| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
In this study, analyses of motility and DNA integrity of sperm collected from male mice after γ-ray irradiation were performed. In addition, the effect of inflammation during spermatogenesis on the viability of germ cells and sperm characteristics was investigated. Irradiation with γ-ray to the neonatal mice (2-14 days of age) affected DNA integrity of sperm, whereas there was no effect on the sperm motility. When the oocytes were fertilized in vitro with spermatozoa collected from male mice that were induced continuous inflammation by LPS administration, the rate of fertilization was lower than that of IVF eggs in the control group. Embryonic development of IVF eggs using LPS-administrated male was also impaired after the 4-cell stage. These results demonstrated that spermatozoa derived from male mice irradiated with γ-ray at the neonatal stage accumulated DNA damages. There was a possibility that continuous inflammation in testis decreased the fertilizability of spermatozoa.
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