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Cancer diagnosis at the single cell level by microscopic Brillouin scattering spectroscopy

Research Project

Project/Area Number 18K12058
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionOsaka University

Principal Investigator

Ishitobi Hidekazu  大阪大学, 生命機能研究科, 准教授 (20372633)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsブリルアン散乱 / 1細胞レベル / 癌診断 / 粘弾性 / エタロン / 癌細胞 / 顕微鏡 / 分光
Outline of Final Research Achievements

I succeeded in improving the S/N of the Brillouin scattered light component by cutting the Rayleigh light reflected at the substrate/sample interface using annular illumination and an iris. In addition, the Brillouin scattering spectra in the cell nucleus and cytoplasm of HeLa cells were measured, and it was confirmed that there was a significant difference in the amount of Brillouin shift and the half width. Furthermore, the Brillouin image of neural cells was obtained by using a new optical setup for compensating drift, and it was confirmed that the half width had a correlation with the bright-field image while the peak shift was no correlation. These results suggest that the viscosity was correctly measured but the elasticity could not be measured because the amount of the peak shift was too small to measure.

Academic Significance and Societal Importance of the Research Achievements

本研究は、光を用いてリモートに直接触ることなく細胞の硬さを定量的に測定できるブリルアン顕微鏡の開発を目的として行われた。実際に細胞内の異なる部位を測定することで硬さの違いを測定でき、さらに画像を取得することで粘性の分布を定量的に測定することに成功した。これらの研究成果から、1細胞レベルでの癌診断の実現可能性が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (28 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (23 results) (of which Int'l Joint Research: 13 results,  Invited: 6 results)

  • [Journal Article] Optical Characterization of Ultra-Thin Films of Azo-Dye-Doped Polymers Using Ellipsometry and Surface Plasmon Resonance Spectroscopy2021

    • Author(s)
      Andam Najat、Refki Siham、Ishitobi Hidekazu、Inouye Yasushi、Sekkat Zouheir
    • Journal Title

      Photonics

      Volume: 8 Issue: 2 Pages: 41-41

    • DOI

      10.3390/photonics8020041

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deuterated Glutamate-Mediated Neuronal Activity on Micro-Electrode Arrays2020

    • Author(s)
      Minoshima Wataru、Masui Kyoko、Tani Tomomi、Nawa Yasunori、Fujita Satoshi、Ishitobi Hidekazu、Hosokawa Chie、Inouye Yasushi
    • Journal Title

      Micromachines

      Volume: 11 Issue: 9 Pages: 830-830

    • DOI

      10.3390/mi11090830

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optical Trapping of Photosoftened Solid Polymers2020

    • Author(s)
      Ishitobi Hidekazu、Akiyama Tomoko、Sekkat Zouheir、Inouye Yasushi
    • Journal Title

      The Journal of Physical Chemistry C

      Volume: 124 Issue: 47 Pages: 26037-26042

    • DOI

      10.1021/acs.jpcc.0c08068

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Surface Enhanced Visible Absorption of Dye Molecules in the Near-Field of Gold Nanoparticles2020

    • Author(s)
      S. Elhani, H. Ishitobi, Y. Inouye, A. Ono, S. Hayashi, and Z. Sekkat
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 3913-3913

    • DOI

      10.1038/s41598-020-60839-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plasmonic coupled modes in metal-insulator-metal structures for sensing applications2018

    • Author(s)
      N. Andam, S. Refki, S. Hayasi, A. Rahmouni, H. Ishitobi, D. V. Nesterenko, Y. Inouye, and Z. Sekkat
    • Journal Title

      Proceedings of SPIE

      Volume: 10722 Pages: 22-22

    • DOI

      10.1117/12.2319936

    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 伝搬型表面プラズモン共鳴により励起されたローダミン6Gの増強ラマン散乱における共鳴ラマン効果2021

    • Author(s)
      本多 巧一、石飛 秀和、井上 康志
    • Organizer
      第68回応用物理学会春季学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Polymer movement induced by two-color light2020

    • Author(s)
      Hidekazu Ishitobi and Yasushi Inouye
    • Organizer
      SPIE Optics + Photonics 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 伝搬型表面プラズモン共鳴によるローダミン6Gの増強ラマン散乱2020

    • Author(s)
      本多 巧一、石飛 秀和、井上 康志
    • Organizer
      第81回応用物理学会秋季学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 表面プラズモン共鳴を用いたイオン濃度分布の顕微イメージング2020

    • Author(s)
      田中 宏知、増井 恭子、Zouheir Sekkat、石飛 秀和、井上 康志
    • Organizer
      第81回応用物理学会秋季学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 多電極アレイ上の神経回路網における重水素化グルタミン酸に活性化された神経活動特性2020

