Effect of HGF on islet and its applicability

• Project/Area Number: 18K12057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Kyoto University
• Principal Investigator: Sumi Shoichiro 京都大学, ウイルス・再生医科学研究所, 准教授 (80252906)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肝細胞増殖因子(HGF) / マクロカプセル化膵島 / エバール膜 / キトサンゲル / 皮下移植 / 血管新生 / 低酸素 / 肝細胞増殖因子（HGF） / HGF（肝細胞増殖因子） / 膵島 / マクロカプセル化

Outline of Final Research Achievements

This study showed that hepatocyte growth factor (HGF) inhibits hypoxia-induced apoptosis of islet cells. In chitosan gel that slowly releases HGF, addition of HGF into the gel enabled primary subcutaneous transplantation of macro-encapsulated islets. However, this effect lasted about 5 months and this observation suggested that durability of gel material is important for the sustained transplantation effect of this kind of macro-encapsulated islets.

Academic Significance and Societal Importance of the Research Achievements

HGFが膵島細胞の低酸素によるアポトーシスを防止することは、知る限り初めての知見で、今後の膵島移植やカプセル化膵島移植の成績向上に資する可能性がある。また、マクロカプセル化膵島の一期的皮下移植に成功したことは、１型糖尿病の患者や家族、その医療者にとって大きな進歩であり、今後、この種のカプセル化膵島が研究の主体となる可能性があり、それが持つ細胞を漏らすことなく全て回収できる機能を応用して、異種移植や未分化細胞から文化誘導した細胞の移植などに応用できる可能性がある。

Research Products

• [Journal Article] Prevascularization-free Primary Subcutaneous Transplantation of Xenogeneic Islets Coencapsulated With Hepatocyte Growth Factor. (2020)
  • Author(s): Yang SY, Yang KC, Sumi S.
  • Journal Title: Transplant Direct. Volume: 6 Issue: 11 Pages: e620-e620
  • DOI: 10.1097/txd.0000000000001078
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Our Steps toward Subcutaneous Transplantation of Macro-Encapsulated Islets. (2019)
  • Author(s): Sumi S, Yang SY, Canning P, Yang KC.
  • Journal Title: OBM Transplantation Volume: 3 Issue: 5 Pages: 16-16
  • DOI: 10.1016/j.transproceed.2019.01.135
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Hepatocyte growth factor improves macro-encapsulated islet engraftment in subcutaneous transplantation. (2019)
  • Author(s): Yang SY, Yang KC, Sumi S.
  • Organizer: Asian Pancreas and Islet Transplant Association,
  • Int'l Joint Research
• [Presentation] Co-encapsulating hepatocyte growth factor enhances engraftment and function to xenogeneic islet in subcutaneous transplantation. (2019)
  • Author(s): Yang SY, Yang KC, Sumi S.
  • Organizer: International Xenotransplantation Association 15th Congress
  • Int'l Joint Research
• [Presentation] The effect of hepatocyte growth factor supplement to type 1 diabetes treatment. (2019)
  • Author(s): Yang SY, Yang KC, Sumi S.
  • Organizer: American Pancreatic Association 50th Anniversary Meeting,
  • Int'l Joint Research
• [Presentation] Hepatocyte growth factor enhance islet engraftment in subcutaneous transplantation. (2019)
  • Author(s): Yang SY, Yang KC, Sumi S.
  • Organizer: 第46回日本膵・膵島移植研究会
• [Presentation] Hepatocyte growth factor improves islet engraftment in subcutaneous transplantation. (2019)
  • Author(s): Yang SY, Yang KC, Sumi S.
  • Organizer: 第18回日本再生医療学会総会
• [Presentation] Hepatocyte growth factor improves macroencapsulated islet engraftment in subcutaneous transplantation (2019)
  • Author(s): Sin-Yu Yang, Kai-Chiang Yang, Shoichiro Sumi
  • Organizer: 1st meeting of Asia Pancreas and Islet Transplantation Association
  • Int'l Joint Research
• [Presentation] Hepatocyte growth factor enhance islet engraftment in subcutaneous transplantation (2019)
  • Author(s): 楊 心妤、楊 凱強、角 昭一郎
  • Organizer: 第46回日本膵・膵島移植研究会
• [Presentation] Hepatocyte growth factor improves islet engraftment in subcutaneous transplantation (2019)
  • Author(s): 楊 心妤、楊 凱強、角 昭一郎
  • Organizer: 第18回日本再生医療学会総会
• [Remarks] 京都大学大学院医学研究科・医学部 臓器・器官形成応用
  • URL: https://www.med.kyoto-u.ac.jp/organization-staff/research/doctoral_course/r-091/
• [Remarks] 京都大学 ウイルス・再生医科学研究所 臓器・器官形成応用分野
  • URL: https://www.infront.kyoto-u.ac.jp/research/lab16/