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Detection of gap formation between vascular endothelial cells by biosensor

Research Project

Project/Area Number 18K12061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionHiroshima University

Principal Investigator

Yanase Yuhki  広島大学, 医系科学研究科(薬), 准教授 (40452586)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsバイオセンサ / 表面プラズモン共鳴 / インピーダンス / 慢性蕁麻疹 / 血液凝固 / 補体 / マスト細胞 / 好塩基球 / インピーダンスセンサ / 血管透過性亢進 / 血管透過性評価 / SPRイメージングセンサ
Outline of Final Research Achievements

In this study, we developed a sensor which can detect distribution of refractive index (RI) and impedance changes on a sensor chip without any labeling in a real time manner. Using the impedance sensor, we demonstrated that tissue factor (TF)-expressed on vascular endothelial cells (HUVECs) activate extrinsic coagulation cascade and induce gap formation of vascular endothelial cells in the presence of plasma. Moreover, we clarified TF expression levels are increased in patients with chronic spontaneous urticarial (CSU), and TF expressed on monocytes also induce activation of extrinsic coagulation cascade and then cap formation of vascular endothelial cells in the presence of human plasma by means of impedance sensor.

Academic Significance and Societal Importance of the Research Achievements

本研究により、インピーダンスセンサ等のバイオセンサを利用して血管内外起きるイベントをin vitro検出できることが明らかとなった。さらにその手法を利用して、センサ上に複雑な血管内外の環境を再現し、慢性蕁麻疹が起こる仕組みの一端を明らかにすることに成功した。本技術は、難治性慢性蕁麻疹の詳細な発症機序の解明と、新しい治療法の確立・治療薬の開発につながるものと期待する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Journal Article] Coagulation factors induce human skin mast cell and basophil degranulation via activation of complement 5 and the C5a receptor2021

    • Author(s)
      Yanase Yuhki、Matsuo Yoshimi、Takahagi Shunsuke、Kawaguchi Tomoko、Uchida Kazue、Ishii Kaori、Tanaka Akio、Matsubara Daiki、Ozawa Koichiro、Hide Michihiro
    • Journal Title

      Journal of Allergy and Clinical Immunology

      Volume: 147 Issue: 3 Pages: 1101-1104.e7

    • DOI

      10.1016/j.jaci.2020.08.018

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increase of tissue factor expression on the surface of peripheral monocytes of patients with chronic spontaneous urticaria.2020

    • Author(s)
      Saito R, Yanase Y, Kamegashira A, Takahagi S, Tanaka A, Uchida K, Kawaguchi T, Hide M.
    • Journal Title

      Allergy.

      Volume: 75 Issue: 4 Pages: 971-974

    • DOI

      10.1111/all.14110

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Activation of human peripheral basophils in response to high IgE antibody concentrations without antigens.2019

    • Author(s)
      Yanase Y, Matsuo Y, Kawaguchi T, Ishii K, Tanaka A, Iwamoto K, Takahagi S, Hide M
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 20 Pages: 1-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of SPR imaging-impedance sensor for multi-parametric living cell analysis.2019

    • Author(s)
      Yanase Y, Yoshizaki K, Kimura Kaiken, Kawaguchi T, Hide M.
    • Journal Title

      Sensors

      Volume: 19 Pages: 1-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of TF-triggered activation of the coagulation cascade in the pathogenesis of chronic spontaneous urticarial.2018

    • Author(s)
      Yanase Y, Takahagi S, Hide M.
    • Journal Title

      Curr Treat Options Allergy

      Volume: 5 Pages: 383-391

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Activated extrinsic coagulation factors induce degranulation of human skin mast cells and basophils through complement2020

    • Author(s)
      Yuhki Yanase, Yoshimi Matsuo, Tomoko Kawaguchi, Kaori Ishii, Shunsuke Takahagi, Michihiro Hide
    • Organizer
      JSA/WAO Joint Congress 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of LPS and TNFα on the histamine responsiveness of vascular endothelial cells2020

    • Author(s)
      柳瀬雄輝、亀頭晶子、川口智子、内田一恵、秀 道広
    • Organizer
      第93回日本薬理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 血管内皮細胞のヒスタミン応答性に対するLPSとTNFαの影響2019

    • Author(s)
      柳瀬雄輝、亀頭晶子、川口智子、内田一恵、秀 道広
    • Organizer
      第68回日本アレルギー学会学術大会アレルギー学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 慢性蕁麻疹の病態における好塩基球・マスト細胞の役割とin vitro研究から予想されるオマリズマブの作用機序2019

    • Author(s)
      柳瀬雄輝
    • Organizer
      第68回日本アレルギー学会学術大会アレルギー学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] In vitro研究から予想される慢性蕁麻疹の病態と血液凝固反応の関係2019

    • Author(s)
      柳瀬雄輝
    • Organizer
      Urticaria Expert Summit
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 慢性蕁麻疹の病態におけるIgE抗体と血液凝固反応の役割2019

    • Author(s)
      柳瀬雄輝
    • Organizer
      第49回日本免疫アレルギー学会総会学術大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ヒト好塩基球・マスト細胞の活性化制御機構2018

    • Author(s)
      柳瀬雄輝
    • Organizer
      第117回日本皮膚科学会総会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Synergistic expression of tissue factors on vascular endothelial cells induce intercellular gap formation in the presence of plasma via extrinsic coagulation cascade and PAR-12018

    • Author(s)
      Yuhki Yanase, Satoshi Morioke, Kazumasa Iwamoto, Shunsuke Takahagi, Kazue Uchida, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      WCP2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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