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A new environmental DNA analysis to overcome inhibition: construction of a Biomass estimation equation with inhibition factors

Research Project

Project/Area Number 18K11678
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 64010:Environmental load and risk assessment-related
Research InstitutionNara Women's University

Principal Investigator

Katano Izumi  奈良女子大学, 自然科学系, 准教授 (90414995)

Co-Investigator(Kenkyū-buntansha) 杉山 裕子  岡山理科大学, 理学部, 准教授 (40305694)
土居 秀幸  兵庫県立大学, シミュレーション学研究科, 准教授 (80608505)
高原 輝彦  島根大学, 学術研究院農生命科学系, 准教授 (10536048)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords環境DNA / 泥 / 細菌 / 植物プランクトン / 有機物 / POM / PIM / TP / TN / DOC / クロロフィル / 細菌数 / ゼブラフィッシュ / 生物多様性保全 / 水質
Outline of Final Research Achievements

The ultimate goal of this study is to develop a biomass estimation equation using environmental DNA (eDNA) detection with environmental inhibition. We have conducted experiments with gradients in the quantity and quality of fine particles in water to determine the amount of eDNA inhibition. As results, we have found that the amount of eDNA inhibition increases with the amount of organic matter in suspended soid in environmental water. From manipulation experiments focusing on amount of bacteria and water flow showed that the amount of eDNA released from zebrafish and the eDNA decomposition were strongly affected first by the water flow and then by the amount of bacteria.

Academic Significance and Societal Importance of the Research Achievements

現在,環境DNA(eDNA)技術は,非侵襲的な生物調査方法として,外来種対策や希少種対策など行政による環境政策への導入も進んできている。しかし,野外環境では,その場に生息する対象生物の生物量と,eDNAの有無・量が相関しないことがよく認知されてきている。これは,環境中の阻害要因がeDNAの検出を阻害するためと言われている。eDNA技術による生物調査をより確実にするためには,野外における様々なe DNA検出阻害を加味した生物量推定式の作成が必要である。本研究は,野外調査や室内実験を行なうことにより,この式を得ることを最終目標としている。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (38 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Open Access: 11 results) Presentation (22 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Montana University/Cornell University(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Reconciling biodiversity conservation and flood risk reduction: the new strategy for freshwater protected areas.2022

    • Author(s)
      Akasaka T, Mori T, Ishiyama N, Takekawa Y, Kawamoto T, Inoue M, Mitsuhashi H, Kawaguchi Y, Ichiyanagi H, Onikura N, Miyake Y, Katano I, Akasaka M, Nakamura F.
    • Journal Title

      Diversity and distributions

      Volume: - Issue: 6 Pages: 1191-1201

    • DOI

      10.1111/ddi.13517

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 同所に生息する濾過食者シマトビケラ属2種の体内脂質割合2022

    • Author(s)
      輪地紗良,片野泉
    • Journal Title

      陸水研究

      Volume: in press

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of sediment replenishment on riverbed environments and macroinvertebrate assemblages downstream of a dam.2021

    • Author(s)
      Katano I, Negishi JN, Minagawa T, Doi H, Kawaguchi Y, Kayaba Y.
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1

    • DOI

      10.1038/s41598-021-86278-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A unified framework for herbivore-to-producer biomass ratio reveals the relative influence of four ecological factors.2021

    • Author(s)
      Kazama T, Urabe J, Yamamichi M, Tokita K, Xuwang Y, Katano I, Doi H, Yoshida T, Hairston Jr. NG.
    • Journal Title

      Communucation Biology

      Volume: 4 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s42003-020-01587-9

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exploring a legendary giant squid: An environmental DNA approach.2020

    • Author(s)
      Wada T, Doi H, Togaki D, Kaida R, Nagano M, Katano I, Suzuki M, Ohtani T, Mitsuhashi H.
    • Journal Title

      Marine Biology

      Volume: 167 Issue: 11 Pages: 1-7

    • DOI

      10.1007/s00227-020-03773-z

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of sampling season and location in fish environmental DNA metabarcording in dam reservoirs.2020

    • Author(s)
      Hayami K, Sakata MK, Inagawa T, Okitsu J, Katano I, Nakai K, Ichiyanagi H, Gotoh RO, Miya M, Sato H, Yamanaka H, Minamoto T.
    • Journal Title

      Ecology and Evolutions

      Volume: 10 Issue: 12 Pages: 5354-5367

    • DOI

      10.1002/ece3.6279

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of freshwater mussels (Sinanodonta spp.) in artificial ponds through environmental DNA: a comparison with traditional hand collection methods.2020

