Visualization of metabolic changes in cultured vascular endothelial cells subjected to mechanical stress
| Project/Area Number |
18K12037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
Ito Kazushi 秋田県立大学, システム科学技術学部, 准教授 (30507116)
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| Co-Investigator(Kenkyū-buntansha) |
きゅう 建輝 秋田県立大学, システム科学技術学部, 教授 (40244511)
常盤野 哲生 秋田県立大学, 生物資源科学部, 准教授 (50312343)
境 英一 秋田県立大学, システム科学技術学部, 准教授 (70581289)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ITO / PDMS / 細胞パターニング / 培養血管内皮細胞 / MALDI‐TOF‐MS / 導電性 / ITOフィルム / MALDI-TOF-MS / 濡れ性 / 質量分析イメージング / バイオメカニクス / メカノバイオロジー |
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| Outline of Final Research Achievements |
The aim of this study was to evaluate metabolic changes in cultured vascular endothelial cell remodeling using a combined technique of inverted fluorescence microscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). For this purpose, a composite film with excellent light transmittance and conductivity was prepared, consisting of indium tin oxide (ITO) and poly (dimethyl siloxane) (PDMS). Cell patterning could be applied to the fabricated composite film surface. Furthermore, vascular endothelial cells（ECs）cultured on the composite film showed mechanical response to flow. It was also found that the composite film can be used as a sample plate for MALDI-TOF-MS. In summary, our group constructed a complex experimental system for the patterned ECs subjected to shear stress.
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| Academic Significance and Societal Importance of the Research Achievements |
構築した実験系を用いて，血流環境を模擬した力学環境下における細胞応答を詳細に評価できる可能性があり，血管疾病および血管新生の機構解明に寄与できると期待される。
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Report
(6 results)
Research Products
(5 results)
