Quantitative diagnosis of non-alcoholic steatohepatitis by ultrasound and elucidation of the mechanism of occurrence by ultratrace mass spectrometry
| Project/Area Number |
18K12119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90130:Medical systems-related
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
秋山 いわき 同志社大学, 生命医科学部, 教授 (80192912)
池川 雅哉 同志社大学, 生命医科学部, 教授 (60381943)
飯島 尋子 兵庫医科大学, 医学部, 教授 (80289066)
吉田 憲司 千葉大学, フロンティア医工学センター, 准教授 (10572985)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | NASH / 胆汁酸 / メダカ / 腸肝循環 / イメージング質量分析 / 超音波 / 消化器系 / 超微量質量分析 / イメージング / 多変量解析 / 非アルコール性脂肪肝炎 / 超音波診断 / 温度変化 / 遺伝因子 / 非アルコール性脂肪性肝炎（NASH） / 生体組織音速 / 鑑別診断 / るメタボローム解析 |
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| Outline of Final Research Achievements |
A new system to elucidate the pathogenic basis of non-alcoholic hepatitis from the metabolic aspect in detail using a medaka model is developed. Specifically, the main bile acid of medaka, which is rare for fish, is C24 (TCA) and C27 (THCA), and succeeded in visualizing the distribution of these two bile acids within one medaka individual. In addition, the main component analysis revealed the site and metabolism of each digestive organ. Regarding the relationship It was suggested that these two bile acids are functioning. Using this research method, it will be possible to comprehensively analyze the enterohepatic circulation of bile including bile acids, which will lead to the elucidation of the molecular basis from fatty liver to carcinogenesis in the model of one individual medaka.
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| Academic Significance and Societal Importance of the Research Achievements |
肝がん（HCC）への進行性が注目されている非アルコール性脂肪性肝（NASH）の発生機序の解明を胆汁酸の分布に着目し，メダカを対象種として実験的な検討を行った．メダカ成魚の一個体全体に対して12μm厚の全身凍結切片を作製し，消化器系における顕微質量計を用いてイメージング質量分析を行い，肝臓・胆嚢・上部～下部腸官領域で得られたスペクトラムの特徴を多変量解析や機械学習法を応用した画像統計ソフトを用いて特定した．この結果，胆汁酸の腸肝循環の解明がNASHの発生機序解明に繋がる可能性が示唆された．
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Usefulness of Attenuation Imaging with an Ultrasound Scanner for the Evaluation of Hepatic Steatosis.2019
Author(s)
Tada T, Iijima H, Kobayashi N, Yoshida M, Nishimura T, Kumada T, Kondo R, Yano H, Kage M, Nakano C, Aoki T, Aizawa N, Ikeda N, Takashima T, Yuri Y, Ishii N, Hasegawa K, Takata R, Yoh K, Sakai Y, Nishikawa H, Iwata Y, Enomoto H, Hirota S, Fujimoto J, Nishiguchi S.
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Journal Title
Ultrasound Med Biol.
Volume: 45(10)
Issue: 10
Pages: 2679-2687
DOI
Related Report
Peer Reviewed
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[Journal Article] Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.2019
Author(s)
Nakamura N, Hatano E, Iguchi K, Sato M, Kawaguchi H, Ohtsu I, Sakurai T, Aizawa N, Iijima H, Nishiguchi S, Tomono T, Okuda Y, Wada S, Seo S, Taura K, Uemoto S, Ikegawa M.
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Journal Title
BMC Cancer.
Volume: 19(1)
Issue: 1
Pages: 621-621
DOI
Related Report
Peer Reviewed
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[Journal Article] Liver Fibrosis Markers as Assessed by Ultrasound Elastography and Serum Samples: A Large Comparative Study in Hepatitis Virus B and C Liver Diseases2019
Author(s)
Nishimura T, Iijima H, Nishikawa H, Kondo R, Yano H, Kage M, Aoki T, Nakano C, Yuri Y, Ishii N, Hasegawa K, Takata R, Yoh K, Sakai Y, Takashima T, Aizawa N, Ikeda N, Iwata Y, Enomoto H, Hirota S, Fujimoto J, Nishiguchi S.
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Journal Title
Hepatol Res .
Volume: 49(7)
Issue: 7
Pages: 721-730
DOI
Related Report
Peer Reviewed
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[Journal Article] Changes in liver stiffness and steatosis among patients with hepatitis C virus infection who received direct-acting antiviral therapy and achieved sustained virological response2018
Author(s)
Kobayashi N, Iijima H, Tada T, Kumada T, Yoshida M, Aoki T, Nishimura T, Nakano C, Takata R, Yoh K, Ishii A, Takashima T, Sakai Y, Aizawa N, Nishikawa H, Ikeda N, Iwata Y, Enomoto H, Hirota S, Fujimoto J, Nishiguchi S.
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Journal Title
Eur J Gastroenterol Hepatol
Volume: 30
Pages: 546-551
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pathological findings that contribute to tissue stiffness in the spleen of liver cirrhosis patients2018
Author(s)
Kondo R, Kage M, Iijima H, Fujimoto J, Nishimura T, Aizawa N, Akiba J, Naito Y, Kusano H, Nakayama M, Mihara Y, Tanigawa M, Yano H.
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Journal Title
Hepatol Res.
Volume: 48
Pages: 1000-1007
Related Report
Peer Reviewed / Open Access
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[Presentation] Usefulness of Superb Micro Vascular Imaging in the Diagnosis of Focal NodularHyperplasia2018
Author(s)
Takashi Nishimura1, Mariko Hashimoto, Chikage Nakano, Nobuhiro Aizawa, Naoto Ikeda, TomoyukiTakashima, Hiroki Nishikawa, Yoshinori Iwata, Hirayuki Enomoto, Jiro Fujimoto, Shuhei Nishiguchi Hiroko Iijima.
Organizer
The 69th Annual Meeting of the American Association for the Study of Liver Diseases
Related Report
Int'l Joint Research
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