• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Efficient production of plant secondary metabolites through global metabolic engineering in yeast

Research Project

Project/Area Number 18K14069
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionOsaka Prefecture University

Principal Investigator

Yamada Ryosuke  大阪府立大学, 工学(系)研究科(研究院), 准教授 (40608626)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords酵母 / 代謝工学 / 植物二次代謝産物 / CRISPR / セスキテルペン / 変異導入 / ストレス耐性 / 有機溶媒 / 発酵
Outline of Final Research Achievements

The use of genetically modified yeast Saccharomyces cerevisiae to produce a variety of useful chemicals has attracted much attention. Patchoulol is a sesquiterpenoid alcohol and is an industrially important compound. In this study, I aimed to create yeast that can efficiently produce patchoulol by highly expressing the patchoulol synthesis gene in yeast and optimizing the entire mevalonate pathway involved in patchoulol synthesis.
I developed an original method for optimizing a wide range of metabolic pathways in yeast and succeeded in creating yeast that efficiently produces patchoulol by optimizing the expression levels of nine different genes. The constructed yeast produced 42.1 mg/L and 19.8 mg/L/d of patchoulol.

Academic Significance and Societal Importance of the Research Achievements

本研究では、酵母の広範囲の代謝経路を最適化する独自の手法を開発し、パチョロールを効率的に生産する酵母を創製することに成功した。本技術は、解糖経路やメバロン酸経路などを経由する、他の化合物を生産する代謝経路の最適化にも容易に応用可能である。従って、今後は本手法を用い、様々なバイオ燃料、バルクケミカル、ファインケミカルなどの有用物質を効率的に生産する酵母を創製することが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 5 results) Remarks (1 results)

  • [Journal Article] Improvement of lactic acid tolerance by cocktail δ-integration strategy and identification of the transcription factor PDR3 responsible for lactic acid tolerance in yeast Saccharomyces cerevisiae2021

    • Author(s)
      R. Yamada, Y. Kumata, R. Mitsui, T. Matsumoto, H. Ogino
    • Journal Title

      World Journal of Microbiology and Biotechnology

      Volume: 37 Issue: 2 Pages: 19-19

    • DOI

      10.1007/s11274-020-02977-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of 2,3-butanediol tolerance in Saccharomyces cerevisiae by using a novel mutagenesis strategy2021

    • Author(s)
      A. Mizobata, R. Mitsui, R. Yamada, T. Matsumoto, S. Yoshihara, H. Tokumoto, H. Ogino
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 131 Issue: 3 Pages: 283-289

    • DOI

      10.1016/j.jbiosc.2020.11.004

    • NAID

      40022514785

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction of lactic acid-tolerant Saccharomyces cerevisiae by using CRISPR-Cas-mediated genome evolution for efficient d-lactic acid production2020

    • Author(s)
      R. Mitsui, R. Yamada, T. Matsumoto, S. Yoshihara, H. Tokumoto, H. Ogino
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 104 Issue: 21 Pages: 9147-9158

    • DOI

      10.1007/s00253-020-10906-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction of yeast producing patchoulol by global metabolic engineering strategy2020

    • Author(s)
      R. Mitsui, R. Nishikawa, R. Yamada*, T. Matsumoto, H. Ogino
    • Journal Title

      Biotechnology and Bioengineering

      Volume: - Issue: 5 Pages: 1348-1356

    • DOI

      10.1002/bit.27284

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improved stress tolerance of Saccharomyces cerevisiae by CRISPR-Cas-mediated genome evolution2019

    • Author(s)
      R. Mitsui, R. Yamada, H. Ogino
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 189 Issue: 3 Pages: 810-821

    • DOI

      10.1007/s12010-019-03040-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals2019

    • Author(s)
      R. Mitsui, R. Yamada, H. Ogino
    • Journal Title

      World Journal of Microbiology and Biotechnology

      Volume: 35 Issue: 7 Pages: 111-111

    • DOI

      10.1007/s11274-019-2688-8

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Toward the construction of a technology platform for chemicals production from methanol: D-lactic acid production from methanol by an engineered yeast Pichia pastoris2019

    • Author(s)
      R. Yamada, K. Ogura, Y. Kimoto, H. Ogino
    • Journal Title

      World Journal of Microbiology and Biotechnology

      Volume: 35 Issue: 2 Pages: 37-37

    • DOI

      10.1007/s11274-019-2610-4

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Secretory overexpression of the endoglucanase by Saccharomyces cerevisiae via CRISPR-δ-integration and multiple promoter shuffling2019

    • Author(s)
      Y. Sasaki, R. Mitsui, R. Yamada, H. Ogino
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 121 Pages: 17-22

    • DOI

      10.1016/j.enzmictec.2018.10.014

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modulation of gene expression by cocktail δ-integration to improve carotenoid production in Saccharomyces cerevisiae2018

    • Author(s)
      R. Yamada, A. Yamauchi, Y. Ando, Y. Kumata, H. Ogino
    • Journal Title

      Bioresource Technology

      Volume: 268 Pages: 616-621

    • DOI

      10.1016/j.biortech.2018.08.044

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] ゲノム進化法によって創製した熱耐性酵母の遺伝子発現量解析2020

    • Author(s)
      三ツ井良輔,山田亮祐,松本拓也,吉原静恵,徳本勇人,荻野博康
    • Organizer
      極限環境生物学会2020年度 (第21回)年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 点変異・構造変異同時導入による2,3-ブタンジオール耐性酵母の創製2020

    • Author(s)
      溝端明日香,三ツ井良輔,山田亮祐,松本拓也,吉原静恵,徳本勇人,荻野博康
    • Organizer
      極限環境生物学会2020年度 (第21回)年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Construction and analysis of engineered D-lactic acid tolerant Saccharomyces cerevisiae2019

    • Author(s)
      R. Mitsui, R. Yamada, T. Matsumoto, H. Ogino
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Improvement of 2,3-butandiol tolerance in yeast by a novel mutagenesis strategy2019

    • Author(s)
      A. Mizobata, R. Mitsui, R. Yamada, T. Matsumoto, H. Ogino
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Improvement of lactic acid tolerance in the yeast Saccharomyces cerevisiae by optimizing the expression of transcription factors2019

    • Author(s)
      Y. Kumata, R. Mitsui, R. Yamada, T. Matsumoto, H. Ogino
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Modulation of the mevalonate pathway in yeast for efficient patchoulol production by global metabolic engineering2019

    • Author(s)
      R. Yamada, R. Nishikawa, R. Mitsui, T. Matsumoto, H. Ogino
    • Organizer
      18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Simultaneous use of CRISPR-δ-integration and multiple promoter shuffling enhances secretory overexpression of endoglucanase by Saccharomyces cerevisiae2019

    • Author(s)
      Y. Sasaki, R. Mitsui, R. Yamada, T. Matsumoto, H. Ogino
    • Organizer
      The 10th International Symposium of Innovative BioProduction Kobe
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] δ-CRISPR法とプロモーターシャッフリング法を融合した酵母によるタンパク質高生産技術2018

    • Author(s)
      佐々木優果,三ツ井良輔,山田亮祐,荻野博康
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Research-status Report
  • [Remarks] 大阪府立大学 化学工学課程/化学工学分野 反応工学グループ

    • URL

      http://www2.chemeng.osakafu-u.ac.jp/group4/indexj/

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi