Principal Investigator

Nakamura Toshiya  弘前大学, 保健学研究科, 教授 (00155847)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsコンドロイチン硫酸 / プロテオグリカン / 経口投与 / 腸管上皮 / IL6 / 機能性食品 / 消化産物 / 消化管上皮細胞 / 生体防御
Outline of Final Research Achievements

We purified PG from a proteoglycan (PG) sample derived from salmon nasal cartilage and administered it orally to mice to investigate the structure of PG digestion products in the small intestinal contents. The results showed that the core protein of PG was degraded, but the chain length and sulfate groups of chondroitin sulfate (ChS) glycans were not changed. Next, we established small and large intestine models of human colon carcinoma-derived Caco-2 cells in a culture system, and examined the cellular response to commercial ChS in these models. The results showed that ChS suppressed the gene expression of interleukin-6 induced by tumor necrosis factor-α. This suggests that oral intake of PG may have an anti-inflammatory effect on the intestinal tract.

Academic Significance and Societal Importance of the Research Achievements

プロテオグリカンやコンドロイチン硫酸による腸管上皮細胞への直接影響について生体防御の観点からの研究はこれまでに報告がなく,同類の多糖鎖の機能としてはヒアルロン酸に続く新たな機能の発見となる。このプロテオグリカンの経口摂取による腸管の抗炎症効果の可能性が示唆されたことより,プロテオグリカンおよびコンドロイチン硫酸の食品への応用のしやすさ(水に溶けやすい,中性条件で熱に強い,無味無臭である,等)も相まって,幼児から高齢者に至る国民の健康維持への貢献が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 6 results) Presentation (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Radiation Safety and Public Health for Radiological Professionals: Meeting Report on The 5th Educational Symposium on Radiation and Health (ESRAH) by Young Scientists in 2018.2021

    • Author(s)
      Yamashina Hiroko, Terashima Marin, Oikawa Joma, Naijo Shingo, Miyao Tamao, Yachi Yoshie, Matsuya Yusuke, Yamaguchi Masaru, Tsujiguchi Takakiyo, Saga Ryo, Jun Hu, Goh Valerie Swee Ting, Nakamura Toshiya, Hosokawa Yoichiro, Date Hiroyuki.
    • Journal Title

      Radiation Environment and Medicine

      Volume: 10 Pages: 48-54

    • NAID

      40022494206

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of Thr328 in hyaluronan synthase 2 is essential for hyaluronan synthesis2020

    • Author(s)
      Kasai Kosuke、Kuroda Yoshiyuki、Takabuchi Yutaro、Nitta Akihide、Kobayashi Takashi、Nozaka Hiroyuki、Miura Tomisato、Nakamura Toshiya
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 533 Issue: 4 Pages: 732-738

    • DOI

      10.1016/j.bbrc.2020.08.093

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metal coordination by L-amino acid oxidase derived from flounder Platichthys stellatus is structurally essential and regulates antibacterial activity2020

    • Author(s)
      Kasai Kosuke、Ito Yudai、Nitta Akihide、Ariyoshi Kentaro、Nakamura Toshiya、Miura Tomisato
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 104 Issue: 22 Pages: 9645-9654

    • DOI

      10.1007/s00253-020-10914-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dermatan sulfate oligosaccharides having reducing end 2, 5-anhydro-D-talose inhibit bovine testicular hyaluronidase activity2019

    • Author(s)
      Kakizaki I, Koizumi H, Kobayashi T, Nakamura T, Majima M.
    • Journal Title

      Carbohydrate Research

      Volume: 483 Pages: 107754-107754

    • DOI

      10.1016/j.carres.2019.107754

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lessons From a Japan-Korea Collaboration on Medical Response Training for a Nuclear or Radiological Emergency, Assuming Mass Casualty2019

    • Author(s)
      Tsujiguchi T, Kitajima M, Tomisawa T, Shiroto Y, Yamada R, Saito K, Hanada H, Jin Y-W, Cho M, Jang S, Kim H, Pak M, Nakamura T.
    • Journal Title

      Disaster Medicine and Public Health Preparedness

      Volume: Sep 30 Issue: 4 Pages: 1-2

    • DOI

      10.1017/dmp.2019.88

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells2019

    • Author(s)
      Saga R, Hasegawa K, Murata K, Chiba M, Nakamura T, Okumura K, Tsuruga E, Hosokawa Y.
    • Journal Title

      Oncology Letters

      Volume: 17 Pages: 3555-3561

    • DOI

      10.3892/ol.2019.9990

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteoglycan-substrate gel zymography for the detection of chondroitin sulfate-degrading enzymes2018

    • Author(s)
      Kobayashi T, Kakizaki I, Nakamura T
    • Journal Title

      Analytical Biochemistry

      Volume: 568 Pages: 51-52

    • DOI

      10.1016/j.ab.2018.12.010

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blackcurrant Anthocyanins Increase the Levels of Collagen, Elastin, and Hyaluronic Acid in Human Skin Fibroblasts and Ovariectomized Rats2018

    • Author(s)
      Nanashima Naoki、Horie Kayo、Maeda Hayato、Tomisawa Toshiko、Kitajima Maiko、Nakamura Toshiya
    • Journal Title

      Nutrients

      Volume: 10 Issue: 4 Pages: 495-495

    • DOI

      10.3390/nu10040495

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meeting report on "The 4th Educational Symposium on Radiation and Health (ESRAH) by Young Scientists in 2017"2018

    • Author(s)
      Saga R, Tsujiguchi T, Yamaguchi M, Fukushi Y, Fujishima Y, Matsuya Y, Oikawa J, Terashima M, Date H, Nakamura T, Hosokawa Y.
    • Journal Title

      Radiation Environment and Medicine

      Volume: 7 Pages: 121-124

    • NAID

      40021646787

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マウスに経口投与されたプロテオグリカンの小腸における消化産物2020

    • Author(s)
      工藤 海,小林 孝,葛西宏介,横山洸大,作田泰宏,中村敏也
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] サケ鼻軟骨プロテオグリカンを使ったコンドロイチン硫酸分解酵素の検出2018

    • Author(s)
      小林 孝、柿崎育子、中村敏也
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] グリコサミノグリカン分解酵素又はその阻害物質の検出方法2018

    • Inventor(s)
      中村敏也,柿崎育子,小林 孝
    • Industrial Property Rights Holder
      弘前大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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