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Study the response of Cyanobacteria to strong light stress and development of cyanobacterial control measures using light inhibition.

Research Project

Project/Area Number 18K13833
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 22040:Hydroengineering-related
Research InstitutionSaitama University

Principal Investigator

Senavirathna Jayasanka  埼玉大学, 理工学研究科, 助教 (70812791)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアオコ発生 / 藍藻制御 / 極端な光 / 酸化ストレス / 水生植物のアレロパシー / 生理と形態パラメータ / 生物の相互作用 / ストレス回復 / Allelopathy / Complex / Extreme light / Interactions / Light stress / Macrophytes / Stress recovery / Varying light / Antioxidants / Macrophyte Interactions / Oxidative stress / Hydrogen peroxide / Proxidase / Diurnal variation / Light intensity / アオコ / 環境ストレス / 活性酸素
Outline of Final Research Achievements

Cyanobacteria growth can be suppressed using extreme light exposure for a shorter duration. Due to the extreme light, their physiology and growth behaviors were altered. Oxidative stress of P. ambiguum and M. aeruginosa increased with the light intensity. The responses were species-specific. Under long-duration extreme light (850 - 900 PAR), M. aeruginosa cells were adapted to stress. When the stress is removed, cells recovered rapidly. Darkness exposure (30 d) completely discolored M. aeruginosa and P. galeata, but were recovered aggressively. It is understood that extreme light alone cannot continously suppress cyanobacterial growth. The Egeria densa allelopathy combined with light conditions can effectively suppress the M. aeruginosa. The outcome of this research is that cyanobacteria can be suppressed by combining high light exposure and allelopathy.

Academic Significance and Societal Importance of the Research Achievements

水システムでの藍藻の生長は大きな問題である。飲用水問題、中毒、嫌な匂いと審美価値の喪失により、藍藻は社会に影響を与える。しかも、Egeria densaは侵襲的な大型植物である。地球温暖化により、日本を含めてE. densaの伝播が増加する。現在の研究結果により、侵入種の生産利用は生物学的に藍藻の生長を抑制できることが分かった。この方法は藍藻の物理と化学制御と比較して、より安全で費用効果がある。極端な光照射による細胞硬化と藍藻の積極的な曝露後の回復は新たな発見である。生理パラメータと形態パラメータとの関係が得られる。これらの関係は藍藻の生育やストレスのシミュレーションや予測に重要である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2018 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Department of Limnology and Water Tec./Faculty of Fisheries and Marine Sc./University of Ruhuna(スリランカ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Egeria densa Allelopathy on Microcystis aeruginosa Under Different Light Intensities and Preliminary Insight into Inter-Parameter Relationships2021

    • Author(s)
      Mudalige Don Hiranya Jayasanka Senavirathna, Guligena Muhetaer, Keerthi Sri Senarathna Atapaththu, Takeshi Fujino
    • Journal Title

      Water Air and Soil Pollution

      Volume: 232 Issue: 4 Pages: 135-135

    • DOI

      10.1007/s11270-021-05088-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Allelopathic influence of low concentration Microcystis aeruginosa on Egeria densa under different light intensities2020

    • Author(s)
      Mudalige Don Hiranya Jayasanka Senavirathna, Guligena Muhetaer, Liu Zhaozhi, Takeshi Fujino
    • Journal Title

      Chemistry and Ecology

      Volume: 36 Issue: 10 Pages: 1-19

    • DOI

      10.1080/02757540.2020.1798939

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Oxidative Stress and Antioxidant Responses of Phormidium ambiguum and Microcystis aeruginosa Under Diurnally Varying Light Conditions2020

    • Author(s)
      Guligena Muhetaer, Mudalige Don Hiranya Jayasanka Senavirathna, Takeshi Fujino
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 6 Pages: 890-890

    • DOI

      10.3390/microorganisms8060890

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of Light Intensity and Exposure Period on the Growth and Stress Responses of Two Cyanobacteria Species: Pseudanabaena galeata and Microcystis aeruginosa2020

    • Author(s)
      Guligena Muhetaer, Takashi Asaeda, Senavirathna M.D.H. Jayasanka, Mahendra B. Baniya, Helayaye D.L. Abeynayaka, M. Harun Rashid, HongYu Yan
    • Journal Title

      Water

      Volume: 12(2), 407 Issue: 2 Pages: 407-407

    • DOI

      10.3390/w12020407

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] EFFECTS OF DIFFERENT LIGHT INTENSITY ON THE GROWTH AND STRESS RESPONSES OF PAEUDANABAENA GALEATA (CYANOBACTERIA)2018

    • Author(s)
      GULIGENA MUHETAER, TAKASHI ASAEDA, HELAYAYE DAMITHA LAKMALI ABEYNAYAKA, SENAVIRATHNA MDH JAYASANKA
    • Organizer
      International Symposium on Ecohydraulics, 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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