Elucidation of the adhesion mechanism of the non-specific adhesion protein AtaA
| Project/Area Number |
18K14062
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 接着 / 吸着 / タンパク質 / 微生物 / 微生物接着 / オートトランスポーター / QCM |
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| Outline of Final Research Achievements |
The purpose of this study is clarification of the adhesion mechanism of AtaA, which exhibits non-specific and high adhesiveness. Through the analysis of the functional sites and the adhesion-driving force of AtaA, the following results were obtained. (1) Adhesion analysis using a quartz crystal microbalance (QCM) revealed the adhesion-driving force of AtaA. (2) By constructing mutants of AtaA and evaluating their adhesive properties, the functional sites were determined. (3) Based on the above findings, we succeeded in reconstructing a protein that exhibits AtaA-like adhesion properties.
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| Academic Significance and Societal Importance of the Research Achievements |
非特異的ながら迅速かつ強固であるというAtaAの水中接着特性は他に類をみない。既知のメカニズムでは説明しくいAtaAの非特異的接着機構に関する知見は、生体分子と材料表面との相互作用を理解し制御する上で重要なものである。また、AtaAを利用することで細菌やペプチド、酵素、リポソームなどのバイオ材料を任意の材料表面に対して簡便に固定化・修飾できるため、その接着を理解することは応用技術の発展をもたらす。水中かつ温和な条件で取り扱う必要のあるバイオ材料のための新規固定化法として、バイオデバイス開発の加速を後押しすることが期待される。
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Report
(3 results)
Research Products
(17 results)
