• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the environmental response mechanism of plants as an ozone adaptation strategy

Research Project

Project/Area Number 18K11673
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 63040:Environmental impact assessment-related
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

AONO MITSUKO  国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, 副研究センター長 (10202491)

Co-Investigator(Kenkyū-buntansha) 佐治 光  国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, シニア研究員 (00178683)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsオゾン / シロイヌナズナ / 植物環境応答 / 環境適応戦略 / アポプラスト / ブナ
Outline of Final Research Achievements

We isolated an ozone-tolerant plant from the Full-length cDNA overexpressor lines of Arabidopsis thaliana. This line contains a cDNA encoding a protein X that is a member of the phytocyanin group. We confirmed that X is responsible for the ozone tolerance by separately generating transgenic plants that overexpress this cDNA. The stomatal conductance of the X-overexpressing plant was similar to that of the wild-type plant in the absence of ozone, but the ozone-induced reduction in stomatal conductance observable in the wild-type plant was not observed in the X-overexpressing plant. Therefore, both of the two ozone-induced responses, stomatal closure and foliar cell death, can be suppressed in the X-overexpressing plant. Generations and analyses of transgenic plants that produce the fusion protein of X and a green-fluorescent protein indicate that X exists at the apoplastic region of plant cells where X may regulate cell-wall metabolism or a signaling involved in the stress response.

Academic Significance and Societal Importance of the Research Achievements

オゾンは光化学オキシダントの主成分であり、森林衰退や農作物被害の原因となっている。気候変動による気温の上昇等に伴い、今後地球規模で対流圏のオゾン濃度が上昇すると考えられ、特に大都市近郊における影響拡大が懸念されている。一方、植物は過酷な環境にも適応して生き延びるため環境応答機構を進化させてきた。これまでの研究により、植物がオゾンに適応するための環境応答機構(オゾン耐性機構)がある程度明らかになってきた。本研究課題では、シロイヌナズナ突然変異系統を用いてオゾン耐性機構に関与する新たな遺伝子を見出し、植物の高オゾン濃度への適応戦略として実際に機能している機構の一端を明らかにすることを目指した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2021 2020 2019

All Presentation (5 results)

  • [Presentation] Enhancement of ozone tolerance by overexpression of a gene encoding a phytocyanin in Arabidopsis.2021

    • Author(s)
      Saji S., Saji H., Sage-Ono K., Ono M., Nakajima N., Inoue T., Aono M.
    • Organizer
      The 62nd Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物にオゾン耐性を付与する新規遺伝子の解析(2)2020

    • Author(s)
      佐治章子, 佐治光, 小野公代, 小野道之, 中嶋信美, 井上智美, 青野光子
    • Organizer
      第61回大気環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Analyses Of A Novel Gene That Enhances Ozone Tolerance Of Plants.2020

    • Author(s)
      Saji S., Saji H., Sage-Ono K., Ono M., Nakajima N., Inoue T., Aono M.
    • Organizer
      The 61th Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2020 Annual Research Report
  • [Presentation] Analyses of A Novel Gene That Enhances Ozone Tolerance of Plants2020

    • Author(s)
      Saji S., Saji H., Sage-Ono K., Ono M., Nakajima N., Inoue T., Aono M.
    • Organizer
      The 61th Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2019 Research-status Report
  • [Presentation] 植物にオゾン耐性を付与する新規遺伝子の解析2019

    • Author(s)
      佐治章子, 佐治光, 小野公代, 小野道之, 中嶋信美, 井上智美, 青野光子
    • Organizer
      第60回大気環境学会年会
    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi