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Elucidation of the adhesion mechanism of the non-specific adhesion protein AtaA

Research Project

Project/Area Number 18K14062
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionNagoya University

Principal Investigator

Yoshimoto Shogo  名古屋大学, 工学研究科, 研究員 (90806291)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords接着 / 吸着 / タンパク質 / 微生物 / 微生物接着 / オートトランスポーター / 付着 / QCM
Outline of Final Research Achievements

The purpose of this study is clarification of the adhesion mechanism of AtaA, which exhibits non-specific and high adhesiveness. Through the analysis of the functional sites and the adhesion-driving force of AtaA, the following results were obtained. (1) Adhesion analysis using a quartz crystal microbalance (QCM) revealed the adhesion-driving force of AtaA. (2) By constructing mutants of AtaA and evaluating their adhesive properties, the functional sites were determined. (3) Based on the above findings, we succeeded in reconstructing a protein that exhibits AtaA-like adhesion properties.

Academic Significance and Societal Importance of the Research Achievements

非特異的ながら迅速かつ強固であるというAtaAの水中接着特性は他に類をみない。既知のメカニズムでは説明しくいAtaAの非特異的接着機構に関する知見は、生体分子と材料表面との相互作用を理解し制御する上で重要なものである。また、AtaAを利用することで細菌やペプチド、酵素、リポソームなどのバイオ材料を任意の材料表面に対して簡便に固定化・修飾できるため、その接着を理解することは応用技術の発展をもたらす。水中かつ温和な条件で取り扱う必要のあるバイオ材料のための新規固定化法として、バイオデバイス開発の加速を後押しすることが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Open Access: 1 results,  Peer Reviewed: 4 results) Presentation (12 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Single-Cell Adhesion Force Mapping of a Highly Sticky Bacterium in Liquid2021

    • Author(s)
      Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori
    • Journal Title

      ChemRxiv

      Volume: -

    • DOI

      10.26434/chemrxiv.14221709

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] Native display of a huge homotrimeric protein fiber on the cell surface after precise domain deletion2020

    • Author(s)
      Aoki Sota、Yoshimoto Shogo、Ishikawa Masahito、Linke Dirk、Lupas Andrei、Hori Katsutoshi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 129 Issue: 4 Pages: 412-417

    • DOI

      10.1016/j.jbiosc.2019.09.022

    • NAID

      40022216066

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Process Description of an Unconventional Biofilm Formation by Bacterial Cells Autoagglutinating through Sticky, Long, and Peritrichate Nanofibers2020

    • Author(s)
      Furuichi Yoshihide、Yoshimoto Shogo、Inaba Tomohiro、Nomura Nobuhiko、Hori Katsutoshi
    • Journal Title

      Environmental Science & Technology

      Volume: 54 Issue: 4 Pages: 2520-2529

    • DOI

      10.1021/acs.est.9b06577

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA2019

    • Author(s)
      Noba Kosaku、Ishikawa Masahito、Uyeda Atsuko、Watanabe Takayoshi、Hohsaka Takahiro、Yoshimoto Shogo、Matsuura Tomoaki、Hori Katsutoshi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 141 Issue: 48 Pages: 19058-19066

    • DOI

      10.1021/jacs.9b09340

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Control of AtaA-mediated bacterial immobilization by casein hydrolysates2019

    • Author(s)
      Y. Ohara, S. Yoshimoto, K. Hori
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 128 Issue: 5 Pages: 544-550

    • DOI

      10.1016/j.jbiosc.2019.04.019

    • NAID

      40022069844

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 微生物の非特異的な細胞接着2019

    • Author(s)
      吉本 将悟
    • Journal Title

      生物工学会誌

      Volume: 97 Pages: 87-87

    • Related Report
      2018 Research-status Report
  • [Presentation] Adhesion force mapping of cell surface of high adhesive bacteria using atomic force microscopy2019

    • Author(s)
      Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori
    • Organizer
      The 25th Symposium of Young Asian Biological Engineers’ Community (YABEC 2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inhibition and Detachment of AtaA-Mediated Bacterial Adheasion by Casein Hydrolysates2019

    • Author(s)
      Yuki Ohara, Shogo Yoshimoto, Katsutoshi Hori
    • Organizer
      The 25th Symposium of Young Asian Biological Engineers’ Community (YABEC 2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparison of the adhesiveness of Acinetobacter sp. Tol 5 among various material surfaces2019

    • Author(s)
      Ayane Kawashiri, Shogo Yoshimoto, Taishi Matsushita, Kai Iio, Madoka Takai, Katsutoshi Hori
    • Organizer
      The 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] AFMを用いた高付着性細菌及びその細胞表層タンパク質の接着力測定2019

    • Author(s)
      石井慧、吉本将悟、堀克敏
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] バクテリオナノファイバー蛋白質AtaAで修飾した接着性人工細胞の創出2019

    • Author(s)
      野場考策、石川聖人、植田淳子、渡邉貴嘉、芳坂貴弘、吉本将悟、松浦友亮、堀克敏
    • Organizer
      第13回バイオ関連シンポジウム2019
    • Related Report
      2019 Research-status Report
  • [Presentation] 高付着性蛋白質AtaAで被覆された人工細胞のボトムアップ創成2019

    • Author(s)
      野場考策、石川聖人、植田淳子、渡邊貴嘉、芳坂貴弘、吉本将悟、松浦友亮、堀克敏
    • Organizer
      2019年度 生物工学会中部支部例会
    • Related Report
      2019 Research-status Report
  • [Presentation] 高付着性微生物及び表層タンパク質の原子間力顕微鏡解析2019

    • Author(s)
      石井慧, 吉本将悟, 堀克敏
    • Organizer
      化学工学会 第84年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 高付着性細菌Acinetobacter sp. Tol 5の難付着材料に対する付着性の評価2019

    • Author(s)
      川尻彩音, 吉本将悟, 松下大志, 飯尾魁, 高井まどか, 堀克敏
    • Organizer
      化学工学会 第84年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 高接着性蛋白質AtaA の反復配列を利用した部分欠損体の構築と機能ドメイン探索2018

    • Author(s)
      青木壮太, 吉本将悟, 石川聖人, 堀克敏
    • Organizer
      第70回 日本生物工学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Acinetobacter sp. Tol 5 の難付着材料に対する付着性の評価2018

    • Author(s)
      川尻彩音, 飯尾魁, 吉本将悟, 堀克敏
    • Organizer
      第70回 日本生物工学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] AFMを用いた高付着性蛋白質AtaA及びAtaA被毛微生物の接着特性解析2018

    • Author(s)
      日本化学会 第12回バイオ関連化学シンポジウム
    • Organizer
      石井慧, 吉本将悟, 堀克敏
    • Related Report
      2018 Research-status Report
  • [Presentation] ナノファイバー蛋白質AtaAの接着阻害物質の探索と微生物固定化制御2018

    • Author(s)
      小原優季, 吉本将悟, 堀克敏
    • Organizer
      化学工学会 第50回秋季大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2025-03-27  

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