| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
The aim of this study was to develop a simple method to produce gene-loaded lipid/calcium carbonate hybrid nanoparticles (Lions NPs) for application in cancer gene therapy. The particles are designed to efficiently release genes into the cytoplasm by disintegration of calcium carbonate in endosomal acidic pH. For the preparation of Lions NPs, an ethanol injection-based method was devised to form complex nanoparticles in a single step by simply mixing the solutions. The prepared Lions NPs showed high transfection efficiency in the human hepatocellular carcinoma cell line HepG2, comparable to commercially available gene delivery reagents.
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