    • Author(s)
      箕嶋 渉、増井 恭子、谷 知己、名和 靖矩、藤田 聡史、石飛 秀和、細川 千絵、井上 康志
    • Organizer
      第81回応用物理学会秋季学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 重水素置換グルタミン酸濃度に依存した神経自発活動解析2020

    • Author(s)
      箕島 渉、増井 恭子、細川 千絵、谷 知己、石飛 秀和、井上 康志
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 伝搬型表面プラズモンによる増強ラマン散乱2020

    • Author(s)
      本多 巧一、石飛 秀和、井上 康志
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Observation for conformation change of DNA via local surface plasmon resonance2019

    • Author(s)
      Y. Inouye, K. Masui, and H. Ishitobi
    • Organizer
      SPIE Optics + Photonics
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell Raman imaging of hippocampal neuronal cells2019

    • Author(s)
      K. Masui, S. Morishita, T. Hamamoto, W. Minoshima, C. Hosokawa, V. R. Daria, H. Ishitobi, and Y. Inouye
    • Organizer
      Biomedical Raman Imaging 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Two-photon excited fluorescence of Platinum Nanoclusters2019

    • Author(s)
      Y. Nakatani, M. Fujieda, H. Ishitobi, and Y. Inouye
    • Organizer
      Global Nanophotonics 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Raman scattering of Rhodamine 6G enhanced by propagating surface-plasmon resonance on a flat silver film2019

    • Author(s)
      K. Honda, K. Masui, H. Ishitobi, and Y. Inouye
    • Organizer
      Global Nanophotonics 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Highly sensitive ion detection based on propagating surface plasmon resonance for bio-sensing and bio-imaging2019

    • Author(s)
      Y. Inouye, H. Ishitobi, and Z. Sekkat
    • Organizer
      Global Nanophotonics 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 海馬神経細胞のラマン分光計測2019

    • Author(s)
      本多 功一、望月 葵、増井 恭子、名和 靖矩、石飛 秀和、細川千絵、Vincent R. Daria、藤田克昌、井上康志
    • Organizer
      第18回産総研・産技連LS-BT合同発表会
    • Related Report
      2019 Research-status Report
  • [Presentation] 輪帯照明によるブリルアン散乱分光法のS/N比向上2019

    • Author(s)
      吉田 智也、澤 淳史、石飛 秀和、井上 康志
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Determination of metal ion concentration using surface plasmon resonance2018

    • Author(s)
      Tanaka, K, Masui, C. Hosokawa, H. Ishitobi, Z. Sekkat, and Y. Inouye
    • Organizer
      Global Nanophotonics 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fluorescent Imaging of Live Hippocampal Neurons with Platinum Nanoclusters2018

    • Author(s)
      M. Fujieda, K. Masui, C. Hosokawa, H. Ishitobi, and Y. Inouye
    • Organizer
      Global Nanophotonics 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Advanced Surface Plasmon Resonance Sensing for Enormous Enhancement of Resolution and Electric Field2018

    • Author(s)
      Y. Inouye, H. Ishitobi, K. Masui, Z. Sekkat
    • Organizer
      Global Nanophotonics 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fluorescent Imaging of Live Hippocampal Neurons with Platinum Nanoclusters2018

    • Author(s)
      M. Fujieda, K. Masui, C. Hosokawa, H. Ishitobi, and Y. Inouye
    • Organizer
      The 79th Japan Society of Applied Physics Autumn Meeting, JSAP-OSA Joint Symposia
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 海馬神経細胞のラマン分光計測2018

    • Author(s)
      望月 葵、増井 恭子、名和 靖矩、石飛 秀和、細川 千絵、ダリア ヴィンセント、藤田 克昌、井上 康志
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] Photochemical imaging of plasmonically enhanced near-fields using light-induced mass transport in azo-polymer films2018

    • Author(s)
      H. Ishitobi
    • Organizer
      SPIE Optics + Photonics
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent Pt nanoclusters and their applicaiton to bio-imaging2018

    • Author(s)
      H. Ishitobi, K. Masui, and Y. Inouye
    • Organizer
      SPIE Optics + Photonics
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Resolution enhancement of plasmonic sensors by metal-insulator-metal structures2018

    • Author(s)
      Z. Sekkat, S. Refki, S. Hayashi, H. Ishitobi, Y. Inouye, and D. Nesterenko
    • Organizer
      SPIE Optics + Photonics
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 海馬神経細胞のラマン分光イメージング2018

    • Author(s)
      望月 葵、増井 恭子、名和 靖矩、石飛 秀和、細川千絵、Vincent R. Daria、藤田克昌、井上康志
    • Organizer
      応用物理学会関西支部 平成30年度第1回講演会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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