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A theoretical study of self-organization phenomena of 3D membrane deformation: toward understanding of macropinocytosis

Research Project

Project/Area Number 18K13514
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 13040:Biophysics, chemical physics and soft matter physics-related
Research InstitutionCenter for Novel Science Initatives, National Institutes of Natural Sciences (2020)
The University of Tokyo (2018-2019)

Principal Investigator

Saito Nen  大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 特任准教授 (20726236)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords細胞膜変形 / 自己組織化 / 反応拡散現象 / 自己組織化現象 / ソフトマター / フェイズフィールド法 / 反応拡散 / マクロピノサイトーシス / エンドサイトーシス / 3次元膜変形 / 反応拡散系
Outline of Final Research Achievements

Macropinocytosis is an actin-dependent endocytosis, in which the cup-shaped plasma membrane extensions forms without external physical support as in phagocytosis or curvature inducing proteins as in clathrin-mediated endocytosis. To understand this three-dimensional membrane deformation, we developed a novel computational framework that describes the coupling between reaction-diffusion processes of active signaling patches and membrane deformation. Our model exhibits a variety of known morphology dynamics in macropinocytosis. Furthermore, our model can also explains cell movements on the convex curved substrate. Through the analysis of the model, we proposed a novel mechanism of membrane deformation, which occurs through interplay between the membrane deformation and the reaction-diffusion phenomenon on the membrane.

Academic Significance and Societal Importance of the Research Achievements

マクロ飲作用は、細胞が細胞外液を取り込む現象であり、癌細胞の成長、免疫監視、ウィルスの侵入など、あらゆる場面で重要な役割を果たしている。溶液の取り込みを可能にするマイクロメートルスケールの細胞膜の大変形がどのようにして可能であるかは未知であったが、本研究課題による数理的な解析の結果、細胞膜上のシグナル因子の空間パターンと協働して膜変形が起こることが重要であることが明らかになった。この細胞膜変形の新しいメカニズムにより様々な細胞膜変形現象や細胞の遊走性をも説明できる可能性がある。また提案手法は、免疫監査やウィルス侵入などのシミュレーションへ応用可能であり、医療関連分野の研究へ発展する余地もある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2021 2020 2019 2018

All Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results)

  • [Presentation] 変形可能なアクティブ粒子集団の数理モデル2021

    • Author(s)
      斉藤稔
    • Organizer
      日本物理学会第76回年次大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細胞変形ダイナミクスの数理モデリングとデータ駆動解析2020

    • Author(s)
      斉藤稔,井元大輔,澤井哲
    • Organizer
      日本数理生物学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] フェイズフィールドモデルによるアメーバ細胞の一細胞変形動態シミュレーション2020

    • Author(s)
      斉藤 稔, 澤井 哲
    • Organizer
      日本物理学会 第75回年次大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Phase-field modeling for 3D morphodynamics in macropinocytosis2019

    • Author(s)
      Nen Saito
    • Organizer
      2nd Joint Symposium between UBI & MBI
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Morphodynamic feature space of migrating cells2019

    • Author(s)
      井元 大輔, 斉藤 稔, 澤井 哲
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 3D phase field simulation for macropinocytosis of amoeboid cells2019

    • Author(s)
      斉藤 稔, 澤井 哲
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Quantitation of cell shape by machine learning2019

    • Author(s)
      堤 真人, 古澤 力, 澤井 哲, 斉藤 稔
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Phase-field modeling for 3D dynamics in macropinocytosis2019

    • Author(s)
      Nen Saito, Satoshi Sawai
    • Organizer
      The 20th International Conference on Systems Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Phase field model for macropinocytosis of amoeboid cells2018

    • Author(s)
      Nen Saito
    • Organizer
      Joint Symposium between UBI & MBI, Singapore
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Phase-field modeling for 3D morphodynamics of macropinocytosis2018

    • Author(s)
      Nen Saito
    • Organizer
      Macropinocytosis in physiology, disease and therapy, England
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Self organization via membrane de- formation and reaction diffusion: 3D modeling for macropinocytosis of ameboid cell2018

    • Author(s)
      Nen Saito
    • Organizer
      LMU-UT Joint Workshop ,Tokyo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] アメーバ細胞が示すマクロピノサイトーシス動態の3D数理モデリング2018

    • Author(s)
      斉藤稔
    • Organizer
      第15回 生物数学の理論とその応用 -次世代の数理科学への展開-, 京都
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] フェーズフィールド法によるマクロピノサイトーシス動態の 3D シミュレーション2018

    • Author(s)
      斉藤稔
    • Organizer
      第56回 日本生物物理学会年会 シンポジウム” いきた形の新規生成に挑む、理論モルフォダイナミクス”, 岡山
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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