| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2021: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we aimed to establish a system that enables irradiation of α-rays to a single targeted cell and to detect tissue/organ specificity of bystander effects around irradiated cells using this system. As a result, it was possible to irradiate several units of Po-210-derived α-rays targeting the nucleus of a single human cultured cell and observe the subsequent intracellular molecular behavior in real time. We also showed that it is possible to irradiate not only smooth cultured cells but also cell masses in three-dimensional culture. Although the bystander effect could not be detected clearly, we succeeded in capturing moving images of DNA double-strand break (DSB) repair-related proteins accumulating at localized sites in the nucleus hit by α-rays.
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