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Visualization of metabolic changes in cultured vascular endothelial cells subjected to mechanical stress

Research Project

Project/Area Number 18K12037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionAkita Prefectural University

Principal Investigator

Ito Kazushi  秋田県立大学, システム科学技術学部, 准教授 (30507116)

Co-Investigator(Kenkyū-buntansha) きゅう 建輝  秋田県立大学, システム科学技術学部, 教授 (40244511)
常盤野 哲生  秋田県立大学, 生物資源科学部, 准教授 (50312343)
境 英一  秋田県立大学, システム科学技術学部, 准教授 (70581289)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsITO / PDMS / 細胞パターニング / 培養血管内皮細胞 / MALDI‐TOF‐MS / 導電性 / ITOフィルム / MALDI-TOF-MS / 濡れ性 / 質量分析イメージング / バイオメカニクス / メカノバイオロジー
Outline of Final Research Achievements

The aim of this study was to evaluate metabolic changes in cultured vascular endothelial cell remodeling using a combined technique of inverted fluorescence microscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). For this purpose, a composite film with excellent light transmittance and conductivity was prepared, consisting of indium tin oxide (ITO) and poly (dimethyl siloxane) (PDMS). Cell patterning could be applied to the fabricated composite film surface. Furthermore, vascular endothelial cells(ECs)cultured on the composite film showed mechanical response to flow. It was also found that the composite film can be used as a sample plate for MALDI-TOF-MS. In summary, our group constructed a complex experimental system for the patterned ECs subjected to shear stress.

Academic Significance and Societal Importance of the Research Achievements

構築した実験系を用いて,血流環境を模擬した力学環境下における細胞応答を詳細に評価できる可能性があり,血管疾病および血管新生の機構解明に寄与できると期待される。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2021 2019 2018

All Presentation (5 results)

  • [Presentation] PDMS/ITOフィルム表面の濡れ性および細胞接着性に及ぼす加工条件の影響2021

    • Author(s)
      建部一翔、 常盤野哲生、 伊藤一志
    • Organizer
      日本機械学会東北支部第57期秋季講演会
    • Related Report
      2021 Research-status Report
  • [Presentation] ITOフィルム表面における細胞パターニング技術の構築2019

    • Author(s)
      田中 考祐,邱 建輝,境 英一,伊藤 一志
    • Organizer
      日本機械学会 2019年度年次大会
    • Related Report
      2019 Research-status Report
  • [Presentation] PLA複合材料に培養した血管内皮細胞の細胞-基質間接着性に及ぼすCNF充填量の影響2019

    • Author(s)
      伊藤 一志,平岩 佑都,邱 建輝,境 英一
    • Organizer
      日本機械学会 2019年度年次大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 射出成形したリグノセルロースナノファイバー/ポリ乳酸複合材料の機械的特性2018

    • Author(s)
      平岩 佑都, 吉田 郁弥, 伊藤 一志, 邱 建輝, 境 英一
    • Organizer
      日本機械学会東北支部第54期秋季講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] グラファイトおよびCNTを充填したPLA複合材料の導電性評価2018

    • Author(s)
      金子 壮大, 野辺 理恵, 邱 建輝, 伊藤 一志, 境 英一
    • Organizer
      日本機械学会東北支部第54期秋季講演会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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