Nano-sized MRI contrast agents for ultra-sensitive imaging of tumor microenvironment
| Project/Area Number |
18K12079
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 90120:Biomaterials-related
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
Kawano Takahito 九州大学, 先端医療オープンイノベーションセンター, 特任講師 (90526831)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | MRI / 造影剤 / タンパク質 / 癌 / ナノ材料 / 画像診断 / 膵癌 / ナノカプセル / DDS / タンパク質カプセル / 分子イメージング / 画像診断システム |
|---|
| Outline of Final Research Achievements |
MRI (Magnetic Resonance Imaging) has the advantage of spatial resolution and non-invasiveness, but it has the disadvantage of sensitivity in detecting disease sites. In this study, we investigated to develop a new functional MRI nano-contrast agent that can improve the accuracy and sensitivity of MRI diagnosis, visualize the microenvironment of the tumor, and enable functional diagnosis of pathological conditions. Protein nanocapsules were used as the base of the MRI nanocontrast agent, and tumor-selective iRGD peptides were incorporated into the surface of the nanocapsules and gadolinium molecules were encapsulated inside. When the nanocapsules were administered to tumor-bearing mice , it was confirmed that the tumor could be detected using MRI. We also confirmed that the nanocapsules were almost free of cytotoxicity.
|
| Academic Significance and Societal Importance of the Research Achievements |
ナノカプセルは細胞毒性が低く、その体内動態は特に指向性もなく1時間程度で腎排出されることが分かっているが、今回、分子イメージング技術を用いてカプセル表面に腫瘍特異性iRGDペプチドを提示することで、MRIにより病変部位の検出が可能となった。このナノ造影剤を用いることによって、超早期画像診断の実現や、これまで組織レベルまで拡大進行した病変しか検出できなかった画像診断を、細胞から分子レベルの病変で見いだすMRIの機能診断への可能性を秘めていると考えられる。
|
Report
(4 results)
Research Products
(13 results)
-
[Journal Article] Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells2020
Author(s)
Asai, D, Kawano, T, Murata, M, Nakashima, H, Toita, R, Kang, J.H
-
Journal Title
Journal of Oleo Science
Volume: 69
Issue: 3
Pages: 255-260
DOI
NAID
ISSN
1345-8957, 1347-3352
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Histidine-rich Glycoprotein alleviate Ischemia/Reperfusion Liver Injury in Mice with Nonalcoholic Steatohepatitis.2020
Author(s)
Guo J, Akahoshi T, Mizuta Y, Murata M, Narahara S, Kawano T, Nagao Y, Zhang S, Tomikawa M, Kawanaka H, Hashizume M.
-
Journal Title
Liver Transpl.
Volume: Epub
Issue: 6
Pages: 840-853
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Ultrasensitive MRI detection of spontaneous pancreatic tumors with nanocage-based targeted contrast agent.2018
Author(s)
Kawano T, Murata M, Kang JH, Piao JS, Narahara S, Hyodo F, Hamano N, Guo J, Oguri S, Ohuchida K, Hashizume M
-
Journal Title
Biomaterials
Volume: 152
Pages: 37-46
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
