Novel acitivation mechanizm of ATR-ATRIP kinase upon DNA replication stress
| Project/Area Number |
18K11642
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 63020:Radiation influence-related
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Ishiai Masamichi 国立研究開発法人国立がん研究センター, 研究所, 施設長 (90298844)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DNA損傷応答 / DNA修復 / フォーカス形成 / ATRIP / ATR |
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| Outline of Final Research Achievements |
The DNA damage response is critical for cells to prevent from genotoxic stress such as DNA damage. ATR kinase is one of the central players in this system. ATR makes a complex with ATRIP, activated under DNA replication stress by two independent mechanisms. To identify novel ATR-ATRIP activation pathway, we undertook siRNA screening against mitomycin C and hydroxy urea treated ATRIP-GFP expressing cells by evaluating both ATRIP and RPA2 foci formation efficiency. We obtained 29 candidate genes. We further evaluate these candidates.
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| Academic Significance and Societal Importance of the Research Achievements |
DNA損傷応答は細胞の生存に必須であり、その破綻はがん等の疾患の原因となることが知られている。本研究は、その中でも特に重要なATRに注目し、既存の2つの活性化経路に加えて未知の活性化経路が存在する可能性を検討するものである。従って、本研究課題は、発がん等の疾患の基礎研究としての側面や、これらを標的とした新たな治療薬・新弾薬の開発につながる基礎的知見を提供する可能性がある。
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Report
(6 results)
Research Products
(19 results)
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[Journal Article] Bacterial SOS Genes mucAB/umuDC Promote Mouse Tumors by Activating Oncogenes Nedd9/Aurkb via a miR-145 Sponge2020
Author(s)
Tanooka H, Inoue A, Takahashi RU, Tatsumi K, Fujikawa K, Nagao T, Ishiai M, Chiwaki F, Aoyagi K, Sasaki H, Ochiya T.
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Journal Title
Mol Cancer Res.
Volume: 18
Issue: 9
Pages: 1271-1277
DOI
Related Report
Peer Reviewed
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[Journal Article] USP42 enhances homologous recombination repair by promoting R-loop resolution with a DNA-RNA helicase DHX92020
Author(s)
Matsui M, Sakasai R, Abe M, Kimura Y, Kajita S, Torii W, Katsuki Y, Ishiai M, Iwabuchi K, Takata M, Nishi R.
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Journal Title
Oncogenesis
Volume: 9
Issue: 6
Pages: 60-60
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Bacterial SOS genes mucAB/umuDC promote mouse tumors by activating oncogenes Nedd9/Aurkb via a miR-145 sponge2020
Author(s)
Tanooka H, Inoue A, Takahashi R, Tatsumi K, Fujikawa K, NagaoT, Ishiai M, Chiwaki F, Aoyagi K, Sasaki H, Ochiya T.
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Journal Title
Molecular Cancer research
Volume: -
Related Report
Peer Reviewed
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