Development of bioactive glasses featuring ability of operating cell functions

• Project/Area Number: 18K12075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Obata Akiko 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (40402656)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生体材料 / ガラス / 無機イオン / 細胞 / 免疫 / 骨形成 / 生体用ガラス / 骨代謝

Outline of Final Research Achievements

The aim of this research is to develop novel glass materials by designing glass systems for operating cell functions using inorganic ions and glass structures using our original method based on electrospinning. Results of cell culture tests using macrophage and mesenchymal stem cells for 7 kinds of ion demonstrated that macrophages varied their reactions depending on the type and the amount of the ion provided to the cells, which influenced the bone-forming ability of mesenchymal stem cells. Sol-gel glasses with a fibrous structure were developed and observed to release ions under physiological environment. The glasses are expected to stimulate cell functions and enhance tissue regeneration with the ions released from them.

Academic Significance and Societal Importance of the Research Achievements

骨などの硬組織を再建する材料として、ケイ酸塩やリン酸塩などのガラス材料が有用と期待されている。これまでは、骨形成を担う細胞（骨芽細胞など）に対するこれら材料、および材料から溶出する無機イオンの影響について研究が進められてきた。しかし、細胞の機能発現に大きく影響を及ぼす免疫系細胞に対する影響はほとんど皆無であった。よって本研究課題で得た成果は、免疫系をも考慮した新規材料の設計指針を提供すると考える。また、ガラス材料の多くは柔軟性や成形性に乏しいため、優れた操作性を兼ね揃えた材料が求められていた。本研究課題で作製に成功した綿状の繊維構造体は新しいタイプの生体活性材料として有用と期待される。