    • Author(s)
      Togaki D, Doi H, and Katano I.
    • Journal Title

      Limnology

      Volume: 21 Issue: 1 Pages: 59-61

    • DOI

      10.1007/s10201-019-00584-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ダム上下流・土砂還元上下流における濾過食者・刈取食者の体内脂質割合と餌資源の化学量バランス.2020

    • Author(s)
      佐藤智春,土居秀幸,片野泉
    • Journal Title

      陸水研究

      Volume: 7 Pages: 19-27

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 要注意外来種ミシシッピアカミミガメのため池群における分布決定要因:水質および人間による捨てやすさを考慮して.2020

    • Author(s)
      覺田青空,土居秀幸,片野泉
    • Journal Title

      陸水研究

      Volume: 7 Pages: 9-18

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of stream grazers with different functional traits on the spatial heterogeneity of periphyton mats.2019

    • Author(s)
      Katano I, Doi H.
    • Journal Title

      PeerJ

      Volume: 7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatial structures of fungal DNA assemblages revealed with eDNA metabarcoding in a forest river network in western Japan.2019

    • Author(s)
      Matsuoka S, Sugiyama Y, Sato H, Katano I, Harada K, Doi H.
    • Journal Title

      Metabarcoding and Metagenomics

      Volume: 3

    • DOI

      10.3897/mbmg.3.36335

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA.2019

    • Author(s)
      Uchii K, Doi H, Okahashi T, Katano I, Yamanaka H, Sakata KM, Minamoto T.
    • Journal Title

      Environmental DNA

      Volume: 1 Issue: 4 Pages: 359-367

    • DOI

      10.1002/edn3.37

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental DNA detection and quantification of invasive red-eared sliders, Trachemy scripta elegans, in ponds and the influence of water quality.2019

    • Author(s)
      Kakuda A, Doi H, Souma R, Nagano M, Minamoto T, Katano I.
    • Journal Title

      PeerJ 7

      Volume: 7 Pages: e8155-e8155

    • DOI

      10.7717/peerj.8155

    • NAID

      120006824001

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ダム上・下流における造網性トビケラ2種の体内脂質割合.2019

    • Author(s)
      小林優太郎,角絢香,土居秀幸,片野泉.
    • Journal Title

      陸水研究

      Volume: 6 Pages: 11-19

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] A shady phytoplankton paradox: when phytoplankton increases under low light.2018

    • Author(s)
      Yamamichi M, Kazama T, Tokita K, Katano I, Doi H, Yoshida T, Hairston Jr NG, Urabe J.
    • Journal Title

      Proc. R. Soc. B

      Volume: 285 Issue: 1882 Pages: 20181067-20181067

    • DOI

      10.1098/rspb.2018.1067

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 半水生哺乳類ニホンカワネズミの生息地推定に対する環境DNA法の検討2021

    • Author(s)
      塩塚菜生,中村匡聡,土居秀幸,片野泉.
    • Organizer
      応用生態工学第24回研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダム下流における支川流入・土砂還元が河床環境と底生動物群集に与える影響2021

    • Author(s)
      原直子,片野泉,土居秀幸,根岸淳二郎,皆川朋子,萱場祐一
    • Organizer
      応用生態工学第24回研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダムによる餌環境変化が濾過食者シマトビケラ科の栄養状態に及ぼす影響2021

    • Author(s)
      輪地紗良,土居秀幸,田代喬,原田守啓,竹門康弘,片野泉
    • Organizer
      応用生態工学第24回研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 環境DNAによる半水生哺乳類カワネズミChimarrogale platycephalus の活動時間の解明2021

    • Author(s)
      塩塚菜生,中村匡聡,土居秀幸,片野泉
    • Organizer
      日本陸水学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダム下流における支川流入・土砂還元が河床環境と底生動物群集に与える影響2021

    • Author(s)
      原直子,皆川朋子,根岸淳二郎,土居秀幸,萱場祐一,片野泉
    • Organizer
      日本陸水学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダムによる餌環境変化が濾過食者シマトビケラ科の栄養状態に及ぼす影響2021

    • Author(s)
      輪地紗良,土居秀幸,竹門康弘,片野泉
    • Organizer
      日本陸水学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 水中の環境 DNA 減衰過程におよぼす泥と流れの影響2021

