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Development of quantitative analysis for structure change of single-molecule junction

Research Project

Project/Area Number 18K14091
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 28030:Nanomaterials-related
Research InstitutionOsaka University

Principal Investigator

Komoto Yuki  大阪大学, 産業科学研究所, 助教 (90814210)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords単分子計測 / MCBJ法 / 機械学習 / 単分子科学 / 単分子接合
Outline of Final Research Achievements

Single-molecule measurements, which measure the tunneling current of a single molecule passing through a metal nanogap, have been researched for the development of measurement methods for biomolecules and the fabrication of molecular devices. However, conventional single-molecule measurement method only analyzed the average conductance and not the change in conductance. In this study, we focused on the time variation of the current in single-molecule measurements and established a technique to identify single molecules using mechanically controllable break junction measurements. By using machine learning to analyze not only the average conductance but also the changes in the pulse current signals obtained by single-molecule measurement, we succeeded in identifying neurotransmitters, DNA modified bases, and DNA base lengths.

Academic Significance and Societal Importance of the Research Achievements

これまで、神経伝達物質やDNA修飾塩基は、生物学的な側面から重要性が認識されていたものの簡便に計測する手法がなく、詳細な理解や医療応用などの幅広い応用ができなかった。本研究により、単分子レベルでこれらの分子を検出することが可能になり、様々な研究に発展していくことが期待される。
また、単分子計測研究の観点からは、これまで解析できていなかった、電流変化という動的な情報を解析することにより、単分子が識別可能である、すなわち、分子固有の構造変化の情報があると示した。これにより、単分子計測を複数種の分子が含まれる溶液中での計測を可能にすることに成功した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Length Discrimination of Homo-oligomeric Nucleic Acids with Single-molecule Measurement2021

    • Author(s)
      KOMOTO Yuki、OHSHIRO Takahito、TANIGUCHI Masateru
    • Journal Title

      Analytical Sciences

      Volume: 37 Issue: 3 Pages: 513-518

    • DOI

      10.2116/analsci.20SCP13

    • NAID

      130007996240

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2021-03-10
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Single-Molecule Electrical Identification Method for Cyclic Adenosine Monophosphate Signaling Pathway2021

    • Author(s)
      Komoto Yuki、Ohshiro Takahito、Taniguchi Masateru
    • Journal Title

      Nanomaterials

      Volume: 11 Issue: 3 Pages: 784-784

    • DOI

      10.3390/nano11030784

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time-resolved neurotransmitter detection in mouse brain tissue using an artificial intelligence-nanogap2020

    • Author(s)
      Komoto Yuki、Ohshiro Takahito、Yoshida Takeshi、Tarusawa Etsuko、Yagi Takeshi、Washio Takashi、Taniguchi Masateru
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 11244-11244

    • DOI

      10.1038/s41598-020-68236-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Key aurophilic motif for robust quantum-tunneling-based characterization of a nucleoside analogue marker2020

    • Author(s)
      Furuhata Takafumi、Komoto Yuki、Ohshiro Takahito、Taniguchi Masateru、Ueki Ryosuke、Sando Shinsuke
    • Journal Title

      Chemical Science

      Volume: 11 Issue: 37 Pages: 10135-10142

    • DOI

      10.1039/d0sc03946b

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of an alcohol-associated cancer marker by single-molecule quantum sequencing2020

    • Author(s)
      Komoto Yuki、Ohshiro Takahito、Taniguchi Masateru
    • Journal Title

      Chemical Communications

      Volume: 56 Issue: 91 Pages: 14299-14302

    • DOI

      10.1039/d0cc05914e

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-Molecule Counting of Nucleotide by Electrophoresis with Nanochannel-Integrated Nano-Gap Devices2020

    • Author(s)
      Ohshiro Takahito、Komoto Yuki、Taniguchi Masateru
    • Journal Title

      Micromachines

      Volume: 11 Issue: 11 Pages: 982-982

    • DOI

      10.3390/mi11110982

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dissecting Time-Evolved Conductance Behavior of Single Molecule Junctions by Nonparametric Machine Learning2020

    • Author(s)
      Liu Bo、Murayama Sanae、Komoto Yuki、Tsutsui Makusu、Taniguchi Masateru
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 11 Issue: 16 Pages: 6567-6572

    • DOI

      10.1021/acs.jpclett.0c01948

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 機械学習を用いた解析による単分子神経伝達物質検出2020

    • Author(s)
      小本祐貴
    • Organizer
      第4回計測インフォマティクス研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 単分子計測によるマウス脳内神経伝達物質検出2020

    • Author(s)
      小本祐貴,大城 敬人,吉田 剛, 足澤 悦子,八木 健,鷲尾 隆,谷口 正輝
    • Organizer
      応用物理学会第67回春季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Single biomolecule detection and discrimination with nanogap measurements2020

    • Author(s)
      Yuki Komoto
    • Organizer
      The 23rd SANKEN International Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 単分子計測によるアルデヒド化修飾塩基の検出2019

    • Author(s)
      小本祐貴、大城 敬人、谷口 正輝
    • Organizer
      応用物理学会第80回秋季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Single-molecule Discrimination of monoamine neurotransmitters with MCBJ method2019

    • Author(s)
      Yuki Komoto, Takahito Ohshiro, Takayuki Takaai, Takeshi Yoshida, Takashi Washio, Masateru Taniguchi
    • Organizer
      10th International Conference on Molecular Electronics & BioElectronics
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] MCBJ法を用いた神経伝達物質の単分子識別2019

    • Author(s)
      小本 祐貴
    • Organizer
      応用物理学会 有機分子・バイオエレクトロニクス分科会研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Single-molecule discrimination of neurotransmitters with machine learning2019

    • Author(s)
      Yuki Komoto,  Takahito Ohshiro, Takayuki Takaai, Takeshi Yoshida, Takashi Washio, Masateru Taniguchi
    • Organizer
      1st AIRC Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-molecule recognition of monoamine neurotransmitters with machine learning2019

    • Author(s)
      Yuki Komoto, Takahito Ohshiro, Takayuki Takaai, Takeshi Yoshida, Takashi Washio, Masateru Taniguchi
    • Organizer
      日本化学会第99回春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] MCBJ法を用いたDNAシーケンシングによる連続塩基数識別法の開発2019

    • Author(s)
      小本祐貴,大城敬人,谷口正輝
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] 機械学習を用いた神経伝達物質の一分子識別2018

    • Author(s)
      小本祐貴,大城敬人,鷹合孝之,吉田剛,鷲尾隆,谷口正輝
    • Organizer
      第12回分子科学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] MCBJ法を用いた神経伝達物質の機械学習識別2018

    • Author(s)
      小本祐貴,大城敬人,鷹合孝之,吉田剛,鷲尾隆,谷口正輝
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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