Research Products

• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University of Birmingham/University College London/Imperial College London(英国)
• [Int'l Joint Research] University College London/Imperial College London(英国)
• [Journal Article] Preparation of Calcium Phosphate-Biodegradable Polymer Composites with Ion-Releasing Ability for Enhancing Bone Formation (2021)
  • Author(s): Kawai Akari、Matsubara Takashi、Maeda Hirotaka、Obata Akiko、Kasuga Toshihiro
  • Journal Title: MATERIALS TRANSACTIONS Volume: 62 Issue: 1 Pages: 118-123
  • DOI: 10.2320/matertrans.Y-M2020859
  • NAID: 130007960229
  • ISSN: 1345-9678, 1347-5320
  • Year and Date: 2021-01-01
  • Peer Reviewed / Open Access
• [Journal Article] Electrospun cotton-wool-like silica/gelatin hybrids with covalent coupling (2021)
  • Author(s): Maria Nelson, Francesca Tallia, Samuel J. Page, John V. Hanna, Yuki Fujita, Akiko Obata, Toshihiro Kasuga, Julian R. Jone
  • Journal Title: Journal of Sol-Gel Science and Technology Volume: 97 Issue: 1 Pages: 11-26
  • DOI: 10.1007/s10971-020-05420-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Three-Dimensional Cotton-Wool-Like Polyhydroxybutyrate/Siloxane-Doped Vaterite Composite Fibrous Scaffolds: Effect of Imogolite-Coating on Physicochemical and Cell Adhesion Properties (2020)
  • Author(s): Obata Akiko、Mori Kazuma、Inukai Keiichi、Kato Katsuya、Poologasundarampillai Gowsihan、Kasuga Toshihiro
  • Journal Title: Frontiers in Materials Volume: 7
  • DOI: 10.3389/fmats.2020.00033
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Electrospinning 3D bioactive glasses for wound healing (2020)
  • Author(s): Norris Elizabeth、Ramos-Rivera Carolina、Poologasundarampillai Gowsihan、Clark Joshua P、Ju Qun、Obata Akiko、Hanna John V、Kasuga Toshihiro、Mitchell Christopher A、Jell Gavin、Jones Julian R
  • Journal Title: Biomedical Materials Volume: 15 Issue: 1 Pages: 015014-015014
  • DOI: 10.1088/1748-605x/ab591d
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Combinatorial effects of inorganic ions on adhesion and proliferation of osteoblast‐like cells (2019)
  • Author(s): Obata Akiko、Ogasawara Toru、Kasuga Toshihiro
  • Journal Title: Journal of Biomedical Materials Research Part A Volume: 107 Issue: 5 Pages: 1042-1051
  • DOI: 10.1002/jbm.a.36623
  • Peer Reviewed
• [Presentation] マクロファージ/幹細胞共培養下における金属イオンの骨形成に対する影響 (2021)
  • Author(s): 安江光、李誠鎬、小幡亜希子、春日敏宏
  • Organizer: 日本金属学会2021年春期第168回講演大会
• [Presentation] マクロファージ共培養下における幹細胞の骨分化に対する金属イオンの影響 (2020)
  • Author(s): 安江光、李誠鎬、小幡亜希子、春日敏宏
  • Organizer: 日本金属学会第3回第７分野講演会
• [Presentation] Ga2O3-SiO2-CaOゾルゲルガラスの作製とイオン溶出挙動調査 (2020)
  • Author(s): 犬塚匡哉、Zhao Xingchen、Julian R Jones、小幡亜希子、春日敏宏
  • Organizer: 2020年度日本セラミックス協会東海支部学術研究発表会
• [Presentation] 無機イオンの組み合わせが破骨前駆細胞の分化に及ぼす影響 (2020)
  • Author(s): 髙田将宏、小幡亜希子、春日敏宏
  • Organizer: 日本セラミックス協会2020年年会
• [Presentation] Combinatorial effects of inorganic ions on adhesion and proliferation of osteoblast-like cells (2019)
  • Author(s): Akiko Obata, Toru Ogasawara, Toshihiro Kasuga
  • Organizer: The 13th Pacific Rim Conference of Ceramic Societies (PACRIM13)
  • Int'l Joint Research
• [Presentation] Bioactive sol-gel glasses with controlled ion-release for skin wound healing (2019)
  • Author(s): Daisuke Ishihara, Elizabeth Norris, Julian R Jones, Akiko Obata, Toshihiro Kasuga
  • Organizer: The 36th Intenational Japan-Korea Seminar on Ceramics
  • Int'l Joint Research
• [Presentation] Cellular uptake of silicate species in bone cells (2019)
  • Author(s): Joel Turner, Azadeh Rezaei, Julian Jones, Alexandra Porter, Akiko Obata, Gavin Jell
  • Organizer: 30th Annual Conference of the European Society for Biomaterials together with the 26th Annual Conference of the German Society for Biomaterials (DGBM)
  • Int'l Joint Research
• [Presentation] Understanding cellular uptake of silicate species in bone cells (2019)
  • Author(s): Joel Turner, Azadeh Rezaei, Akiko Obata, Alexandra Porter, Julian Jones, Gavin Jell
  • Organizer: TCES-UKSB2019
  • Int'l Joint Research
• [Presentation] リン酸およびケイ酸イオンによる骨芽細胞様細胞の石灰化への影響 (2019)
  • Author(s): 古屋陸、小幡亜希子、春日敏宏
  • Organizer: 粉体粉末冶金協会2019年度秋季大会（第124回講演大会）
• [Presentation] ケイ酸塩ガラス及びリン酸塩ガラスが骨芽細胞様細胞の石灰化に与える影響 (2019)
  • Author(s): 河合星、小幡亜希子、春日敏宏
  • Organizer: 日本セラミックス協会2020年年会
• [Presentation] 無機イオンの組み合わせが破骨前駆細胞に及ぼす影響 (2019)
  • Author(s): 髙田将宏、小幡亜希子、春日敏宏
  • Organizer: 日本セラミックス協会東海支部学術研究発表会
• [Presentation] 免疫細胞への溶出イオンによる影響 (2019)
  • Author(s): 小幡亜希子、尾関祐輝、井口真樹人、春日敏宏
  • Organizer: 日本セラミックス協会2019年年会
• [Presentation] 無機イオンによる免疫細胞機能への影響 (2018)
  • Author(s): 尾関祐輝、井口真樹人、小幡亜希子、春日敏宏
  • Organizer: 第22回生体関連セラミックス討論会
• [Presentation] Osteoblast-like cell responses to silicate ions with different ion structures (2018)
  • Author(s): Akiko Obata, Yamato Goto, Gavin Jell, Toshihiro Kasuga
  • Organizer: Bioceramics30
  • Int'l Joint Research