    • Author(s)
      辻恵実,片野泉,土居秀幸
    • Organizer
      日本陸水学会近畿支部回第33回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダムによる餌環境変化と濾過食者シマトビケラ科の栄養状態2021

    • Author(s)
      輪地紗良,土居秀幸,竹門康弘,片野泉
    • Organizer
      日本陸水学会近畿支部回第33回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 木津川中流域における伝統的河川工法・聖牛により創出されたワンド・低水敷タマリの環境と生物群集2021

    • Author(s)
      中村萌,田中亜季,竹門康弘,片野泉
    • Organizer
      日本陸水学会近畿支部回第33回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 河川濾過食者の瀬内分布と濾過機能:宇治川でのシマトビケラ科幼虫による検証2021

    • Author(s)
      坂本菜々子,原直子,輪地紗良,片野泉
    • Organizer
      日本陸水学会近畿支部回第33回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Effects of sediment replenishment on riverbed environments and macroinvertebrate assemblages downstream of a dam2021

    • Author(s)
      Izumi Katano; Junjiro Negishi; Tomoko Minagawa; Hideyuki Doi; Yuichi Kayaba
    • Organizer
      Society for Freshwater Science annual meeting 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 環境DNAによる、湿地性半翅目ヒメタイコウチ(Nepa hoffmanni)の生息密度推定:保全ビオトープにおける微生息場所の季節変化2021

    • Author(s)
      信ヶ原佐保; 片野泉; 窪田敏
    • Organizer
      日本陸水学会近畿支部会第32回研究発表会
    • Related Report
      2020 Research-status Report
  • [Presentation] 源流に生息する希少両生類ハコネサンショウウオの分布調査におけるeDNA法の活用2021

    • Author(s)
      小林凛; 片野泉
    • Organizer
      日本陸水学会近畿支部会第32回研究発表会
    • Related Report
      2020 Research-status Report
  • [Presentation] ダムによる餌環境変化が濾過食者シマトビケラ科の栄養状態に及ぼす影響2021

    • Author(s)
      輪地紗良; 片野泉
    • Organizer
      日本陸水学会近畿支部会第32回研究発表会
    • Related Report
      2020 Research-status Report
  • [Presentation] ダム河川における河床表面・間隙域の生物相比較:濁りに着目して2021

    • Author(s)
      東山芽生; 片野泉; 田中亜季
    • Organizer
      日本陸水学会近畿支部会第32回研究発表会
    • Related Report
      2020 Research-status Report
  • [Presentation] カワネズミにとって陸域由来の餌生物は重要か?2021

    • Author(s)
      塩塚菜生; 片野泉; 東信行
    • Organizer
      日本陸水学会近畿支部会第32回研究発表会
    • Related Report
      2020 Research-status Report
  • [Presentation] 環境DNA技術における阻害要因の検討:有機物に注目して.2020

    • Author(s)
      伊藤英里・覚田青空・齊藤達也・杉山裕子・伊藤雅之・土居秀幸・片野泉.
    • Organizer
      日本陸水学会近畿支部会
    • Related Report
      2019 Research-status Report
  • [Presentation] 河川表流水と間隙水域との環境DNAの比較:河床間隙を利用する生物に着目して.2020

    • Author(s)
      井口真美・小池祥平・覚田青空・土居秀幸・片野泉.
    • Organizer
      日本陸水学会近畿支部会
    • Related Report
      2019 Research-status Report
  • [Presentation] アカミミガメを対象とした野外における環境 DNA 検出阻害要因の検討 :野外での環境 DNA 検出確率の向上に向けて2018

    • Author(s)
      覺田青空,東垣大祐,源利文 ,土居秀幸 ,片野泉
    • Organizer
      応用生態工学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 底生動物群集へ及ぼす貯水ダムの影響 :メタ群集およびγ多様性に着目して2018

    • Author(s)
      佐藤智春,松岡真梨奈,水守裕一,覚田青空,土居秀幸,片野 泉
    • Organizer
      応用生態工学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 水生昆虫成虫の分散におよぼすダムの影響2018

    • Author(s)
      松岡真梨奈, 水守裕一, 布野隆之, 一柳英隆, 土居秀幸, 片野泉
    • Organizer
      応用生態工学会
    • Related Report
      2018 Research-status Report
  • [Presentation] アカミミガメを対象とした野外における環境 DNA検出阻害要因の検討:野外での環境 DNA 検出確率の向上に向けて2018

    • Author(s)
      覺田青空・東垣大祐・源利文・土居秀幸・片野泉
    • Organizer
      環境DNA学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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